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Title: Immunological Memory to Zika Virus in a University Community in Colombia, South America
Authors: Cárdenas Sierra, Denny-Miley
Jaimes, Miguel A.
Vega, Leidy D.
Oliveros, Nicolas L.
Soto, Javier-Andrés
Chía, Claudia R.
Osorio, Jorge E.
Ciuoderis, Karl A.
Keywords: Cytokines
Flavivirus infection
Immunological memory
Seroepidemiologic studies
Zika virus
Issue Date: 29-May-2020
Abstract: Zika virus appeared in South America in 2015, generating alarm worldwide as it causes microcephaly and autoimmunity. This study aims to determine the serological footprint of the incoming epidemic in a student community and to characterize the memory functional cell response during post convalescence. In a cross-sectional study, Zika-specific IgG using LIA immunoassay was found in 328 university students (CI=95%), while in the second phase, the functional cellular memory response for IFN-γ and IL-2 was quantified using post-stimulus ELISpot with inactivated virus, starting with individuals seropositive for Zika and control individuals (seropositive only for Dengue and seronegative for Zika-Dengue). Depending on the antigen used, memory humoral response (IgG) against Zika Virus was observed in >60% of the population; seropositivity for NS1 was 21.1% higher than E antigen with high intensity. The analysis of cell functionality in 22 individuals seropositive for Zika virus revealed either IFN-γ+ or IL-2+ cells in 86.3% of cases (Th1 profile), presenting multifunctionality in 50% (11 individuals), 64% of which presented> 6 SFC/104 PBMCs (>600 SFC/106 PBMC), reflecting memory circulating cells. A good agreement (Kappa= 0.754) was observed between the coexistence of both cellular and humoral responses but not in their intensity.
Description: Digital
Appears in Collections:DCAAA. Artículos de Investigación

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