Examinando por Materia "Antimicrobials"

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  • Publicación
    Acceso abierto
    Actividad antibacteriana y citotóxica in vitro de complejos metálicos con ligandos de triazoles
    (Bucaramanga : Universidad de Santander, 2020, 2020-01-20) Barajas Angarita, María Angélica; Sanabria Ayala, Martha Juliana; Roa Cordero, Martha Viviana; Leal Pinto, Sandra-Milena
    The emergence of antimicrobial resistance is currently a public health problem and one of the main challenges of modern medicine that has generated an increase in mortality and morbidity rates associated with infectious pathology. Consequently, WHO published a list of antibiotic-resistant priority pathogens to prioritize research and development of new drugs following the impact these bacteria generate on public health17. Objective. To evaluate the in vitro antimicrobial and cytotoxic activity of the chromium (III) and cobalt (II) metal complexes with triazole ligands in bacterial pathogens and mammalian cells. Methodology. The bioactivity of 6 chromium (III) and cobalt (II) complexes with triazole ligands, 2 inorganic sales and 3 free ligands was studied in reference strains of Staphylococcus aureus (ATTC 11632), Escherichia coli (ATCC10536) and Salmonella enterica serotype Typhimurium (ATCC14028), using the broth microdilution method (CLSI M07-A9). The cytotoxic activity on Vero Cells (CCL-81) was determined by the MTT colorimetric assay. Results: Moderate anti-S aureus activity was demonstrated of two of the three divalent cobalt complexes evaluated (MIC 500 μg mL-1). In general, trivalent chromium complexes and free ligands did not exhibit antibacterial activity (MIC> 2000 μg mL-1). None of the coordination compounds and free ligands studied were cytotoxic for mammalian cells (CC50> 130 μg mL-1). Conclusion: it is established that the chromium complexes and the triazole ligands evaluated do not show significant antibacterial activity. However, there was a moderate anti-S.aureus inhibitory effect produced by two of the trivalent cobalt complexes studied (ID: 4 (diacuadichloro [1,1 '- ((5-methyl-1,3-phenylene) bis ( methylene)) bis (1,2,4-triazole) -N, N '] cobalt (II)), ID: 7 (triacua dichloride [2,6-bis ((1,2,4-triazol-1- ) il) methyl) pyridine-N, N, N '] cobalt (II)), which can be studied in silica form to improve its biological activity. On the other hand, none of the compounds evaluated exhibited a cytotoxic effect against the previously evaluated vero cells.
  • Publicación
    Acceso abierto
    Coinfecciones Respiratorias y Características Sociodemográficas en Pacientes Diagnosticados con COVID-19 en una Clínica Privada de la ciudad de Valledupar 2020-2021
    (Universidad de Santander, 2021-12-15) Tapia Acuña, Dubiela Paola; De La Hoz, Ibis-Margarita
    La enfermedad causada por el nuevo coronavirus COVID-19 fue identificada por primera vez en Wuhan China en diciembre de 2019, es una enfermedad infecciosa causada por el SARS-CoV-2 que afecta principalmente las vías respiratorias. Se ha demostrado que las personas infectadas con COVID-19, presentan un mayor riesgo de coinfecciones, cuando se asocia a neumonía, representa una grave amenaza para los pacientes que cursan con un diagnóstico positivo para COVID-19 y son de alto riesgo aquellos adultos mayores con afecciones preexistentes (2) . Por lo tanto, el objetivo de este trabajo es determinar las coinfecciones respiratorias generadas por bacterias en pacientes diagnosticados con COVID-19 ingresados en la unidad de cuidados intensivos (UCI) en una clínica privada de la ciudad de Valledupar desde marzo 2020 a marzo del 2021. En el presente estudio se recolectaron datos de 315 pacientes diagnosticados con COVID-19 a los cuales se les determino: características sociodemográficas, frecuencia del microorganismo patógeno y perfil antimicrobiano. Los resultados mostraron que el microorganismo más frecuente fue Pseudoma aeroginosa con un 9.94% El rango de edad donde se presentó la mayoría de los casos fue de 51 y 70 años con un 48% y el sexo predominante fue el masculino con un 61%.
  • Publicación
    Restringido
    Evaluación del Efecto Antimicrobiano de Laurus NOBILIS Y THYMUS VULGARIS Frente A Patógenos en Arroz Chino 2019
    (Cúcuta: Universidad de Sántander, 2019, 2019-07-03) Anaya Galvis, Anderson Fabian.; Duran Agudelo, Miguel Humberto.; Martinez Marciales, Karen-Piedad; Contreras Rangel, Jael.
    Chinese rice is a dish that thanks to its high content of nutrients can be an optimal environment for the proliferation of microorganisms. The safety is a very important aspect for the quality of the food, being able to say that it is the guarantee that a food product will not affect the health of the consumer. In the food industry, different products have been developed in order to avoid microbial proliferation and ensure the safety of food. At present the interest of substituting synthetic compounds for natural substances capable of inhibiting microbial proliferation has increased. Taking into account the above, the objective of the present investigation was to evaluate the effect of the essential oils of laurel and thyme in different concentrations, for the control of pathogenic or deteriorating microorganisms of food, with a view to its possible use as a food preservative. In the first instance, the physical-chemical characterization of each essential oil was carried out by means of gas chromatography. Consequent to this, the antimicrobial activity of each essential oil was evaluated; by means of the macrodilution method in broth and the method of diffusion in agar, with the objective of determining the MIC and the CMB of the essential oils of thyme and bay leaf. In the next phase we proceeded to demonstrate the antimicrobial effectiveness of the oils, this time against Chinese rice inoculated intentionally with Staphylococcus aureus and Escherichia coli. In the obtained results it was determined that thyme essential oil has a minimum inhibitory concentration of 92.50 mg / ml against S. aureus and E. coli. On the other hand, laurel essential oil presented an MIC of 413.1 mg / ml against E. Coli but without inhibition against S. aureus, this could be due to the low level of purity of the essential oil of laurel.