Examinando por Autor "Jauregui Sierra, Karen Tatiana."

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    Identificación de tipos de Bacterias Endosimbióticas en Amebas de vida libre aisladas en muestras de suelo del Municipio de TIBÚ, NORTE DE SANTANDER 2018.
    (Cúcuta: Universidad de Santander, 2019, 2019-07-03) Jauregui Sierra, Karen Tatiana.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.
    Free-living amoebas are highly pathogenic microorganisms distributed worldwide, these microorganisms are called amphizoic, since they are capable of living in the environment and act as parasites in humans, belong to the group of Rhizopod protozoa of the order amoebida (Acanthamoeba ) and of the order Schizopyrenida (Naegleria and Balamuthia), are distributed worldwide in various reservoirs such as swimming pool waters, wells, ponds, rivers, water in the air, among others. This group of protozoa produces different pathologies such as primary amebic meningoencephalitis (MAP) by the genus Naegleria; amebic granulomatous encephalitis (GAS) by the genera Acanthamoeba, Balamuthia and Sappinia; which are of clinical relevance since they can compromise the central nervous system; In addition, Acanthamoeba and Balamuthia have been linked to eye injuries such as keratitis and corneal ulcers, as well as primary lesions of the skin. The objective of this study was to identify the interaction of freeflowing amoebas with endosymbiotic bacteria present in soil samples from Tibú Norte de Santander municipality. for this, a series of sampling (3) was carried out in five selected sites within the urban helmet of the municipality, the collected samples were transported in sterile media and were planted in ANN hangars with inactivated E. coli. The samples had a follow-up of 20 days in which a series of peals were made to obtain a pure culture. A quantity of three rings was needed to obtain the pure culture, which was followed by sowing in biochemical media such as the biochemical batteries of citrate, Lía, Tsi and SIM to observe the amoebic endosymbiosis and verify whether the amoeba harbored bacteria, whether Gram negative or Gram positive. Achieving thus isolating amoebas of the genus Acanthamoeba, Naegleria, Balamuthia, Vermamoeba in our work we observed morphology of Gram negative bacteria product of the endosymbiotic mechanism of the genus Acinetobacter and Pseudomonas. As a conclusion, it was possible to observe the presence of amoebas and highly pathogenic bacteria contained in soil samples, which constitutes an important signal in the people of this municipality and public health entities. Recommending taking the necessary measures of prevention to these highly pathogenic microorganisms.