Examinando por Materia "Trypanosoma cruzi"
Mostrando 1 - 10 de 10
Resultados por página
Opciones de clasificación
- PublicaciónAcceso abiertoCardioprotección por antioxidantes mitocondriales en cardiomiocitos humanos infectados con Trypanosoma cruzi(Bucaramanga : Universidad de Santander, 2017, 2017-10-15) Rodríguez Avila, Yudy A.; López Casillas, MarcosLa cardiomiopatía chagásica es el evento más serio y más frecuente de la enfermedad de chagas. La invasión por T. cruzi genera alteraciones en la función mitocondrial del cardiomiocito que se traducen a pérdida del potencial de membrana mitocondrial, generación de especies reactivas de oxígeno ROS y alteración en el balance antioxidante, contribuyendo a un desequilibrio metabólico y oxidativo en el miocardio chagásico. Nosotros evaluamos los efectos de nitróxidos miméticos de SOD (SG1 y Mito-SG1) y del tratamiento de referencia BZN sobre la capacidad pro-oxidante, la bioenergética celular e infectividad de cardiomiocitos humanos AC16 infectados con T. cruzi. Materiales y Métodos: La citotoxicidad celular fue realizada por el ensayo MTT. La infectividad parasitaria fue determinada por citometría de flujo, utilizando el reactivo CFSE. La bioenergética celular se determinó con el sistema Seahorse XFe-24 Analyzer y la producción de ROS Mitocondrial por MitoSOX™ Red. Resultados: El CC50 para SG1 fue de 4790 μM, para Mito-SG1 de 5,0 μM. BZN y Mito-SG1 tuvieron efectos en la disminución de la tasa de infectividad de los cardiomiocitos humanos. Se obtuvo diferencias significativas en los efectos pro-oxidantes de los cardiomiocitos tratados con BZN. Se observaron cambios importantes estadísticamente en los parámetros de respiración basal, producción de ATP, protón leak y respiración máxima en los cardiomiocitos infectados con T. cruzi. La capacidad de reserva respiratoria disminuyo en los cardiomiocitos infectados y sometidos a los tratamientos con BZN, SG1 y Mito-SG1. Conclusiones: El cardiomiocito humano sufre perturbaciones metabólicas tempranas que involucran cambios en el consumo de oxígeno y perfil bioenergético hacia la utilización de la vía glucolítica. La disminución de la infectividad en los cardiomiocitos tratados con Mito-SG1, es un hallazgo que permite realizar estudios futuros con el fin de determinar posibles efectos antiparásitarios del mitocompuesto.
- PublicaciónAcceso abiertoDisagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region(2021-05-03) Torcoroma-García, Liliana; Rocío Aguilar, Jhancy; Bueno, Marly Yojhana; Moreno, Erika Marcela; Ramírez, Herminia; Daza, Nelson; CliniUDESIntroduction:Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologicand molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander. Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott) , and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™. Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR. Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.
- PublicaciónRestringidoFactores de riesgo de infección con Trypanosoma Cruzi en la Localidad de SAN JERONIMO, CÚCUTA, NORTE DE SANTANDER.(Cúcuta: Universidad de Santander, 2019, 2019-07-04) Torres Ochoa, Adriana Carolina.; Gutiérrez Marín, Reinaldo.; Contreras Rangel, Jael.Chagas disease or American trypanosomiasis, named after its discoverer Carlos Justiniano Ribeiro das Chagas, is a life-threatening threat for a quarter of the population located from the southern United States to Patagonia in Argentina, presenting variability from one endemic area to another in its clinical manifestations and epidemiological characteristics. The causative agent is the protozoan Trypanosoma cruzi (T.cruzi), characterized by circulating in nature in a large number of vertebrate, wild and domestic reservoirs, dozens of species of insect vectors and eventually in humans. The intrusion of human populations into natural transmission niches or rock formations that serve as a nest for the reservoirs and these to the colonies of the vector insects, the precarious construction of the houses, the ignorance of the disease and its mode of transmission, are relevant risk factors so that people take the necessary measures and thus avoid a possible infection with the parasite. The objective of this work was to determine the infection risk factors with Trypanosoma cruzi in the locality of San Jerónimo, Cúcuta, Norte de Santander, identifying the entomological indexes and the characteristics of the present dwellings. To achieve the objective, the household survey knowledge was applied. , attitudes and practices on risk factors for Chagas and an active search for triatomine sampling was made in the homes, the triatomines collected were duly identified by means of the key of Lent and Wygodzinsky, such as Eratyrus mucronatus, to establish the infection with Trypanosoma sp, intestinal content was examined by two methods. Concluding from this work that there are risk factors associated with Chagas disease, which make people in the locality vulnerable to acquire a possible infection in the future with this parasite.
- PublicaciónAcceso abiertoImmunomodulation and antioxidant activities aspossible trypanocidal and cardioprotectivemechanisms of major terpenes from lippia albaessential oils in an experimental model of chronicchagas disease(2021-11-22) Espinel-Mesa, Denerieth Ximena; González Rugeles, Clara Isabel; Mantilla Hernández, Julio César; Stashenko, Elena E.; Villegas-Lanau, Carlos Andrés; Quimbaya Ramírez, John-Jaime; Torcoroma-García, Liliana; MasiraIn the late phase of Trypanosoma cruzi infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect only the elimination of the parasite, but are ineffective for late stage treatment and for preventing heart damage and disease progression. In vivo trypanocidal and cardioprotective activity has been reported for Lippia alba essential oils (EOs), ascribed to their two major terpenes, limonene and caryophyllene oxide. To investigate the role of antioxidant and immunomodulatory mechanisms behind these properties, chronic-T. cruzi-infected rats were treated with oral synergistic mixtures of the aforementioned EOs. For this purpose, the EOs were optimized through limonene-enrichment fractioning and by the addition of exogenous caryophyllene oxide (LIMOX) and used alone or in combined therapy with subtherapeutic doses of BNZ (LIMOXBNZ). Clinical, toxicity, inflammatory, oxidative, and parasitological (qPCR) parameters were assessed in cardiac tissue. These therapies demonstrated meaningful antioxidant and immunomodulatory activity on markers involved in CCC pathogenesis (IFN-γ, TNF-α, IL-4, IL-10, and iNOS), which could explain their significant trypanocidal properties and their noteworthy role in preventing, and even reversing, the progression of cardiac damage in chronic Chagas disease.
- PublicaciónAcceso abiertoImmunomodulatory, trypanocide, and antioxidant properties of essential oil fractions of Lippia alba (Verbenaceae)(2021-07-02) Quintero, Wendy Lorena; Moreno, Erika Marcela; Leal Pinto, Sandra Milena; Sanabria, Sandra Milena; Stashenko, Elena; García, Liliana Torcoroma; CliniUDESBackground: Parasite persistence, exacerbated and sustained immune response, and continuous oxidative stress have been described to contribute to the development of the cardiac manifestations in Chronic Chagas Disease. Nevertheless, there are no efficient therapies to resolve the Trypanosoma cruzi infection and prevent the disease progression. Interestingly, trypanocide, antioxidant, and immunodulatory properties have been reported separately for some major terpenes, as citral (neral plus geranial), limonene, and caryophyllene oxide, presents in essential oils (EO) extracted from two chemotypes (Citral and Carvone) of Lippia alba. The aim of this study was to obtain L. alba essential oil fractions enriched with the aforementioned bioactive terpenes and to evaluate the impact of these therapies on trypanocide, oxidative stress, mitochondrial bioenergetics, genotoxicity, and inflammatory markers on T. cruzi-infected macrophages. Methods: T. cruzi-infected J774A.1 macrophage were treated with limonene-enriched (ACT1) and citral/caryophyllene oxide-enriched (ACT2) essential oils fractions derived from Carvone and Citral-L. alba chemotypes, respectively. Results: ACT1 (IC50 = 45 ± 1.7 μg/mL) and ACT2 (IC50 = 80 ± 1.9 μg/mL) exhibit similar trypanocidal effects to Benznidazole (BZN) (IC50 = 48 ± 2.5 μg/mL), against amastigotes. Synergistic antiparasitic activity was observed when ACT1 was combined with BZN (∑FIC = 0.52 ± 0.13 μg/mL) or ACT2 (∑FIC = 0.46 ± 1.7 μg/mL). ACT1 also decreased the oxidative stress, mitochondrial metabolism, and genotoxicity of the therapies. The ACT1 + ACT2 and ACT1 + BZN experimental treatments reduced the pro-inflammatory cytokines (IFN-γ, IL-2, and TNF-α) and increased the antiinflammatory cytokines (IL-4 and IL-10). Conclusion: Due to its highly trypanocidal and immunomodulatory properties, ACT1 (whether alone or in combination with BZN or ACT2) represents a promising L. alba essential oil fraction for further studies in drug development towards the Chagas disease control.
- PublicaciónAcceso abiertoInduction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)(2018-07-27) Moreno Moreno, Erika Marcela; Leal Pinto, Sandra Milena; Stashenko, Elena E.; García Sánchez, Liliana TorcoromaBackground Chagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease’s progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal plants as Lippia alba, offer a promising reserve of bioactive molecules. The principal goal of this work is to characterize the inhibitory properties and cellular effects of the Citral and Carvone L. alba chemotype essential oils (EOs) and their main bioactive terpenes (and the synergies among them) on T. cruzi forms. Methods Twelve L. alba EOs, produced under diverse environmental conditions, were extracted by microwave assisted hydrodistillation, and chemically characterized using gas chromatography coupled mass spectrometry. Trypanocidal activity and cytotoxicity were determined for each oil, and their major compounds, on epimastigotes (Epi), trypomastigotes (Tryp), amastigotes (Amas), and Vero cells. Pharmacologic interactions were defined by a matrix of combinations among the most trypanocidal terpenes (limonene, carvone; citral and caryophyllene oxide). The treated cell phenotype was assessed by fluorescent and optic microscopy, flow cytometry, and DNA electrophoresis assays. Results The L. alba EOs displayed significant differences in their chemical composition and trypanocidal performance (p = 0.0001). Citral chemotype oils were more trypanocidal than Carvone EOs, with Inhibitory Concentration 50 (IC50) of 14 ± 1.5 μg/mL, 22 ± 1.4 μg/mL and 74 ± 4.4 μg/mL, on Epi, Tryp and Amas, respectively. Limonene exhibited synergistic interaction with citral, caryophyllene oxide and Benznidazole (decreasing by 17 times its IC50) and was the most effective and selective treatment. The cellular analysis suggested that these oils or their bioactive terpenes (citral, caryophyllene oxide and limonene) could be inducing T. cruzi cell death by an apoptotic-like mechanism. Conclusions EOs extracted from L. alba Citral chemotype demonstrated significant trypanocidal activity on the three forms of T. cruzi studied, and their composition and trypanocidal performance were influenced by production parameters. Citral, caryophyllene oxide, and limonene showed a possible induction of an apoptotic-like phenotype. The best selective anti-T. cruzi activity was achieved by limonene, the effects of which were also synergic with citral, caryophyllene oxide and benznidazole.
- PublicaciónAcceso abiertoInhibition of C. albicans dimorphic switch by cobalt(II) complexes with ligands derived from pyrazoles and dinitrobenzoate: Synthesis, characterization and biological activity(2019-07-01) Fonseca, Daniela; Leal-Pinto, Sandra Milena; Roa-Cordero, Martha Viviana; Vargas, Jose D.; Moreno-Moreno, Erika Marcela; Macias, Mario A.; Suescun, Leopoldo; Muñoz-Castro, Alvaro; Hurtado, John J.; MicrobiotaSeven cobalt(II) complexes of pyrazole derivatives and dinitrobenzoate ligands were synthesized and characterized. The single-crystal X-ray diffraction structure was determined for one of the ligands and one of the complexes. The analysis and spectral data showed that all the cobalt complexes had octahedral geometries, which was supported by DFT calculations. The complexes and their free ligands were evaluated against fungal strains of Candida albicans and emerging non-albicans species and epimastigotes of Trypanosoma cruzi. We obtained antifungal activity with a minimum inhibitory concentration (MIC) ranging from 31.3 to 250 µg mL−1. The complexes were more active against C. krusei, showing MIC values between 31.25 and 62.5 µg mL−1 . In addition, some ligands (L1–L6) and complexes (5 and Co(OAc)2 · 4H2O) significantly reduced the yeast to hypha transition of C. albicans at 500 µg mL−1 (inhibition ranging from 30 to 54%). Finally, the complexes and ligands did not present trypanocidal activity and were not toxic to Vero cells. Our results suggest that complexes of cobalt(II) with ligands derived from pyrazoles and dinitrobenzoate may be an attractive alternative for the treatment of diseases caused by fungi, especially because they target one of the most important virulence factors of C. albicans.
- PublicaciónAcceso abiertoSeroprevalencia de Anticuerpos Anti Trypanosoma cruzi en Donantes de un Banco de Sangre de Valledupar 2019 - 2020(Universidad de Santander, 2021-12-06) Angulo-Puello, Ana Lucia; Jiménez-Pacheco, Angie Lorena; Fragoso-Amaya, Elia Mercedes; Martínez-Pedroza, Daira
- PublicaciónAcceso abiertoSeroprevalencia de Anticuerpos ContraTrypanosoma cruzien Caninos del Área Metropolitana de Bucaramanga(2020-05-29) Vanegas Jerez, Daniel Felipe; Silva Gómez, Diego Camilo Alejandro; Quimbaya Ramírez, John-JaimeLa enfermedad de Chagas es una zoonosis ocasionada por el Trypanosoma cruzi. Protozoario que parasita más de 150 especies de mamíferos. Es una enfermedad desatendida que afecta a millones de personas en el mundo. En Colombia se cree que cerca de 7’000.000 se encuentran infectadas. Santander es un departamento endémico. El perro doméstico (Canis lupus familiaris) es susceptible y puede infectarse por vía vectorial, congénita, transfusional y oral. No obstante, la infección presenta cuadros inespecíficos, siendo divisible para los médicos veterinarios. La OPS considera que los perros domésticos son efectivos centinelas de la infección para la vigilancia de La enfermedad de Chagas, y en vista de la necesidad de visualizar la problemática veterinaria y establecer la proporción de infecciones por T. cruzi en el AMB. En octubre de 2018 y Julio de 2019 se tomaron muestras sanguíneas en 530 perros domésticos con propietario reconocible que habitaban en 15 áreas representativas del AMB. El serodiagnóstico se realizó mediante ELISA y el punto de corte para las densidades ópticas se estableció con base en el promedio de controles negativos más dos desviaciones estándar. Se observó seroprevalencia en 17.1% (90/526) de los perros. Bucaramanga presentó mayor prevalencia (21%), seguido de Girón (18.3%), Piedecuesta (12.6%) y Floridablanca (10.6%). Las áreas con mayor prevalencia se ubicaron al occidente de Bucaramanga (38.7%) y al nororiente de Giró (63.1%), La menor prevalencia se observó en áreas al occidente de Girón (0%), oriente de Piedecuesta (2.5%), oriente (9.5%) y suroccidente (2.9%) de Floridablanca y en el centro de Bucaramanga (7.3%).
- PublicaciónAcceso abiertoTrypanosoma cruzi : Prevalencia y factores de riesgo de seropositividad en donantes de sangre del Hemocentro y unidad de aféresis, Valledupar, Colombia, 2013-2014(2014-12-19) Rocha-Muñoz, Linda P.; Hernández Peñaranda, Indira Paola; Martínez Pedraza, Mairta M.; Castellanos Domínguez, Yeny ZulayIntroducción: La transmisión de Trypanosoma cruzi por transfusiones sanguíneas representa la segunda línea de infección después de la vectorial, lo que hace necesario identificar algunas particularidades socioepidemiológicas en los donantes que permitan predecir la infección por Trypanosoma cruzi. objetivo: Determinar la prevalencia de anticuerpos anti-Trypanosoma cruzi y los factores de riesgo de seropositividad en donantes de sangre voluntarios del Hemocentro y Unidad de Aféresis de Valledupar, Colombia. Materiales y métodos: Se realizó un estudio descriptivo de corte transversal en 170 donantes de sangre voluntarios reclutados entre 2013 y 2014. La detección de anticuerpos IgG anti-Trypanosoma cruzi se realizó mediante pruebas serológicas. Las variables que predicen la seropositividad para Trypanosoma cruzi se indagaron mediante un cuestionario socioepidemiológico y un posterior análisis bivariado y de regresión logística. Resultados: Se encontró una prevalencia general de infección por Trypanosoma cruzi del 0,14%. Las variables que permitieron predecir la seropositividad en los donantes fueron: la presencia del vector en la casa, el haber sido picado por el vector alguna vez en la vida y habitar en una casa de tapia pisada o bajareque durante la infancia. Conclusiones: Aunque la prevalencia de anticuerpos anti-Trypanosoma cruzi fue baja en los donantes de sangre de Valledupar, Colombia, este estudio revela la importancia de incorporar preguntas en el formulario de selección de donantes que adviertan al profesional del banco de sangre sobre una posible infección con el parásito, para así optimizar el tamizaje de unidades de sangre y su clasificación como no aptas para transfusión.