Examinando por Autor "Ortega Cuadros, Mailen"

Mostrando 1 - 5 de 5
  • Publicación
    Acceso abierto
    Actividad antimicrobiana de aceites esenciales de Lippia alba y cymbopogon citratus sobre streptococcus mutans y citotoxicidad en células cho
    (2016) Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Mena Álvarez, O.; Martínez Pabón, M.C.; Galvis Pareja, D.; Merini, Luciano J.
    Background: Dental caries is a complex in- fectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary f low, buffering capa- city and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not com- pletely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capabi- lity of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromato- graphy with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mu- tans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium. Results: The major components in both essential oils were Geraniol and Citral. Lippia alba essential oil applied in concen- tration of 0.01 mg / 100 mL removed 95.8% of S. mutans biofilm and C. citratus e s s ent i a l o i l s h o w e d a removal activity of 95.4% in the concentrations 0.1, 0.01mg /100 mL and 93.1% in concentration 0.001 mg / 100 mL. None of the essential oils showed toxicity to CHO cells in a 24-hour treatment, with signif icant differences in relation to the control with methanol (P = 0.00) which inhibits most cells. Conclusions: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biof ilms and null cytotoxicity, evidencing a po- tential use in treating and preventing dental caries.
  • Publicación
    Acceso abierto
    Efectividad de Beauveria bassiana (Baubassil®) sobre la garrapata común del ganado bovino Rhipicephalus microplus en el Departamento de la Guajira, Colombia
    (2018-05-07) Tofiño Rivera, Adriana Patricia; Pedraza Claros, Bertilda; Ortega Cuadros, Mailen; Perdomo Ayola, Sandra C.; Moya Romero, Diana Carolina
    The effectiveness of Baubassil® (Beauveria bassiana) on the tick Rhipicephalus (Boophilus) microplus was evaluated. In the in vitro phase, the optimal germination temperature of the fungus was determined by microcultures at 25, 30 and 35 °C and the mortality of ticks. In the field, in a randomized design, 30 cebuine cows; 15 cows were treated with Baubassil® once a week at a 1 × 106 conidia/ml, and a control group was left untreated. It was observed that at temperatures of 25, 30 and 35 °C, germination of the entomopathogen was 97.5, 94.7 and 6.3%, respectively. In animals treated with Baubassil® obtained a corrected effectiveness of 96.8% on ticks in the fifth week of application. The use of Baubassil® would be a valid alternative for controlling the cattle tick under the agroecological conditions of the Dry Caribbean.
  • Publicación
    Acceso abierto
    Effect of agricultural inputs and essential oils on the soil of vegetables in Colombia's Caribbean region
    (2018) Tofiño Rivera, Adriana Patricia; Melo Ríos, Aslenis Emidia; Merini, Luciano J.; Ortega Cuadros, Mailen; Mena Rodríguez, Eduardo
    A contribuição das hortaliças para a segurança alimentícia e para o desenvolvimento econômico da Colômbia, bem como para a problemática ambiental mundial, justifica o interesse de elaborar estratégias produtivas sustentáveis para a agrocadeia. Desenvolveu-se um estudo com o objetivo de avaliar o efeito da aplicação de óleos essenciais e agroinsumos em cultivos de ají (Capsicum annuum), feijão e berinjela em Codazzi, Cesar. A metodologia incluiu a análise de compatibilidade de pesticidas utilizados nessas hortaliças e óleos de Lippia alba e Cymbopogon citratus, no que se refere ao efeito biocida in vitro em cepas nativas de Macrophomina phaseolina, Phytophthora capsici e Colletotrichum gloeosporioides. Em campo, aplicou-se tiabendazol de maneira individual e combinado com os óleos. Foram medidos indicadores físico-químicos e microbiológicos do solo, a incidência de pragas e doenças, e o desempenho dos cultivos. In vitro, os óleos controlaram até 97 % dos fitopatógenos e mostraram compatibilidade com carbendazim e tiabendazol. Em campo, foi observado um controle de doenças de até 67 % com C. citratus + tiabendazol a respeito do grupo controle (p = 0,00), desempenhos próximos à média regional, e melhores condições microbiológicas e físico-químicas do solo. Em conclusão, existem diferenças no efeito edáfico entre tratamentos, já que o agroquímico e a combinação de óleos foram mais favoráveis que o efeito individual de cada produto nas variáveis avaliadas. Isso leva a continuar realizando. avaliações com óleos em campo, para dilucidar a duração dos efeitos descritos.
  • Publicación
    Acceso abierto
    Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
    (2016-10-17) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Galvis Pareja, D.; Jiménez Rios, Hedilka; Merini, Luciano J.; Martínez Pabón, M.C.
    Background Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Chemical compounds studied in this article List of up to 10 names of chemical compounds studied in the article. Geranial. Neral. Myrcene. Geraniol. (E)-Caryophyllene. trans-Verbenol. Geranyl acetate. cis-Verbenol. Germacrene D. 37-Dimethyl-26-octadiene-1-ol
  • Publicación
    Acceso abierto
    Vigilancia tecnológica de plantas aromáticas : De la investigación a la consolidación de la agrocadena colombiana
    (2017-05) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Melo Ríos, Aslenis Emidia; Mier Giraldo, Helen Jhoana
    El sector de plantas aromáticas, medicinales y condimentarias reúne una amplia variedad de especies nativas e introducidas con potencial agroindustrial. Para contribuir al diagnóstico tecnológico del sector en Colombia y brindar elementos de planificación estratégica hacia mejores niveles de desarrollo e internacionalización, se priorizaron ciertas especies aromáticas en este artículo y se planteó una vigilancia tecnología del 2004 al 2014. Se hizo una búsqueda de artículos científicos y patentes en ocho bases de datos en el ámbito nacional, iberoamericano y mundial según temáticas priorizadas. Los datos se procesaron mediante Microsoft Excel, EndNote, Vantage Point (2014) y Thomson Data Analyzer. A nivel nacional, la temática más registrada fue el material de siembra y el mejoramiento genético, mientras que en Iberoamérica y el mundo, fue el efecto biocida. Los países líderes en investigaciones sobre las aromáticas vigiladas fueron Brasil, Colombia y Cuba. A nivel internacional, los líderes en producción científica en PubMed y Scopus fueron India, EE. UU., Brasil y Colombia. La tendencia mundial de patentes es creciente, y su principal enfoque es “Necesidades corrientes de la vida”. En conclusión, la vigilancia tecnológica ofreció una visión global de avances y tendencias en investigación y desarrollo tecnológico en áreas temáticas vinculadas con las plantas aromáticas. Además, se identificaron posibles aliados investigativos y fluctuación de la producción científica a través del tiempo.