Publicación:
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrix

Página de artículo simple
dc.contributor.authorRopero-Vega, Jose Luis
dc.contributor.authorRedondo-Ortega, Joshua Felipe
dc.contributor.authorGalvis-Curubo, Yuli Juliana
dc.contributor.authorRondon-Villarreal, Paola
dc.contributor.authorFlorez-Castillo, Johanna Marcela
dc.contributor.researchgroupCibasspa
dc.date.accessioned2022-02-25T16:56:17Z
dc.date.available2022-02-25T16:56:17Z
dc.date.issued2021-04-28
dc.descriptionDigitalspa
dc.description.abstractCurrently, the detection of pathogens such as Escherichia coli through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties such as simplicity, portable, affordable, user‑friendly, and deliverable to end‑users. For this, in this work we report for the first time, to our knowledge, the bioinformatic design of a new peptide based on TIR protein, a receptor of Intimin membrane protein which is characteristic of E. coli. This peptide (named PEPTIR‑1.0) was used as recognition element in a biosensor based on AuNPs‑modified screen‑printed electrodes for the detection of E. coli. The morphological and electrochemical characteristics of the biosensor obtained were studied. Results show that the biosensor can detect the bacteria with limits of detection and quantification of 2 and 6 CFU/mL, respectively. Moreover, the selectivity of the system is statistically significant towards the detection of the pathogen in the presence of other microorganisms such as P. aeruginosa and S. aureus. This makes this new PEPTIR‑1.0 based biosensor can be used in the rapid, sensitive, and selective detection of E. coli in aqueous matrices.spa
dc.description.researchareaCiencias Exactas y Naturalesspa
dc.format.extent17 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.3390/molecules26092559
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/6167
dc.language.isoengspa
dc.publisherSulka, Grzegorz D.spa
dc.publisherBogdanowicz, Robertspa
dc.publisher.placeSuizaspa
dc.relation.citationendpage17spa
dc.relation.citationissue2559spa
dc.relation.citationstartpage1spa
dc.relation.citationvolume26spa
dc.relation.citesRopero-Vega, J.L.; Redondo-Ortega, J.F.; Galvis-Curubo, Y.J.; Rondón-Villarreal, P.; Flórez-Castillo, J.M. A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of E. coli O157:H7 in an Aqueous Matrix. Molecules 2021, 26, 2559. https:// doi.org/10.3390/molecules26092559
dc.relation.indexedScopusspa
dc.relation.ispartofjournalMoleculesspa
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerlandspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución 4.0 Internacional (CC BY 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcehttps://www.mdpi.com/1420-3049/26/9/2559spa
dc.subject.proposalPathogeneng
dc.subject.proposalPEPTIR-1.0eng
dc.subject.proposalScreen-printed electrodeseng
dc.subject.proposalGold nanoparticleseng
dc.subject.proposalElectrodepositioneng
dc.subject.proposalLimit of detectioneng
dc.subject.proposalSelectivityeng
dc.titleA bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157:H7 in an aqueous matrixspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
Archivos
Paquete original
Mostrando 1 - 1 de 1
Imagen en miniatura
Nombre:
A bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of e. Coli o157H7 in an aqueous matrix.pdf
Tamaño:
216.57 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Paquete de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
59 B
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
AGCBA. Artículos de Investigación