Examinando por Autor "Caicedo Cepeda, Juan Carlos"
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- PublicaciónAcceso abiertoBactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity(Frontiers in Microbiology, 2020, 2020-06-24) Villamizar, Sonia; Ferro, Jesus Aparecido; Caicedo Cepeda, Juan Carlos; Alves, L. M. C.; CIBASThe bacterium Pseudomonas entomophila has been recognized as an exceptional species within the Pseudomonas genus, capable of naturally infecting and killing insects from at least three different orders. P. entomophila ingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated a P. entomophila bacterial strain from soil samples which displayed a strong activity against Xanthomonas citri subsp, citri (Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria against Xcc and its ability to reduce citrus canker severity was assessed both ex planta and in planta. Our findings show that pathogenicity assays in Citrus x limonia by pressure infiltration and spray with a mixture of P. entomophila and Xcc leaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity of P. entomophila against a phytopathogenic bacterium. Collective action of P. entomophila factors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that the P. entomophila strain could be a promising biocontrol agent acting directly against Xcc.
- PublicaciónAcceso abiertoBiological Control of Citrus Canker(London : IntechOpen, 2019., 2019-08-23) Caicedo Cepeda, Juan Carlos; Villamizar Cancelado, Sonia; Snjezana Topolovec-PintaricCitrus canker is a disease that affects the major types of commercial citrus crops. Xanthomonas citri subsp. citri, the etiological agent, reaches to mesophyll tissue through the stomata and afterward induces cell hyperplasia. Disease management has been based on both tree eradication and copper spray treatment. Overuse of copper for control of bacterial citrus canker has led to the development and prevalence of copper-resistant strains of Xcc. Several genera of both soil- and plant-associated bacteria became powerful tools in sustainable agriculture for control of Xcc and reduction of citrus canker disease severity. In this chapter we present bacteria able to interfere with quorum sensing as well to display antibacterial activity against Xcc by production of secondary metabolite. These bacteria may represent a highly valuable tool in the process of biological control and offer an alternative to the traditional copper treatment currently used for the treatment of citrus canker disease, with significant environmental, economic, and health implications worldwide.