Repositorio Digital

Resumen en ingles

Background: Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary.Objective: To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllusessential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells.Methods: The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthe-sis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhi-bition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cyto-toxic activity.Results: MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC5031.00 ± 0.61 μg/mL.Conclusions: Our study indicates an interesting bioactivity of isoeugenol and meth-ylisoeugenol against M. canis, M. gypseum and mammalian cells.