Examinando por Autor "Ríos Ramírez, Yesmit-Karina."
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- PublicaciónRestringidoAcanthamoeba SPP como hospedero de helicobacter pylori en diferentes tipos de agua de SAN JOSE DE CÚCUTA, NORTE DE SANTANDER, 2018-2019(Cúcuta: Universidad de Santander, 2019, 2019-10-22) Bautista, Maria Fernanda.; Espinel Leal, Maria Fernanda.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.The amoebae of free life are cosmopolitan protozoa that are found in humid environments such as soil and water, being the most common that they are found in water sources, whether they are sweet or salty but most prevalent in stagnant waters. Among the free-living amoebas that have a pathogenic capacity for man, those of the genus Acanthamoeba spp and Naegleria spp stand out, and Acanthamoeba spp also has an endosymbiotic role of great relevance with other mainly pathogenic microorganisms. As is Helicobacter pylori which is a gram-negative, spiral-shaped and mobile gram bacillus that is colonizing the stomach of approximately half of the world's population, it has been linked as the causative agent of chronic diseases such as gastritis but in greater relevance with gastric cancer.
- PublicaciónRestringidoAsociación de factores de riesgo con la presencia de Anticuerpos tipo IGG ANTI- Toxoplasma Gondii en estudiantes con problemas Oculares de la UNIVERSIDAD DE SANTANDER CÚCUTA 2018-19.(Cúcuta: Universidad de Santander, 2019, 2019-07-28) Forgione Rincón, Laura Andrea.; Tarazona Duran, Greicy Anyobriany.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Association of risk factors with the presence of IgG antibodies ANTI-Toxoplasma gondii in students with eye problems at the University of Santander-Cúcuta. Toxoplasmosis, caused by the protozoan parasite Toxoplasma gondii, is one of the most common parasitic infections in humans and other warm-blooded animals. It has been found throughout the world and almost a third of humanity has been exposed to this parasite and in most adults, it does not cause serious diseases, but it can cause some adverse effects in children with congenital infection and devastating diseases in immunocompromised individuals ; hence the importance of conducting this study in which we sought to determine the risk factors that are associated with the presence of anti-Toxoplasma gondii IgG antibodies in individuals with eye problems at the University of Santander, Cúcuta; this research intends, in addition to contributing objective results, to make an initial diagnosis that can serve as a starting point for other deeper research works, which manage to consider a greater quantity of both qualitative and quantitative variables. An instrument previously validated by an expert was applied to 120 students from the University of Santander, Cúcuta. After that, samples of peripheral blood were taken to each of them, which was centrifuged for the extraction of the serum, which was stored at -20 ° C for further processing. The ELISA was carried out in order to determine the presence of anti-Toxoplasma gondii IgG antibodies in the samples taken from the target population. In the tests carried out, a percentage of prevalence of infection by T. gondii of 18.3% was determined. On the other hand, with respect to the results obtained through the survey, the existence of a significant association between seropositivity and three risk factors studied was demonstrated, such as the socioeconomic stratum, the habit of fruit consumption and the frequency of food consumption for sale on public roads.
- PublicaciónRestringidoDeterminación de Amebas de vida libre y Bacterias Endosimbióticas en agua de consumo de los Municipios de Cúcuta y los Patios.(Cucúta: Universidad de Santander, 2018, 2018-12-10) Rodriguez Daza, Karen Yesenia.; Albarracin Salcedo, Leidy Johanna.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebae are characterized by microorganisms of cosmopolitan distribution, are involved in infections that affect the central nervous system, disseminated skin infections and keratitis. These protozoa have the ability to form endosymbiotic relationships with whole pathogenic microorganisms and also possess the capacity to form biofilms, which make them difficult complexes to eliminate from established environments, the importance of this study is based on analyzing and identifying AVL in drinking water of the municipalities of Cúcuta and the patios.
- PublicaciónRestringidoDeterminación de amebas de vida libre y de bacterias endosimbióticas potencialmente patógenas en aguas del área RURAL DEL RIO PAMPLONITA, NORTE DE SANTANDER.(Cúcuta: Universidad de Sántander, 2018, 2018-05-23) Avellaneda Figueroa, Yendy Andriyirley; Ramírez Tocora, Jorge Andrés.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebae (FLA) are ubiquitous microorganisms’ that can affect to human being cause several pathologies, through their resistance mechanisms, which is really hard “thermotolerance”. However, this microorganisms phagocyte another ones: fungi, bacteria and virus, but the last ones can do endosymbiotic relationship, and the amoebae confers resistance to their habitat and increase the pathogenic potential, that’s why it constitutes a huge problem to Public Health. The aim of this study was demonstrate the presence of free living amoebae and endosymbiotic bacterias potentially pathogenic in samples: Bocatoma El Rosal, Monteadentro, La Naciente, Estación Limnimétrica Curva Los Adioses, Pamplonita, El Diamante and Pamplonita, to characterize their distribution. Developing a field-descriptive study through of recollection of water samples and filtrated to inoculate No nutritive Agar enrichment with inactive Escherichia coli.
- PublicaciónRestringidoDeterminación de vermamoeba vermiformis y bacterias gram negativas patógenas endosimbióticas en muestras de suelo en la CIUDAD DE CÚCUTA.(Cúcuta: Universidad de Sántander, 2018, 2018-12-11) Mogollón Muñoz, Manuel Andrés.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebas are ubiquitous microorganisms, which have been linked to deadly pathologies for humans, and their virulent potential is directly linked to their thermotolerant capacity and the relation of endosymbiosis that can be created with bacteria resistant to amoebic lysis, which It can be fatal to health. The objective was to determine the presence of Vermamoeba vermiformis and endosymbiotic pathogenic Gram negative bacteria in soil samples in the city of Cúcuta. To do this, 3 samples were taken in which soil samples were collected from 5 selected sites (UDES, Comfaoriente College, Juan Atalaya School, Fuente Luminosa and Villasilvania) at strategic points of each site; The samples that were collected were seeded on ANN agar with Page solution and inactivated E. coli. When the samples were analyzed, the amoeba Vermamoeba vermiformis was isolated, after which 4 replicas of the positive samples were made with the amoeba to have a pure culture and to be able to identify Gram-negative bacteria. For the identification of Gramnegative bacteria, lysis was performed on the amoeba using a 27-gauge needle so that bacteria could escape from the interior
- PublicaciónRestringidoDETERMINACIÓN in vitro DE LA SENSIBILIDAD A ANTIPARASITARIOS DE Blastocystis spp AISLADOS DE ANIMALES DOMÉSTICOS EN LA CIUDAD DE CÚCUTA 2018(Cúcuta: Universidad de Sántander, 2019, 2019-07-04) Barbosa Mendoza, Jonathan Jaddir; Naranjo Pabón, Jeynny Herminda.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Determinar in vitro la sensibilidad a antiparasitarios de Blastocystis spp aislados de animales domésticos en Cúcuta en 2018. MATERIAL Y METODOS: Se realizó un estudio en el año 2018 en 250 muestras fecales de animales domesticos. La identificación de Blastocystis spp. se realizó en muestras de heces mediante examen microscópico directo y cultivo en medio de Jones modificado. La evaluación de la susceptibilidad in vitro frente a los fármacos Metronidazol, Trimetoprim/ Sulfametoxazol, Secnidazol y Nitazoxanida, se realizó en 20 muestras fecales monoparasitadas con Blastocystis spp. a los cuales se los cultivó en viales, en 4 concentraciones de cada antiparasitario por duplicado, además de un control que contenia el parásito sin agregar el antiparasitario. RESULTADOS: En los 250 coprologicos realizados las muestras que fueron las utilizadas en las pruebas de susceptibilidad solo se lograron obtener 9,6% de muestras monoparasitadas por Blastocystis spp. Se obtuvo mayor susceptibilidad del parásito frente al Secnidazol a una concentracion de 200 µg/mL logro inhibir un 98,28%, y un 81,93% de inhibicion en la concrentracion de 100 µg/mL, en el que se obtuvo otro porcentaje mayor de susceptibilidad fue el Trimetoprim/sulfametoxazol con un 93,80% a una concentracion de 200 µg/mL, y un 67,68% a una concentracion de 100 µg/mL, la Nitazoxanida tuvo un 88,65% a una concentracion de 200 µg/mL, y por ultimo el Metronidazol tuvo un 88,26% a una concentracion de 200 µg/mL y el mas efectivo a concentraciones bajas sobre el parásito fue el Secnidazol con una media de la CL50 55,56 µg/mL CONCLUSIONES: Blastocystis se transmite entre animales y humanos por la ingestión de quistes, presentes en aguas o alimentos contaminados con materia fecal procedente de un portador, la utilizacion de Secnidazol es una muy buena alternativa para la inhibición del Blastocystis spp.
- PublicaciónRestringidoEndosimbiosis de Naegleria SPP y Cianobacterias productoras de Microcistinas aisladas de Ambientes hostiles en NORTE DE SANTANDER.(Cúcuta: Universidad de Santander, 2018, 2018-12-11) Villamizar Avellaneda, Génesis Nathaly.; Escalante Riveros, Jessica Johanna.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Naegleria spp. Amoeba of free life characterized presents pathogenic human species, thermophilic properties and capacity to act as endosimbion of other groups of microorganisms, involves microbial relationships and makes possible the survival of the same to hostile conditions. The microorganisms that have been described as resistant to amoebic lysis, are the cyanobacteria, which are prokaryotes that, among other properties, have been characterized by carrying out photosynthesis, but there is a small group of these microorganisms that carry out a alternating metabolism, triggering the release of microcystins such as type D, described as a hepatotoxin with protumoral activity product of organisms such as Microcystis sp., Planktothrix sp. and Anabaena sp. The objective of this research is to establish the endosymbiosis of Naegleria spp. and cyanobacteria producing microcystins isolated from hostile environments in Norte de Santander. The research was based on 4 phases, in 1 place the collection of the samples in the hostile environments (Residual, Thermal and Rivers).
- PublicaciónRestringidoEndosimbiosis entre acanthamoeba spp y cryptococcus spp. En muestras ambientales de la ciudad de CUCUTA, NORTE DE SANTANDER.(Cúcuta: Universidad de Santander, 2018, 2018-12-12) Tarazona Gutierrez, Danna Franchesca.; Vega Galvis, Diana Gabriela.; Contreras Rangel, Jael.; Ríos Ramírez, Yesmit-Karina.Acanthamoeba spp. and Cryptococcus spp. they are opportunistic pathogens, ubiquitous in nature. Many species of Acanthamoeba spp. they are responsible for fatal diseases for humans such as: meningoencephalitis and keratitis, so the study of their endosymbiotic interaction is representative. The purpose of the study was to establish the endosymbiosis between Acanthamoeba spp. and Cryptococcus spp. in environmental samples from the city of Cúcuta, Norte de Santander. A descriptive study was carried out, collecting a total of 132 samples, 120 of soils from four parks in the city of Cúcuta, Norte de Santander: Colón Park, Santander Park, San Eduardo Park, Erasmo Meoz University Hospital Park and 12 water samples taken from the Pamplonita River and the Bogotá Wastewater Canal. The culture was carried out on non-nutritive agar plates previously seeded with inactive Escherichia coli ATCC 25922. Acanthamoeba spp. it was isolated from 83 (70%) of the soil samples and 12 (100%) of the water samples. Also, the identification of Cryptococcus spp. as an endosymbiont, it was present in 7 (5.8%) of the strains samples of Acanthamoeba spp.
- PublicaciónRestringidoEvaluación de la susceptibilidad a Antiparasitarios de Blastocystis SPP. Aislados en muestras Fecales Humanas en población Monoparasitada de Cúcuta, Norte de Santander 2018.(Cúcuta: Universidad de Santander, 2018, 2018-12-13) Bayona Cárdenas, Yelitza Fernanda .; Alfonso Romero, Alex Santiago.; Contreras Rangel, Jael.; Ríos Ramírez, Yesmit-Karina.OBJETIVO: Describir la prevalencia y susceptibilidad antiparasitaria in vitro de Blastocystis spp. en heces fecales de Cúcuta, Norte de Santander. MATERIAL Y METODOS: Se realizó un estudio en el año 2018 en 106 muestras fecales humanas. La identificación de Blastocystis spp. se realizó en muestras de heces mediante examen microscópico directo y cultivo en medio de jones modificado. La evaluación de la susceptibilidad in vitro frente a los fármacos Metronidazol, Trimetropim/ Sulfametoxazol y Secnidazol, se realizó en 20 muestras fecales monoparasitadas con Blastocystis spp. a los cuales se los cultivó en viales, en 6 concentraciones dobles de cada antiparasitario (desde 250 mg/mL hasta 7,81 mg/mL) para Metronidazol y Secnidazol, para Trimetropim/ Sulfametoxazol (desde 80/400 mg/mL hasta 2.5/12.5 mg/mL) además de un control. RESULTADOS: El 60,37% (64/106) de las muestras tuvo Blastocystis spp, además se observó que, el color pardo y la consistencia pastoza estuvo asociado con la mayor frecuencia del parásito (31.13% y 35,84% respectivamente). La concentración que logro inhibir en un 60% del total de las 20 muestras estudiadas para Metronidazol fue de 12 250 mg/m, Secnidazol inhibió un 90% a 250 mg/mL y un 35% a 125 mg/mL, mientras que el Trimetropim-Sulfametoxazol inhibió solo un 30% a su máxima concentración en este estudio de 80/400 mg/mL. CONCLUSIONES: Blastocystis spp presenta una alta prevalencia en las muestras fecales de Cúcuta, Norte de Santander, mostrando ser importante y un problema de salud pública en la ciudad. Asimismo, las muestras aisladas mostraron ser susceptibles in vitro a altas concentraciones de Metronidazol y Secnidazol por lo que podrían ser de elección para el tratamiento de este parásito.
- PublicaciónRestringidoIdentificación de Acanthamoeba SPP y Naegleria SPP con potencial Patógeno en aguas de siete puntos de muestreo del RIO PAMPLONITA.(Cúcuta: Universidad de Santander, 2017, 2017-06-10) Duque Nossa, Victor Andres.; Gelvis Corzo, Vladimir Alejandro.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Las amebas de vida libre (AVL) se encuentran implicadas en severas patologías humanas, ya sea de manera directa o indirecta, al servir como vehículo para agentes enteropatógenos; de allí la importancia de realizar un estudio donde se buscó determinar la presencia de los dos géneros más relevantes de AVL en aguas de siete puntos del rio pamplonita; estos puntos fueron elegidos de manera estratégica, debido al importante contacto con la actividad humana que ocurre en estos. Se buscó determinar las condiciones fisicoquímicas del agua del Rio Pamplonita, buscar la presencia de Acanthamoeba spp y Naegleria spp en sus aguas mediante observaciones directas y cultivos Se realizaron tres muestreos en cada uno de los siete puntos de recolección de muestra, en un intervalo de tiempo de cinco meses; se realizaron simultáneamente mediciones in situ del pH y temperatura. A las muestras recolectadas se les realizo una observación microscópica directa, en búsqueda de morfologías compatibles con los géneros Acanthamoeba spp y Naegleria spp consiguiente a esto se llevó a cabo un proceso de filtración por membrana sobre dichas muestras, con fin de obtener un sedimento para la realización de cultivos en agar no nutritivo suplementado con E. Coli 25922; adicional a esto se realizó la determinación de coliformes por el método de Petri film. En los cultivos donde se lograron obtener aislados positivos para Acanthamoeba spp y/o Naegleria spp, se realizaron pruebas de osmo y termo tolerancia mediante la exposición de los cultivos a diferentes temperaturas en el caso de la termo tolerancia y así mismo a diferentes concentraciones sorbitol agregadas en el medio para evaluar la osmotolerancia de estas amebas. Terminado el presente estudio se logró determinar la presencia de amebas de vida libre tanto de los géneros Acanthamoeba spp y Naegleria spp, así como también de otros géneros de amebas anfizoicas no tan relevantes en el ámbito clínico. De igual manera, se estableció una relación entre la presencia de estas amebas de vida libre con la presencia de los microorganismos conocidos como coliformes.
- PublicaciónRestringidoIdentificación de tipos de Bacterias Endosimbióticas en Amebas de vida libre aisladas en muestras de suelo del Municipio de TIBU, 2018.(Cucuta: Universidad de Santander, 2018, 2018-12-12) Sanchez Chona, Jonnathan.; Contreras Rangel, Jael.; Ríos Ramírez, Yesmit-Karina.Las amebas de vida libre son microorganismos altamente patógenos que están distribuidos a nivel mundial, estos microorganismos son llamadas anfizoicas, ya que son capaces de vivir en el medio ambiente y actuar como parásitos en el ser humano, pertenecen al grupo de protozoos Rhizopodos del orden amoebida (Acanthamoeba) y del orden Schizopyrenida (Naegleria y Balamuthia), están distribuidos a nivel mundial en diversos reservorios como por ejemplo aguas de piscinas, pozos, estanques, ríos, el agua del aire, entre otros. Este grupo de protozoos produce diferentes patologías como meningoencefalitis amebiana primaria (MAP) por el género Naegleria; encefalitis granulomatosa amebiana (EGA) por los géneros Acanthamoeba, Balamuthia y Sappinia; que son de relevancia clínica ya que pueden comprometer el sistema nervioso central; además, a Acanthamoeba y Balamuthia se les ha relacionado con lesiones oculares como queratitis y úlceras cornéales, así como lesiones primarias de la piel. Como objetivo se planteó Identificar la interacción que presentan las amebas de vía libre con bacterias endosimbióticas presentes en muestras de suelo del municipio Tibú Norte de Santander. para ello se realizó una serie de muestreo ( 3) en cinco sitios escogidos dentro del casca urbano del municipio, las muestras recolectadas se transportaron en medios estériles y fueron sembrados en agares ANN con E.coli inactivada.
- PublicaciónRestringidoIdentificación de tipos de Bacterias Endosimbióticas en Amebas de vida libre aisladas en muestras de suelo del Municipio de TIBÚ, NORTE DE SANTANDER 2018.(Cúcuta: Universidad de Santander, 2019, 2019-07-03) Jauregui Sierra, Karen Tatiana.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebas are highly pathogenic microorganisms distributed worldwide, these microorganisms are called amphizoic, since they are capable of living in the environment and act as parasites in humans, belong to the group of Rhizopod protozoa of the order amoebida (Acanthamoeba ) and of the order Schizopyrenida (Naegleria and Balamuthia), are distributed worldwide in various reservoirs such as swimming pool waters, wells, ponds, rivers, water in the air, among others. This group of protozoa produces different pathologies such as primary amebic meningoencephalitis (MAP) by the genus Naegleria; amebic granulomatous encephalitis (GAS) by the genera Acanthamoeba, Balamuthia and Sappinia; which are of clinical relevance since they can compromise the central nervous system; In addition, Acanthamoeba and Balamuthia have been linked to eye injuries such as keratitis and corneal ulcers, as well as primary lesions of the skin. The objective of this study was to identify the interaction of freeflowing amoebas with endosymbiotic bacteria present in soil samples from Tibú Norte de Santander municipality. for this, a series of sampling (3) was carried out in five selected sites within the urban helmet of the municipality, the collected samples were transported in sterile media and were planted in ANN hangars with inactivated E. coli. The samples had a follow-up of 20 days in which a series of peals were made to obtain a pure culture. A quantity of three rings was needed to obtain the pure culture, which was followed by sowing in biochemical media such as the biochemical batteries of citrate, Lía, Tsi and SIM to observe the amoebic endosymbiosis and verify whether the amoeba harbored bacteria, whether Gram negative or Gram positive. Achieving thus isolating amoebas of the genus Acanthamoeba, Naegleria, Balamuthia, Vermamoeba in our work we observed morphology of Gram negative bacteria product of the endosymbiotic mechanism of the genus Acinetobacter and Pseudomonas. As a conclusion, it was possible to observe the presence of amoebas and highly pathogenic bacteria contained in soil samples, which constitutes an important signal in the people of this municipality and public health entities. Recommending taking the necessary measures of prevention to these highly pathogenic microorganisms.
- PublicaciónRestringidoNiveles sericos de Bioelementos en individuos Blastocystis SPP. positivos en la Ciudad de Cúcuta, Norte de Santander.(Cúcuta: Universidad de Santander, 2017, 2017-11-30) Mayorga Angel, Estefany.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.The parasitic diseases are of great importance to be very frequent, from the point of view of damage; they range from symptomatology to fatal cases. In general, the prevalence of Blastocystis spp. is mayor in developing countries due to poor sanitary infrastructure and / or low level of attention to environmental hygiene, since this is the parasite that can be infected by the ingestion of the cyst form by fecal-oral route. This is why these are some of the reasons why electronic medications are currently acceptable. Several studies have shown that elements such as iron, phosphorus, calcium and magnesium are necessary to maintain vital functions of the organism. These micronutrients are involved in many enzyme systems that function in various biological processes. The main objective of the research study was to establish the serum levels of bioelements in positive Blastocystis spp. Individuals in the city of Cúcuta, Norte de Santander.
- PublicaciónRestringidoPresencia de amebas de vida libre y bacterias endosimbioticas en agua de consumo de los municipios de PAMPLONA y de VILLA DEL ROSARIO.(Cúcuta: Universidad de Sántander, 2018, 2018-06-25) Jimenez Garza, Marlen Andrea.; Bermon Martinez, Maria Fernanda.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free living amoeba (FLA) are protozoa that are widely distributed in the environment, in fact they are distributed in soils, waters, air conditioners, swimming pools, etc. These microorganisms can develop severe pathologies in humans, mainly due to their various mechanisms of resistance, these pathologies range from courses of serious infections such as primary amoebic meningoencephalitis and granulomatous amebic encephalitis to milder cases such as amoebic keratitis. In reference to the resistance of these microorganisms we have an important factor to highlight as the temperature, since these have a capacity to withstand high temperatures. On the other hand, these microorganisms also have the ability to have an interaction with fungal viruses and bacteria called endosymbiosis, these AVL, in addition to feeding on these microorganisms, give them protection from being digested by them, in addition to external factors and increase in virulence capacity, constituting a Public Health problem. That said, the objective was to determine the presence of free-living amoebas and endosymbiotic bacteria in drinking water in the municipalities of Pamplona and Villa del Rosario.
- PublicaciónRestringidoProtozoos emergentes y oportunistas en agua de consumo de la UNIVERSIDAD DE SANTANDER, CAMPUS CÚCUTA, 2019.(Cúcuta: Universidad de Santander, 2019, 2019-10-22) Farfan Escalante, Marber Julian.; Daza Carozo, Edinson Andrés.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.The protozoa are considered as single-celled organisms which have adapted to different natural habitats, mainly water, largely drinking water that is why the human being must carry out hygienic-sanitary measures that make possible optimal conditions for the same, that is; free of microorganisms and protozoa in question such as Blastocystis spp, free-living amoebas and coccidia, in order to prevent the presence of diseases and infections by the consumption of contaminated water. Therefore it is sought to determine emerging and opportunistic protozoa in water samples of the University of Santander Campus Cúcuta. To achieve this, samples were taken from different drinking water supply points on the university campus, and different laboratory procedures were performed such as filtration, culture media, stains and fresh observations under the microscope in order to observe said microorganisms mentioned above. Based on this of 137 samples analyzed, 78 samples were positive for coccidia, together with more than 65% of the samples positive for Blastocystis spp, therefore, the presence of these microorganisms found indicates the lack of treatments at the different supply points of The institution's water, in turn, would be considered microorganisms that indicate water quality.
- PublicaciónRestringidoRelación de los anticuerpos tipo IGG e IGM contra Toxoplasma Gondii y parásitos intestinales en individuos de la Fundación HOASIS de la Ciudad de CÚCUTA, 2018-2019.(Cúcuta: Universidad de Santander, 2019, 2019-10-23) Jaimes Cobos, Leidy Johana.; Prato Rivera, Sthefanie Gabriela.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Infectious diseases can be caused by parasites, becoming a public health problem in developing countries, affecting millions of people in the world. Toxoplasma gondii is considered one of them being an intracellular protozoan of worldwide distribution, with a very variable virulence, its form of transmission occurs by the ingestion of oocysts which can be in water and food (raw or undercooked meats) from infected animals Transplacental transmission can also occur, by hematological transfusions and organ transplantation. On the other hand, the most common intestinal parasites are divided into two groups (Protozoa and Helminths) and may be present after ingestion of cysts contained in contaminated food and water or due to deficit of hygiene in hands, causing 16 various diseases such as diarrhea, problems of malabsorption, weight loss. This study was developed through a field work with the participation of 61 people from the Hoasis Foundation. Consecutive visits, blood sampling and stool collection were performed.
- PublicaciónRestringidoRelación de los niveles Séricos de TSH con Monoparasitosis por Blastocystis SP en individuos de la Ciudad de CÚCUTA en el 2018-2019.(Cúcuta: Universidad de Santander, 2019, 2019-07-03) Leal Angarita, Natalia.; Meléndez Flórez, Leidy Paola.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Introducción: Blastocystis sp es un parásito cosmopolita que coloniza a millones de personas en todo el mundo. Afecta tanto animales como al ser humano y su vía de transmisión es fecal – oral, es el protozoo que con mayor frecuencia se encuentra en las heces de las personas, tanto sintomáticas como asintomáticas. Este parásito presenta varias controversias e indefiniciones, especialmente en cuanto a su patogenicidad no solo a nivel intestinal sino también extraintestinal, como lo fue en esta investigación. Objetivo: Relacionar los niveles séricos de TSH con monoparasitosis por Blastocystis sp en individuos de la ciudad de Cúcuta en el 2018-2019. Materiales y métodos: Se realizó un cuestionario con preguntas que nos permitió conocer los antecedentes familiares y médicos, los hábitos higiénico-sanitarios y el estilo de vida de cada uno de los participantes. Seguido a esto de procedió a recolectar las heces de estos individuos para realizar el examen directo o coproanálisis y posterior a ello se determinó los niveles séricos de TSH mediante la prueba ELISA. Resultados: Se comprobó que en las muestras analizadas la forma de Blastocystis sp más observada en las heces de los individuos monoparasitados, fueron la forma vacuolar, seguido de la isoforma quística y finalmente la forma ameboide. En cuanto a los niveles de TSH en los individuos que participaron en el estudio, se observó que se presentó un alto grado de significancia en los resultados obtenidos, donde el valor “p” fue < 0.0001, el cual evalúa la relación entre las dos variables. Conclusiones: Se concluyó que si existe una relación entre los niveles séricos de TSH y la monoparasitosis por Blastocystis sp, lo cual permitió comprobar que este parásito si logra desarrollar enfermedades extraintestinales en los individuos infectados.
- PublicaciónRestringidoRelación entre subtipos de Blastocystis SPP. Y Sintomatología intestinal compatible con el Síndrome de Colon irritable en individuos de SAN JOSÉ DE CUCUTA, NORTE DE SANTANDER en el año 2016.(Cúcuta: Universidad de Santander, 2017, 2017-06-24) Molina Pérez, Angie Daniela.; Lizcano López, Rossie Geraldine.; Ríos Ramírez, Yesmit-Karina.; Díaz Carvajal, Claudia-Yaneth.; Contreras Rangel, Jael.Gastrointestinal diseases are one of the main public health problems, especially in developing countries, which do not have good health systems. Blastocystis spp. Is a protozoan with high prevalence worldwide that is transmitted very easily by inadequate hygiene habits and has been related to gastrointestinal symptoms. The main objective of the investigative study was to establish the relationship between subtypes of Blastocystis spp. and irritable bowel syndrome. It is therefore convenient to mention that this syndrome constitutes a gastrointestinal disorder very frequent in medical practice. It is characterized by abdominal pain (or "malaise"), abdominal distension, and is associated with changes in the frequency of bowel movements and / or consistency. In this study we analyzed the symptoms associated with Blastocystis spp. infections compatible with irritable bowel syndrome and the prevalence of the parasite; From a sample of 389 inhabitants of the city of Cúcuta. With regard to symptomatology, diarrhea was the most likely symptom to be associated with the presence of the parasite with a P = 0.0250 value, which is very significant. Coproanalysis was performed and a total of 179 samples were positive for Blastocystis and a total of 44.13% of the samples were monoparasited by Blastocystis spp. Indicating that the samples to discard other enteropathogens were made coprological and modified Ziehl Neelsen staining, and coproculture diarrheal stool and Adenovirus / Rotavirus test, thus obtaining truly monoparasite samples. Conventional PCR was performed on the latter, in order to determine the subtypes of ST1 to ST7 of Blastocystis spp. With ST1 being the most prevalent. It is possible to associate in a great extent the subtype 5 with gastrointestinal symptomatology compatible with the Irritable Intest Syndrome in the population of San José de Cúcuta.