Examinando por Materia "Cytotoxicity"
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- PublicaciónAcceso abiertoActividad antimicrobiana de aceites esenciales de Lippia alba y cymbopogon citratus sobre streptococcus mutans y citotoxicidad en células cho(2016) Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Mena Álvarez, O.; Martínez Pabón, M.C.; Galvis Pareja, D.; Merini, Luciano J.Background: Dental caries is a complex in- fectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary f low, buffering capa- city and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not com- pletely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capabi- lity of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromato- graphy with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mu- tans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium. Results: The major components in both essential oils were Geraniol and Citral. Lippia alba essential oil applied in concen- tration of 0.01 mg / 100 mL removed 95.8% of S. mutans biofilm and C. citratus e s s ent i a l o i l s h o w e d a removal activity of 95.4% in the concentrations 0.1, 0.01mg /100 mL and 93.1% in concentration 0.001 mg / 100 mL. None of the essential oils showed toxicity to CHO cells in a 24-hour treatment, with signif icant differences in relation to the control with methanol (P = 0.00) which inhibits most cells. Conclusions: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biof ilms and null cytotoxicity, evidencing a po- tential use in treating and preventing dental caries.
- PublicaciónAcceso abiertoEfecto antibacteriano de una nanoemulsión de ftalocianina de aluminio clorada sobre periodontopatógenos relevantes en el paciente diabético tipo 2: Estudio in vitro(Bucaramanga : Universidad de Santander, 2019, 2019-09-16) Lloreda Rey, Laura-Patricia; Herrera Sandoval, Laura-Viviana; Méndez Díaz, Luz-Mery; Leal Pinto, Sandra-MilenaLa enfermedad periodontal (EP) y la diabetes mellitus (DM) son enfermedades crónicas con una relación bidireccional. El tratamiento convencional para la EP es altamente invasivo, consiste en la remoción mecánica de agentes microbianos de las superficies dentales. Investigaciones han propuesto el uso de la terapia fotodinámica (TFD) como modalidad terapéutica prometedora para el manejo de infecciones de la cavidad bucal. En este sentido, el objeto general fue determinar el efecto antimicrobiano in vitro de una nanoemulsión de Ftalocianina de Aluminio Clorada (NE-PcAlCl ) en combinación con la terapia fotodinámica sobre el crecimiento de periodontopatógenos prevalentes en la enfermedad periodontal del paciente diabético tipo 2. Metodológicamente, se evaluó el efecto antibacteriano de NE-PcAlCl y PcAlCl-libre contra Prevotella intermedia, Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans en condiciones de anaerobiosis mediante el método de microdilución. La actividad citotóxica fue evaluada en células Vero por el ensayo colorimétrico usando la sal de tetrazolio MTT. Para la TFD se utilizó una fuente de luz LED a 4.83 julios/cm2 por 2 minutos. Controles sin irradiación fueron probados bajo las mismas condiciones. Los resultados demostraron efecto antibacteriano de la NE-PcAlCl contra P. intermedia con CMI de 0,63 μM luego de la TFD, para el caso de P. gingivalis y A. actinomycetemcomitans no se encontró efecto de la NE-PcAlCl en las concentraciones evaluadas (> 20 μM) antes y después de la TFD. Por otro lado, la PcAlCl-libre y en nanoemulsión mostraron ser fototóxicas sobre células Vero (CC50 de 0,155 y 0,09 μM, respectivamente) sin registrar diferencias significativas entre ellas (p<0.05). En este sentido, los resultados obtenidos sugieren el potencial uso de la TFD en combinación con la NE-PcAlCl para la inhibición de P. intermedia, periodontopatógeno altamente prevalente en el paciente diabético tipo 2.
- PublicaciónAcceso abiertoEffect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells(2016-10-17) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Galvis Pareja, D.; Jiménez Rios, Hedilka; Merini, Luciano J.; Martínez Pabón, M.C.Background Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Chemical compounds studied in this article List of up to 10 names of chemical compounds studied in the article. Geranial. Neral. Myrcene. Geraniol. (E)-Caryophyllene. trans-Verbenol. Geranyl acetate. cis-Verbenol. Germacrene D. 37-Dimethyl-26-octadiene-1-ol
- PublicaciónAcceso abiertoIn vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.(Mycoses, 2019-01) Leal Pinto, Sandra-Milena; Herrera Sandoval, Laura-Viviana; Vargas Méndez, Leonor-Yamile; CLINIUDESBackground Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells. Methods The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cytotoxic activity. Results MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC50 31.00 ± 0.61 μg/mL. Conclusions Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian cells.
- PublicaciónAcceso abiertoIn vitro susceptibility of Microsporum spp. and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives(2019) Leal Pinto, Sandra Milena; Herrera Sandoval, Laura Viviana; Vargas Méndez, Leonor-YamileBackground: Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary.Objective: To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllusessential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells.Methods: The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthe-sis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhi-bition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cyto-toxic activity.Results: MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC5031.00 ± 0.61 μg/mL.Conclusions: Our study indicates an interesting bioactivity of isoeugenol and meth-ylisoeugenol against M. canis, M. gypseum and mammalian cells.
- PublicaciónAcceso abiertoInhibition of C. albicans dimorphic switch by cobalt(II) complexes with ligands derived from pyrazoles and dinitrobenzoate: Synthesis, characterization and biological activity(2019-07-01) Fonseca, Daniela; Leal-Pinto, Sandra Milena; Roa-Cordero, Martha Viviana; Vargas, Jose D.; Moreno-Moreno, Erika Marcela; Macias, Mario A.; Suescun, Leopoldo; Muñoz-Castro, Alvaro; Hurtado, John J.; MicrobiotaSeven cobalt(II) complexes of pyrazole derivatives and dinitrobenzoate ligands were synthesized and characterized. The single-crystal X-ray diffraction structure was determined for one of the ligands and one of the complexes. The analysis and spectral data showed that all the cobalt complexes had octahedral geometries, which was supported by DFT calculations. The complexes and their free ligands were evaluated against fungal strains of Candida albicans and emerging non-albicans species and epimastigotes of Trypanosoma cruzi. We obtained antifungal activity with a minimum inhibitory concentration (MIC) ranging from 31.3 to 250 µg mL−1. The complexes were more active against C. krusei, showing MIC values between 31.25 and 62.5 µg mL−1 . In addition, some ligands (L1–L6) and complexes (5 and Co(OAc)2 · 4H2O) significantly reduced the yeast to hypha transition of C. albicans at 500 µg mL−1 (inhibition ranging from 30 to 54%). Finally, the complexes and ligands did not present trypanocidal activity and were not toxic to Vero cells. Our results suggest that complexes of cobalt(II) with ligands derived from pyrazoles and dinitrobenzoate may be an attractive alternative for the treatment of diseases caused by fungi, especially because they target one of the most important virulence factors of C. albicans.