Please use this identifier to cite or link to this item: http://repositorio.udes.edu.co/handle/001/1069
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSandoval Pabón, Ludwing-
dc.date.accessioned2019-02-08T15:13:33Z-
dc.date.available2019-02-08T15:13:33Z-
dc.date.issued2018-12-14-
dc.identifier.urihttp://repositorio.udes.edu.co/handle/001/1069-
dc.description48 p. Cdspa
dc.description.abstractEl hierro es uno de los elementos más abundante en la Tierra y el metal más cuantioso en el cuerpo humano. Este elemento es decisivo para el desarrollo de la vida porque casi todos los organismos necesitan hierro para realizar diferentes actividades biológicas. En los humanos la concentración de Fe está regulada a través de varias proteínas que contienen este elemento, como la transferrina (TF). La TF transporta Fe a cada célula que lo necesita y lo mantiene alejado de los patógenos. Estos organismos han desarrollado diferentes estrategias para la obtención de hierro. Uno de ellos es la expresión de receptores de transferrina específicos localizados en la membrana plasmática. Es el caso de Entamoeba histolytica, microrganismo que requiere de Fe para sobrevivir y en el que se ha observado que una vez que la TF se une a su receptor las vías de transducción de señales relacionadas con la activación de este tienen importancia en la proliferación. El estudio de los receptores de transferrina y otras proteínas relacionadas con las vías de señalización es importante porque estas proteínas podrían usarse como dianas terapéuticas debido a su especificidad. En este informe, se describe tanto el cultivo como la obtención de proteínas que presentan un estado de fosforilación por la utilización de la TF en E.histolytica. El alcance esperado del presente trabajo es contribuir al estudio del uso de la transferina mediante la identificación de las proteínas que participan en las vías de señalización y que son útiles como objetivos terapéuticos.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherBucaramanga : Universidad de Santander, 2018spa
dc.rightsDerechos Reservados - Universidad de Santander, 2018spa
dc.titleCultivo de entamoeba histolytica y obtención de proteínas fosforiladas por la acción de transferrina en este parásitospa
dc.typeTrabajo de grado - Pregradospa
dcterms.bibliographicCitationAGUILAR DIAZ, Hugo. CARREÑO, Julio. ARGUELLO, Raul. LACLETTE, Juan Pedro y MORALES MONTOR, Jorge. (2011). Cyst and encystment in protozoan parasites: optimal targets for new life-cycle interrupting strategies?https://doi.org/10.1016/j.pt.2011.06.003-
dcterms.bibliographicCitationAVILA, Eva. MARTINEZ, Edith. SABANERO, Gloria. BARON, Elda. NEFRETTE, Sergio y SANDOVAL, Roberto..(2002).Subcellular localization of the nad+-dependent alcohol dehydrogenase in entamoeba histolytica trophozoites . Journal of Parasitology 2002 88 (2), DOI: 10.1645/00223395(2002)088[0217:SLOTND]2.0.CO;2-
dcterms.bibliographicCitationBEGUM, Sharmin. QUACH, Jeanie y CHADEE, Kris. (2015). Immune Evasion Mechanisms of Entamoeba histolytica: Progression to Disease.Front Microbiol. 2015 Dec15;6:1394 .https://doi.org/10.3389/fmicb.2015.01394-
dcterms.bibliographicCitationBEGUM, Sharmin. QUACH, Jeanie y CHADEE, Kris. (2015). Immune Evasion Mechanisms of Entamoeba histolytica: Progression to Disease. Front Microbiol. 2015 Dec 15;6:1394. https://doi.org/10.3389/fmicb.2015.01394-
dcterms.bibliographicCitationCATTANEO, Fabio. GUERRA, Germano. PARISI, Melania. DE MARINIS, Marta. TAFURI, Domenico. CINELLI, Mariapia. AMMENDOLA, Rosario. Cell- Surface Receptors Transactivation Mediated by G Protein-Coupled Receptors. Int. J. Mol. Sci. 2014, 15, 19700-19728.-
dcterms.bibliographicCitationCONOR, Doherty.(2017) Host-Pathogen Interactions: The Role of Iron, The Journal of Nutrition, Volume 137, Issue 5, 1 May 2007, Pages 1341–1344, https://doi.org/10.1093/jn/137.5.1341-
dcterms.bibliographicCitationFONSECA COBOS, Zaida. DÍAZ GODÍNEZ, Cesar. MORA, Nancy. ALEMÁN, Omar. URIBE QUEROL, Eileen. CARRERO, Julio y ROSALES, Carlos. (2018). Entamoeba histolytica Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation. Frontiers in Cellular and Infection Microbiology, 8, 226. http://doi.org/10.3389/fcimb.2018.00226-
dcterms.bibliographicCitationGALLI, Soledad. OLAF, Jahn. HITT, Reiner. HESSE, Doerte. OPITZ, Lennart. PLESSMANN, Uwe. URLAUB, Henning. PODEROSO, Juan Jose, JARES ERIJMAN, Elizabeth y JOVIN, Thomas. (2009). A New Paradigm for MAPK: Structural Interactions of hERK1 with Mitochondria in HeLa Cells. PLoS ONE, 4(10), e7541. http://doi.org/10.1371/journal.pone.0007541-
dcterms.bibliographicCitationGALLI, Soledad. OLAF, Jahn. HITT, Reiner. HESSE, Doerte. OPITZ, Lennart. PLESSMANN, Uwe. URLAUB, Henning. PODEROSO, Juan Jose, JARES ERIJMAN, Elizabeth y JOVIN, Thomas.. (2009). A New Paradigm for MAPK: Structural Interactions of hERK1 with Mitochondria in HeLa Cells. PLoS ONE, 4(10), e7541. http://doi.org/10.1371/journal.pone.0007541-
dcterms.bibliographicCitationGONZALEZ SALAZAR, Francisco. MEESTER, Irene. GUZMÁN DE LA GARZA, Francisco. SAMPAYO REYES, Adriana. GARZA GONZÁLEZ, Jesus. MONSIVAIS DÍAS, Ofelia. BARBA DÁVILA, Bertha. HERNÁNDEZ GARCÍA, Magda y VARGAS VILLARREAL, Javier et al., ““CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica,” Journal of Parasitology Research, vol. 2018, Article ID 2796516, 6 pages, 2018. https://doi.org/10.1155/2018/2796516.-
dcterms.bibliographicCitationHALL, Belinda. SMITH, Emma. LANGER, Wolfram. JACOBS, Louisa. GOULDING, David y FIELD, Mark. (2005). Developmental Variation in Rab11-Dependent Trafficking in Trypanosoma brucei. Eukaryotic Cell, 4(5), 971–980. https://doi.org/10.1016/j.hoc.2004.07.005-
dcterms.bibliographicCitationKHUN, Heng. KIRBY, Shane y CRAING, Lee. (1998). A Neisseria meningitidis fbpABCMutant Is Incapable of Using Nonheme Iron for Growth. Infection and Immunity, 66(5), 2330–2336.-
dcterms.bibliographicCitationLEON, Nidia. ORDAZ, Cynthia. REYES LÓPEZ, Magda. y DE LA GARZA, Mireya. Microbicidal action of lactoferrin and lactoferricin and their synergistic effect with metronidazole in Entamoeba histolyticaiochemistry and Cell Biology, 2006, 84:327-336, https://doi.org/10.1139/o06-060-
dcterms.bibliographicCitationLINFORD, Alicia. EDWARDS, Thomas. SHERMAN, Nicholas. VAN, Wesley. STEWART, Lance. MYLER, Peter. STAKER, Barth. JIANG, Nona y PETRI, William. (2014). Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase. Molecular and Biochemical Parasitology, 193(1), 33–44. http://doi.org/10.1016/j.molbiopara.2014.01.003-
dcterms.bibliographicCitationMCCUBREY, James. STEELMAN, Linda. BERTRAND, Fred. DAVIS Nicole. SOKOLOSKY, Melissa. ABRAMS, Steve. MONTALTO, Giuseppe. D’ASSORO, Antonino. LIBRA, Massimo. NICOLETTI, Ferdinando. MAESTRO, Roberta. BASECKE, Jorg. RAKUS, DARIUSZ, Gizak. AGNIESZKA, Zoya. DEMIDENKO, Lucio. MARTELLI, Alberto y CERVELLO, Melchiorre. (2014). GSK-3 as potential target for therapeutic intervention in cancer. Oncotarget, 5(10), 2881–2911. MOONAH, Shannon. JIANG, Nona y PETRI, William. (2013). Host Immune Response to Intestinal Amebiasis. PLoS Pathogens, 9(8), e1003489. http://doi.org/10.1371/journal.ppat.1003489-
dcterms.bibliographicCitationPIÑA VÁZQUEZ, Carolina. REYES LÓPEZ, Magda. y DE LA GARZA, Mireya. (2012). Transferrin Binding Proteins as a Means to Obtain Iron in Parasitic Protozoa. Submitted: November 18th 2011Reviewed: May 15th 2012Published: September 19th 2012 https://www.intechopen.com/books/binding-protein/transferrin-binding-proteins-as-a-means-to-obtain-iron-in-parasitic-protozoa-
dcterms.bibliographicCitationSANDOVAL, Claudio. SOMASUNDARAM, Jayabose y ALVIN, Eden.(2004)Trends in diagnosis and management of iron deficiency during infancy and early childhood,Hematology/Oncology Clinics of North America, Volume 18, Issue 6,2004,Pages 1423-1438,ISSN 0889-8588-
dcterms.bibliographicCitationSERRANO LUNA, Jesús. PIÑA VÁZQUEZ, Carolina. REYES LÓPEZ, Magda. ORTIZ ESTRADA, Guillermo y DE LA GARZA, Mireya. (2013). Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors. Journal of Tropical Medicine, 2013, 890603. http://doi.org/10.1155/2013/890603-
dcterms.bibliographicCitationSERRANO LUNA, Jesús. PIÑA VÁZQUEZ, Carolina. REYES LÓPEZ, Magda. ORTIZ ESTRADA, Guillermo y DE LA GARZA, Mireya. (2013). Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors. Journal of Tropical Medicine, 2013, 890603. http://doi.org/10.1155/2013/890603-
dcterms.bibliographicCitationSERRANO LUNA, Jesús. y REYES LÓPEZ, Magda. (2015) “Transferrin: Endocytosis and Cell Signaling in Parasitic Protozoa,” BioMed Research International, vol. 2015, Article ID 641392, 12 pages, 2015. https://doi.org/10.1155/2015/641392.-
dc.description.versionEj. 1spa
dc.contributor.roleDe La Garza Amaya, Mireyaspa
dc.contributor.roleReyes, Magdaspa
dc.identifier.localT 17.18 S162c-
dc.rights.accessrightsinfo:eu-repo/semantics/embargoedAccessspa
dc.rights.creativecommonsAtribución-NoComercialspa
dc.subject.proposalEntamoeba histolyticaspa
dc.subject.proposalHierrospa
dc.subject.proposalTransferrinaspa
dc.subject.proposalAmebiasisspa
dc.subject.proposalFosforilaciónspa
dc.subject.proposalVías de señalizaciónspa
dc.type.dcmi-type-vocabularyTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.description.abstractenglishIron is one of the most abundant elements on Earth and the most abundant metal in the human body. This element is decisive for the development of life because almost all the organisms need iron to perform different biological activities. In humans, the concentration of Fe is regulated by several proteins that contain this element, such as transferrin (TF). TF carries Fe to every cell that needs it and keeps it away from pathogens. These organisms have developed different strategies for obtaining iron. One of them is the expression of specific transferrin receptors located in the plasma membrane. This is the case of Entamoeba histolytica, a microorganism that requires Fe to survive and in which it has been observed that TF binds to its receptor, signal transduction pathways related to its activation are important in proliferation.The study of transferrin receptors and other proteins related to signaling pathways is important because these proteins could be used as therapeutic targets due to their specificity. In this report, both the culture and the obtaining of proteins that present a state of phosphorylation by the use of TF in E.histolytica are described. The expected scope of the present work is to contribute to the study of the use of the transferin by identifying the proteins that participate in the signaling pathways and that are useful as therapeutic targets.spa
dc.thesis.nameBacteriología y Laboratorio Clínicospa
dc.thesis.disciplineFacultad de Ciencias de la Salud - Bacteriología y Laboratorio Clínicospa
dc.thesis.levelPregradospa
Appears in Collections:ACCA. Trabajos de Grado



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.