Examinando por Materia "Amebas de Vida Libre"

Mostrando 1 - 4 de 4
  • Publicación
    Restringido
    Determinación de Amebas de vida libre y Bacterias Endosimbióticas en aguas del área Periurbana del RIO PAMPLONITA, NORTE DE SANTANDER.
    (Cúcuta: Universidad de Santander, 2017, 2017-11-30) Chacón Higuera, Wendy Lisbeth.; Alfonso Nova, Leidy Tatiana.; Perdomo Domínguez Yojanna.; Contreras Rangel, Jael.
    Las amebas de vida libre son microorganismos ubicuos, que se han relacionado con patologías mortales para el ser humano, y su potencial virulento se vincula directamente con su capacidad termotolerante y la relación de endosimbiosis que puede crear con bacterias resistentes a la lisis amebiana, lo cual puede ser fatal para la salud. Como objetivo se planteó determinar amebas de vida libre y bacterias endosimbióticas en aguas del área periurbana del rio Pamplonita. Para ello se realizaron dos muestreos en los cuales se recolectaron muestras de agua de siete puntos del rio Pamplonita, específicamente puntos del área periurbana, se empleó el método de filtración por membrana y se sembró en medios de cultivo de agar no nutritivo con sales necesarias para la viabilidad de las amebas. Al analizar los factores microbiológicos del agua se halló alta presencia de coliformes, lo cual favorece el crecimiento de amebas de vida libre en el río.
  • Publicación
    Restringido
    Determinación de amebas de vida libre y de bacterias endosimbióticas potencialmente patógenas en aguas del área RURAL DEL RIO PAMPLONITA, NORTE DE SANTANDER.
    (Cúcuta: Universidad de Sántander, 2018, 2018-05-23) Avellaneda Figueroa, Yendy Andriyirley; Ramírez Tocora, Jorge Andrés.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.
    Free-living amoebae (FLA) are ubiquitous microorganisms’ that can affect to human being cause several pathologies, through their resistance mechanisms, which is really hard “thermotolerance”. However, this microorganisms phagocyte another ones: fungi, bacteria and virus, but the last ones can do endosymbiotic relationship, and the amoebae confers resistance to their habitat and increase the pathogenic potential, that’s why it constitutes a huge problem to Public Health. The aim of this study was demonstrate the presence of free living amoebae and endosymbiotic bacterias potentially pathogenic in samples: Bocatoma El Rosal, Monteadentro, La Naciente, Estación Limnimétrica Curva Los Adioses, Pamplonita, El Diamante and Pamplonita, to characterize their distribution. Developing a field-descriptive study through of recollection of water samples and filtrated to inoculate No nutritive Agar enrichment with inactive Escherichia coli.
  • Publicación
    Restringido
    Endosimbiosis de Naegleria SPP y Cianobacterias productoras de Microcistinas aisladas de Ambientes hostiles en NORTE DE SANTANDER.
    (Cúcuta: Universidad de Santander, 2018, 2018-12-11) Villamizar Avellaneda, Génesis Nathaly.; Escalante Riveros, Jessica Johanna.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.
    Naegleria spp. Amoeba of free life characterized presents pathogenic human species, thermophilic properties and capacity to act as endosimbion of other groups of microorganisms, involves microbial relationships and makes possible the survival of the same to hostile conditions. The microorganisms that have been described as resistant to amoebic lysis, are the cyanobacteria, which are prokaryotes that, among other properties, have been characterized by carrying out photosynthesis, but there is a small group of these microorganisms that carry out a alternating metabolism, triggering the release of microcystins such as type D, described as a hepatotoxin with protumoral activity product of organisms such as Microcystis sp., Planktothrix sp. and Anabaena sp. The objective of this research is to establish the endosymbiosis of Naegleria spp. and cyanobacteria producing microcystins isolated from hostile environments in Norte de Santander. The research was based on 4 phases, in 1 place the collection of the samples in the hostile environments (Residual, Thermal and Rivers).
  • Publicación
    Restringido
    Identificación de tipos de Bacterias Endosimbióticas en Amebas de vida libre aisladas en muestras de suelo del Municipio de TIBÚ, NORTE DE SANTANDER 2018.
    (Cúcuta: Universidad de Santander, 2019, 2019-07-03) Jauregui Sierra, Karen Tatiana.; Ríos Ramírez, Yesmit-Karina.; Contreras Rangel, Jael.
    Free-living amoebas are highly pathogenic microorganisms distributed worldwide, these microorganisms are called amphizoic, since they are capable of living in the environment and act as parasites in humans, belong to the group of Rhizopod protozoa of the order amoebida (Acanthamoeba ) and of the order Schizopyrenida (Naegleria and Balamuthia), are distributed worldwide in various reservoirs such as swimming pool waters, wells, ponds, rivers, water in the air, among others. This group of protozoa produces different pathologies such as primary amebic meningoencephalitis (MAP) by the genus Naegleria; amebic granulomatous encephalitis (GAS) by the genera Acanthamoeba, Balamuthia and Sappinia; which are of clinical relevance since they can compromise the central nervous system; In addition, Acanthamoeba and Balamuthia have been linked to eye injuries such as keratitis and corneal ulcers, as well as primary lesions of the skin. The objective of this study was to identify the interaction of freeflowing amoebas with endosymbiotic bacteria present in soil samples from Tibú Norte de Santander municipality. for this, a series of sampling (3) was carried out in five selected sites within the urban helmet of the municipality, the collected samples were transported in sterile media and were planted in ANN hangars with inactivated E. coli. The samples had a follow-up of 20 days in which a series of peals were made to obtain a pure culture. A quantity of three rings was needed to obtain the pure culture, which was followed by sowing in biochemical media such as the biochemical batteries of citrate, Lía, Tsi and SIM to observe the amoebic endosymbiosis and verify whether the amoeba harbored bacteria, whether Gram negative or Gram positive. Achieving thus isolating amoebas of the genus Acanthamoeba, Naegleria, Balamuthia, Vermamoeba in our work we observed morphology of Gram negative bacteria product of the endosymbiotic mechanism of the genus Acinetobacter and Pseudomonas. As a conclusion, it was possible to observe the presence of amoebas and highly pathogenic bacteria contained in soil samples, which constitutes an important signal in the people of this municipality and public health entities. Recommending taking the necessary measures of prevention to these highly pathogenic microorganisms.