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García Sánchez, Liliana Torcoroma Cristancho, Laura Maritza Vera, Erika Patricia Begambre, Oscar 2019-07-30T19:44:25Z 2019-07-30T19:44:25Z 2015-06 1738-8872 1017-7825 https://repositorio.udes.edu.co/handle/001/3499 10.4014/jmb.1406.06079 14 p. This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm’s initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems. application/pdf eng Journal of Microbiology and Biotechnology Derechos Reservados - Universidad de Santander, 2015 https://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://www.jmb.or.kr/submission/Journal/025/JMB025-10-19_FDOC_1.pdf A new Multiplex-PCR for urinary tract pathogens detection using primer design based on an evolutionary computation method Artículo de revista http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text http://purl.org/redcol/resource_type/ART Primer Design Multiplex-PCR Urinary Tract Infections Uropathogens Hybrid Particle Swarm Optimization http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/version/c_970fb48d4fbd8a85 Publicationopen.access