Examinando por Materia "Endosymbiosis"
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- PublicaciónRestringidoAcanthamoeba SPP como hospedero de helicobacter pylori en diferentes tipos de agua de SAN JOSE DE CÚCUTA, NORTE DE SANTANDER, 2018-2019(Cúcuta: Universidad de Santander, 2019, 2019-10-22) Bautista, Maria Fernanda.; Espinel Leal, Maria Fernanda.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.The amoebae of free life are cosmopolitan protozoa that are found in humid environments such as soil and water, being the most common that they are found in water sources, whether they are sweet or salty but most prevalent in stagnant waters. Among the free-living amoebas that have a pathogenic capacity for man, those of the genus Acanthamoeba spp and Naegleria spp stand out, and Acanthamoeba spp also has an endosymbiotic role of great relevance with other mainly pathogenic microorganisms. As is Helicobacter pylori which is a gram-negative, spiral-shaped and mobile gram bacillus that is colonizing the stomach of approximately half of the world's population, it has been linked as the causative agent of chronic diseases such as gastritis but in greater relevance with gastric cancer.
- PublicaciónRestringidoDeterminación de vermamoeba vermiformis y bacterias gram negativas patógenas endosimbióticas en muestras de suelo en la CIUDAD DE CÚCUTA.(Cúcuta: Universidad de Sántander, 2018, 2018-12-11) Mogollón Muñoz, Manuel Andrés.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebas are ubiquitous microorganisms, which have been linked to deadly pathologies for humans, and their virulent potential is directly linked to their thermotolerant capacity and the relation of endosymbiosis that can be created with bacteria resistant to amoebic lysis, which It can be fatal to health. The objective was to determine the presence of Vermamoeba vermiformis and endosymbiotic pathogenic Gram negative bacteria in soil samples in the city of Cúcuta. To do this, 3 samples were taken in which soil samples were collected from 5 selected sites (UDES, Comfaoriente College, Juan Atalaya School, Fuente Luminosa and Villasilvania) at strategic points of each site; The samples that were collected were seeded on ANN agar with Page solution and inactivated E. coli. When the samples were analyzed, the amoeba Vermamoeba vermiformis was isolated, after which 4 replicas of the positive samples were made with the amoeba to have a pure culture and to be able to identify Gram-negative bacteria. For the identification of Gramnegative bacteria, lysis was performed on the amoeba using a 27-gauge needle so that bacteria could escape from the interior
- PublicaciónRestringidoEndosimbiosis de Naegleria SPP y Cianobacterias productoras de Microcistinas aisladas de Ambientes hostiles en NORTE DE SANTANDER.(Cúcuta: Universidad de Santander, 2018, 2018-12-11) Villamizar Avellaneda, Génesis Nathaly.; Escalante Riveros, Jessica Johanna.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Naegleria spp. Amoeba of free life characterized presents pathogenic human species, thermophilic properties and capacity to act as endosimbion of other groups of microorganisms, involves microbial relationships and makes possible the survival of the same to hostile conditions. The microorganisms that have been described as resistant to amoebic lysis, are the cyanobacteria, which are prokaryotes that, among other properties, have been characterized by carrying out photosynthesis, but there is a small group of these microorganisms that carry out a alternating metabolism, triggering the release of microcystins such as type D, described as a hepatotoxin with protumoral activity product of organisms such as Microcystis sp., Planktothrix sp. and Anabaena sp. The objective of this research is to establish the endosymbiosis of Naegleria spp. and cyanobacteria producing microcystins isolated from hostile environments in Norte de Santander. The research was based on 4 phases, in 1 place the collection of the samples in the hostile environments (Residual, Thermal and Rivers).
- PublicaciónRestringidoEndosimbiosis entre acanthamoeba spp y cryptococcus spp. En muestras ambientales de la ciudad de CUCUTA, NORTE DE SANTANDER.(Cúcuta: Universidad de Santander, 2018, 2018-12-12) Tarazona Gutierrez, Danna Franchesca.; Vega Galvis, Diana Gabriela.; Contreras Rangel, Jael.; Ríos Ramírez, Yesmit-Karina.Acanthamoeba spp. and Cryptococcus spp. they are opportunistic pathogens, ubiquitous in nature. Many species of Acanthamoeba spp. they are responsible for fatal diseases for humans such as: meningoencephalitis and keratitis, so the study of their endosymbiotic interaction is representative. The purpose of the study was to establish the endosymbiosis between Acanthamoeba spp. and Cryptococcus spp. in environmental samples from the city of Cúcuta, Norte de Santander. A descriptive study was carried out, collecting a total of 132 samples, 120 of soils from four parks in the city of Cúcuta, Norte de Santander: Colón Park, Santander Park, San Eduardo Park, Erasmo Meoz University Hospital Park and 12 water samples taken from the Pamplonita River and the Bogotá Wastewater Canal. The culture was carried out on non-nutritive agar plates previously seeded with inactive Escherichia coli ATCC 25922. Acanthamoeba spp. it was isolated from 83 (70%) of the soil samples and 12 (100%) of the water samples. Also, the identification of Cryptococcus spp. as an endosymbiont, it was present in 7 (5.8%) of the strains samples of Acanthamoeba spp.
- PublicaciónRestringidoIdentificación de tipos de Bacterias Endosimbióticas en Amebas de vida libre aisladas en muestras de suelo del Municipio de TIBÚ, NORTE DE SANTANDER 2018.(Cúcuta: Universidad de Santander, 2019, 2019-07-03) Jauregui Sierra, Karen Tatiana.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free-living amoebas are highly pathogenic microorganisms distributed worldwide, these microorganisms are called amphizoic, since they are capable of living in the environment and act as parasites in humans, belong to the group of Rhizopod protozoa of the order amoebida (Acanthamoeba ) and of the order Schizopyrenida (Naegleria and Balamuthia), are distributed worldwide in various reservoirs such as swimming pool waters, wells, ponds, rivers, water in the air, among others. This group of protozoa produces different pathologies such as primary amebic meningoencephalitis (MAP) by the genus Naegleria; amebic granulomatous encephalitis (GAS) by the genera Acanthamoeba, Balamuthia and Sappinia; which are of clinical relevance since they can compromise the central nervous system; In addition, Acanthamoeba and Balamuthia have been linked to eye injuries such as keratitis and corneal ulcers, as well as primary lesions of the skin. The objective of this study was to identify the interaction of freeflowing amoebas with endosymbiotic bacteria present in soil samples from Tibú Norte de Santander municipality. for this, a series of sampling (3) was carried out in five selected sites within the urban helmet of the municipality, the collected samples were transported in sterile media and were planted in ANN hangars with inactivated E. coli. The samples had a follow-up of 20 days in which a series of peals were made to obtain a pure culture. A quantity of three rings was needed to obtain the pure culture, which was followed by sowing in biochemical media such as the biochemical batteries of citrate, Lía, Tsi and SIM to observe the amoebic endosymbiosis and verify whether the amoeba harbored bacteria, whether Gram negative or Gram positive. Achieving thus isolating amoebas of the genus Acanthamoeba, Naegleria, Balamuthia, Vermamoeba in our work we observed morphology of Gram negative bacteria product of the endosymbiotic mechanism of the genus Acinetobacter and Pseudomonas. As a conclusion, it was possible to observe the presence of amoebas and highly pathogenic bacteria contained in soil samples, which constitutes an important signal in the people of this municipality and public health entities. Recommending taking the necessary measures of prevention to these highly pathogenic microorganisms.
- PublicaciónAcceso abiertoMecanismos fenotípicos de resistencia a antibióticos de bacterias endosimbióticas de amebas de vida libre aisladas de agua de consumo de la Ciudad de CÚCUTA, 2019(Cúcuta, Universidad de Santander, 2020., 2020-10-15) Solano Escalante, Yenny Paola; Cárdenas Ortega, Jessica Liceth; Ríos Ramírez, Yesmit Karina; Contreras Rangel, Jael.Las amebas de vida libre (AVL) son protozoos anfizoicas, heterótrofos, reservorio de virus, hongos y bacterias ya que sobreviven e incluso algunos se multiplican dentro de ellas. Y esto Proporciona un mecanismo de protección para evadir ambientes hostiles generando un aumento en su virulencia, en el caso de las bacterias desarrollan resistencia a los antibióticos; el propósito de este estudio se basa en probar de forma fenotípica los mecanismo de resistencia de bacterias endosimbiòticas aisladas de AVL de agua de consumo. Objetivo: Establecer mecanismos fenotípicos de resistencia a antibióticos de bacterias endosimbiòticas de amebas de vida libre aisladas de agua de consumo de la ciudad de Cúcuta, 2019. Métodos: fueron empleadas técnicas de filtración por membrana y siembra en agar no nutritivo para el aislamiento de AVL, lisis, utilización de batería bioquímica para identificación bacteriana, realización de técnica de difusión en disco para detección fenotípica de mecanismo de resistencia y MicroScan para determinar su sensibilidad. Resultados: se demuestra la presencia de AVL en agua de consumo donde fueron analizadas 30 muestras, distribuidas en tres muestras por comuna, obteniendo un total 12 muestras positivas, con un equivalente de 40% se aislaron bacterias como Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Salmonella Typhi, Rhizobium radiobacter, Ochorobactrum antropi y Staphylococcus auricularis coagulasa negativa. Tanto en la detención de mecanismo de resistencia y sensibilidad frente a los antibióticos BLEE, se identificó en un mayor porcentaje, Conclusiones: se logró identificar la presencia de AVL en diferentes comunas de la ciudad de Cúcuta observando variedad de géneros, entre estos: Acanthamoeba spp, Vermamoeba spp, Vahlkampfia spp; demostrando la alta resistencia de estos protozoos a diferentes procesos de desinfección y el desarrollo de resistencia frente a los antibióticos pueden generar a largo plazo un problema de salud pública.
- PublicaciónRestringidoMycobacterium SPP como endosimbionte de amebas de vida libre en aguas de CÚCUTA, 2018-2019.(Cúcuta: Universidad de Santander, 2019, 2019-10-23) Gomez Garcia, Esteicy Dayanna.; Ramirez Rozo, Maria-Fernanda.Free-living amoebas have been isolated from habitats in common with mycobacteria including cold water for consumption, hot water systems in hospitals, contact lenses, among other habitats. The purpose of this study is to test the endosymbiotic relationship between free-living amoebas and Mycobacterium spp. Objective: Determining endosymbiosis of Mycobacterium spp in free-living amoebas in water samples from Cúcuta, 2019.Methods:The techniques used were membrane filtration and planting in nutritious agar for AVL isolation, lysis and Zielh-Neelsen coloration for the detection of BAAR and confirmation of the presence of these by planting in Ogawa Kudoh agar. Results: the high presence of free-living amoebas in the three samplings in all types of water (49%), indicating that both consumer water, rivers, swimming pool and residuals can possess such microorganisms. there is an endosymbiosis of Mycobacterium spp and aVLs of 30% of the collected samples, confirmed in Zielh Neelsen tying and microbiological culture compatible with Resistant Alcohol Acid Bacillus.
- PublicaciónRestringidoPresencia de amebas de vida libre y bacterias endosimbioticas en agua de consumo de los municipios de PAMPLONA y de VILLA DEL ROSARIO.(Cúcuta: Universidad de Sántander, 2018, 2018-06-25) Jimenez Garza, Marlen Andrea.; Bermon Martinez, Maria Fernanda.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.Free living amoeba (FLA) are protozoa that are widely distributed in the environment, in fact they are distributed in soils, waters, air conditioners, swimming pools, etc. These microorganisms can develop severe pathologies in humans, mainly due to their various mechanisms of resistance, these pathologies range from courses of serious infections such as primary amoebic meningoencephalitis and granulomatous amebic encephalitis to milder cases such as amoebic keratitis. In reference to the resistance of these microorganisms we have an important factor to highlight as the temperature, since these have a capacity to withstand high temperatures. On the other hand, these microorganisms also have the ability to have an interaction with fungal viruses and bacteria called endosymbiosis, these AVL, in addition to feeding on these microorganisms, give them protection from being digested by them, in addition to external factors and increase in virulence capacity, constituting a Public Health problem. That said, the objective was to determine the presence of free-living amoebas and endosymbiotic bacteria in drinking water in the municipalities of Pamplona and Villa del Rosario.