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Anti-Candida albicans y Antiinflamatorio In vitro de Mezclas Optimizadas de Lippia alba Aplicadas Sobre Membranas de Regeneración Ósea Guiada (ROG)

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dc.contributor.advisorGarcía-Sánchez, Liliana Torcoroma
dc.contributor.advisorQuintero-García, Wendy Lorena
dc.contributor.authorRugeles-Páez, Nohora Camila
dc.contributor.juryPuerta-Arias, Juan David
dc.contributor.jurySánchez-Villamil, Juana Patricia
dc.date.accessioned2022-11-28T16:15:08Z
dc.date.available2024-10-25
dc.date.available2022-11-28T16:15:08Z
dc.date.issued2022-10-24
dc.descriptionDigital
dc.description.abstractLa Regeneración Ósea Guiada (ROG) es una técnica de aumento de reborde que a menudo requiere el uso de membranas. Estas actúan como barrera física facilitando la compartimentación de células con potencial de regeneración y excluyendo células epiteliales. La falta de propiedades osteoconductoras, la inflamación y la infección son las limitaciones más frecuentes en ROG, que requieren terapias de apoyo con biocidas, antibióticos y antiinflamatorios, que pueden promover la disbiosis y la resistencia a los antimicrobianos. Para optimizar las propiedades inmunorresistentes y antifúngicas de las membranas de colágeno, el objetivo de este trabajo fue estudiar aditivos profilácticos basados en mezclas sinérgicas de clorhexidina con fracciones enriquecidas de aceites esenciales de Lippia alba. Las combinaciones del biocida con fracciones ricas en citral y limoneno demostraron aumentar de dos a ocho veces, respectivamente, el rendimiento anti-Candida albicans de la clorhexidina, en términos de concentración inhibitoria mínima. Como pretratamientos en membranas, estas mezclas potenciaron hasta cuatro veces la acción inhibidora del crecimiento de C. albicans de la clorhexidina, en superficies. En células de macrófago murino J774A.1, se evidenció una interacción farmacológica antagónica (citoprotector) entre las fitoterapias y la clorhexidina, provocando un aumento al doble de la Concentración Citotóxica 50 biocida (CC50) y minimizando sus efectos tóxicos sobre la adhesión celular, membrana celular, potencial mitocondrial y la integridad nuclear. En los macrófagos, las fracciones enriquecidas con citral también favorecen las propiedades de respuesta inmunitaria de las membranas de colágeno (solas o en combinación con clorhexidina) aumentando la citoquina TNF-α al mismo tiempo que aumenta IL-4. Así, esta terapia combinada representa una plataforma prometedora para el desarrollo de una solución biocida profiláctica o terapéutica capaz de optimizar las características farmacológicas de la clorhexidina, tanto en su tolerancia epitelial como en su acción para prevenir la consolidación de C. albicans en la superficie de la membrana.spa
dc.description.abstractIn dental rehabilitation with implants, Guided Bone Regeneration (GBR) is a ridge technique often required for successful bone augmentation through membranes. These devices act a physical barrier facilitating the compartmentalization of cells with potential in osseous regeneration and excluding cells impeding bone formation. Lack of osteo-conducting properties, inflammation, and infection are the most often limitations in GBR, requiring biocidal, antibiotic, and anti-inflammatory support therapies, which can promote dysbiosis and antimicrobial resistance. To optimize the immune-responsive and antifungal properties of GBR-resorbable membranes (collagen), the aim of this work was to study prophylactic additives based in synergistic mixtures of chlorhexidine with terpene-enriched fractions from Lippia alba essential oils. Combinations of the biocide with citral- and limonene-rich fractions showed to increase from twice to eight times, respectively, the anti-Candida albicans performance of chlorhexidine, in terms of minimum inhibitory concentration. As pretreatments on membranes, these mixtures potentiated up to four times the C. albicans-growth inhibitory action of chlorhexidine, on surfaces. On murine macrophage J774A.1 cells, an antagonistic pharmacologic interaction (cytoprotective) was evidenced between phytotherapies and chlorhexidine, causing an increase in twice of the biocidal Cytotoxic Concentration 50 (CC50) and minimizing its toxic effects on cell adhesion, cell membrane, mitochondrial potential, and nuclear integrity. On macrophages, citral-enriched fractions also favor the immune-responsive properties of collagen membranes (alone or in combination with chlorhexidine) increasing the osteogenic TNF-α cytokine while increasing the inhibitory IL-4. Thus, this combined therapy represents a promising platform for the development of a prophylactic (attached to membranes) or therapeutic biocidal solution able of optimizing the pharmacological characteristics of chlorhexidine, both in its epithelium tolerance and in its action to prevent C. albicans consolidation on the membrane surface. Due to their ability to stimulate a regulated inflammatory response, this therapy may also contribute to the role of collagen membranes as bone healing promoting agents.eng
dc.description.degreelevelMaestría
dc.description.degreenameMagíster en Investigación en enfermedades Infecciosas
dc.description.tableofcontentsIntroducción ............................................................................................................................... 20 Marco Referencial ................................................................................................................... 22 Regeneración Ósea Guiada (ROG) ............................................................................................... 22 Complicaciones del Uso de Membranas en ROG......................................................................... 24 Actividad de los Macrófagos en la ROG. ..................................................................................... 25 Plantas Medicinales de Uso Odontológico ................................................................................... 28 Lippia alba. ................................................................................................................................... 29 Quimiotipos. .................................................................................................................................. 30 Limoneno....................................................................................................................................... 33 Citral. ........................................................................................................................................ 34 Óxido Cariofileno. ........................................................................................................................ 34 Clorhexidina .................................................................................................................................. 34 Actividad Antimicrobiana. ............................................................................................................ 35 Efecto Secundarios y Contraindicaciones .................................................................................... 35 Combinaciones de la Clorhexidina con Otros Compuestos. ........................................................ 36 Objetivos ................................................................................................................... 38 Objetivo General ........................................................................................................................... 38 Objetivos Específicos.................................................................................................................... 38 Materiales y Métodos ................................................................................................................... 39 Materiales ...................................................................................................................................... 39 Material Vegetal y Fracciones Enriquecidas de Aceites Esenciales Derivadas de L. alba. ........ 39 Terpenos. ....................................................................................................................................... 40 Clorhexidina. ................................................................................................................................ 40 Membranas de ROG. .................................................................................................................... 40 Línea Celular. ............................................................................................................................... 41 Línea Fúngica. .............................................................................................................................. 41 Métodos ........................................................................................................................................ 41 Composición Química Relativa de los Aceites Esenciales y de sus Fracciones Derivadas de L. alba. ........................................................................................................................................ 41 Evaluación de la Actividad Antifúngica. ...................................................................................... 42 Evaluación de la Actividad Citotóxica. ........................................................................................ 43 Interacciones Farmacológicas Sobre Macrófagos Murinos J774A.1 y C. albicans. ................... 44 Cambios Morfológicos de Macrófagos Murinos J774A.1. ........................................................... 45 Evaluación del Estrés Oxidativo. .................................................................................................. 46 Evaluación de Citoquinas. ............................................................................................................ 46 Ensayo Anti-Proliferativo Sobre Membranas de Colágeno con C. albicans. .............................. 47 Análisis de Datos. ......................................................................................................................... 48 Resultados ................................................................................................................... 49 Composición Química de Fracciones Enriquecidas de Lippia alba ............................................. 49 Curva Crecimiento Candida albicans ........................................................................................... 50 Actividad Antifúngica ................................................................................................................... 51 Actividad Citotóxica ..................................................................................................................... 53 Interacciones Farmacológicas Sobre Macrófagos Murinos J774A.1 y C. albicans ..................... 54 Actividad Anti-Proliferativa Contra C. albicans en Membranas de Colágeno Pre-Tratadas ....... 59 Cambios Morfológicos de Macrófagos y Estrés Oxidativo Sobre Macrófagos Murinos J774A.1 .. ........................................................................................................................................ 61 Evaluación de Citoquinas ............................................................................................................. 65 Discusión ............................................................................................................................. 71 Conclusiones ............................................................................................................................. 88 Recomendaciones ........................................................................................................................ 89 Referencias Bibliográficas ............................................................................................................ 90
dc.format.extent110 p
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad de Santander
dc.identifier.localT 86.22 R832e
dc.identifier.reponameRepositorio Digital Universidad de Santander
dc.identifier.repourlhttps://repositorio.udes.edu.co/
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/7843
dc.language.isospa
dc.publisherUniversidad de Santander
dc.publisher.facultyFacultad Ciencias Médicas y de la Salud
dc.publisher.placeBucaramanga, Colombia
dc.publisher.programMaestría en Investigación en enfermedades Infecciosas
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dc.rightsDerechos Reservados - Universidad de Santander, 2022
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_14cb
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.proposalRegeneración ósea guiadaspa
dc.subject.proposalInmune-respuestaspa
dc.subject.proposalCandida albicansother
dc.subject.proposalClorhexidinaother
dc.subject.proposalLippia albaother
dc.subject.proposalGuided bone regenerationeng
dc.subject.proposalImmuno-responsiveeng
dc.titleAnti-Candida albicans y Antiinflamatorio In vitro de Mezclas Optimizadas de Lippia alba Aplicadas Sobre Membranas de Regeneración Ósea Guiada (ROG)spa
dc.title.translatedAnti-Candida albicans and Anti-Inflammatory Effect in vitro of Optimized Mixtures of Lippia alba Applied on Guided Bone Regeneration Membranes (GBR)
dc.typeTrabajo de grado - Maestría
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/submittedVersion
dcterms.audienceTodas las Audienciasspa
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