Examinando por Materia "Citotoxicidad"

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  • Publicación
    Acceso abierto
    Actividad antimicrobiana de aceites esenciales de Lippia alba y cymbopogon citratus sobre streptococcus mutans y citotoxicidad en células cho
    (2016) Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Mena Álvarez, O.; Martínez Pabón, M.C.; Galvis Pareja, D.; Merini, Luciano J.
    Background: Dental caries is a complex in- fectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary f low, buffering capa- city and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not com- pletely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capabi- lity of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromato- graphy with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mu- tans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium. Results: The major components in both essential oils were Geraniol and Citral. Lippia alba essential oil applied in concen- tration of 0.01 mg / 100 mL removed 95.8% of S. mutans biofilm and C. citratus e s s ent i a l o i l s h o w e d a removal activity of 95.4% in the concentrations 0.1, 0.01mg /100 mL and 93.1% in concentration 0.001 mg / 100 mL. None of the essential oils showed toxicity to CHO cells in a 24-hour treatment, with signif icant differences in relation to the control with methanol (P = 0.00) which inhibits most cells. Conclusions: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biof ilms and null cytotoxicity, evidencing a po- tential use in treating and preventing dental caries.
  • Publicación
    Acceso abierto
    Efecto antibacteriano de una nanoemulsión de ftalocianina de aluminio clorada sobre periodontopatógenos relevantes en el paciente diabético tipo 2: Estudio in vitro
    (Bucaramanga : Universidad de Santander, 2019, 2019-09-16) Lloreda Rey, Laura-Patricia; Herrera Sandoval, Laura-Viviana; Méndez Díaz, Luz-Mery; Leal Pinto, Sandra-Milena
    La enfermedad periodontal (EP) y la diabetes mellitus (DM) son enfermedades crónicas con una relación bidireccional. El tratamiento convencional para la EP es altamente invasivo, consiste en la remoción mecánica de agentes microbianos de las superficies dentales. Investigaciones han propuesto el uso de la terapia fotodinámica (TFD) como modalidad terapéutica prometedora para el manejo de infecciones de la cavidad bucal. En este sentido, el objeto general fue determinar el efecto antimicrobiano in vitro de una nanoemulsión de Ftalocianina de Aluminio Clorada (NE-PcAlCl ) en combinación con la terapia fotodinámica sobre el crecimiento de periodontopatógenos prevalentes en la enfermedad periodontal del paciente diabético tipo 2. Metodológicamente, se evaluó el efecto antibacteriano de NE-PcAlCl y PcAlCl-libre contra Prevotella intermedia, Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans en condiciones de anaerobiosis mediante el método de microdilución. La actividad citotóxica fue evaluada en células Vero por el ensayo colorimétrico usando la sal de tetrazolio MTT. Para la TFD se utilizó una fuente de luz LED a 4.83 julios/cm2 por 2 minutos. Controles sin irradiación fueron probados bajo las mismas condiciones. Los resultados demostraron efecto antibacteriano de la NE-PcAlCl contra P. intermedia con CMI de 0,63 μM luego de la TFD, para el caso de P. gingivalis y A. actinomycetemcomitans no se encontró efecto de la NE-PcAlCl en las concentraciones evaluadas (> 20 μM) antes y después de la TFD. Por otro lado, la PcAlCl-libre y en nanoemulsión mostraron ser fototóxicas sobre células Vero (CC50 de 0,155 y 0,09 μM, respectivamente) sin registrar diferencias significativas entre ellas (p<0.05). En este sentido, los resultados obtenidos sugieren el potencial uso de la TFD en combinación con la NE-PcAlCl para la inhibición de P. intermedia, periodontopatógeno altamente prevalente en el paciente diabético tipo 2.
  • Publicación
    Acceso abierto
    Evaluación in vitro de la actividad antifúngica y citotóxica del eugenol y sus derivados semisintéticos frente a Trichophyton spp
    (Bucaramanga : Universidad de Santander, 2018, 2018-06-26) Rivera Rincón, Yohana; Leal Pinto, Sandra-Milena
    Dermatophytes are keratinophilic fungi that infect tissues such as skin, hair and nails in humans and animals. The treatment is based on topical, systemic and / or combined therapies with low penetrability, varied effectiveness and adverse effects that make it a challenge to research new natural bioactive molecules. The general objective was to evaluate the antifungal and cytotoxic activity in vitro of the semisynthetic compounds derived from eugenol in fungi of the genus Trichophyton. The antifungal susceptibility of twelve eugenol derivatives was evaluated against: Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans. The MIC was determined by the microdilution method established by CLSI, subcultures from MIC, were incubated for 7 days at 30 ° C to determine MFC. In addition, the inhibition of the radial growth of the mycelium was evaluated by the substrate technique impregnated for 20 days. Likewise, in vitro toxicity in Vero cells was tested by a colorimetric assay, using the MTT tetrazolium salt. Terbinafine used as reference drug. EO, eugenol and derivatives 5,10,11 and 12 showed inhibition of T. mentagrophytes MIC 62.5-500 μg / mL and MFC 125-500 μg/mL Eugenol, derivatives 3,5 and 11 inhibited T. rubrum MIC and MFC 125-250 μg/mL EO, eugenol, compound 11 and 12 inhibited T. tonsurans with MIC and MFC 125-250 μg/mL 100% inhibition of mycelial growth of the three dermatophytes was observed after exposure with 300ug/mL and 100 ug/mL of derivatives 3,5,7-12 and 3, 7-9,12, respectively. Low toxicity in Vero cells was observed (CC50 54.06- <300 ug/mL). New bioactive molecules with antifungal effect and low in vitro toxicity are presented, which represents an advance in the pre-clinical process of development and research of new antifungal agents.