Examinando por Materia "Lactonases"

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  • Publicación
    Restringido
    Caracterización de la lactonasa variante Epp 9 derivada del gen aiiA de Bacillus thuringiensis en la inhibición de los autoinductores C4-HSL y 3-oxo-C12-HSL de Pseudomonas aeruginosa
    (Universidad de Santander, 2024-11-28) Pérez-Vergel, Astrid Carolina; Suárez-Barera, Miguel Orlando; Valdivieso-Quintero, Wilfredo; Hernández-Peñaranda, Indira Paola; Laboratorio de Biología Molecular y Biotecnología
    La resistencia a los antibióticos es uno de los problemas más importantes en que hay en la salud humana la cual ha incrementado los costos médicos, la estancia hospitalaria y la mortalidad (Jiménez, 2020). Por este motivo la inhibición del QS se ha considerado como un enfoque terapéutico atractivo, para prevenir los fenotipos resistentes y virulentos debido a que es más específica e impone una menor presión selectiva hacía el patógeno así disminuyendo la probabilidad de que la bacteria genere resistencia (Espinoza & Esparza., 2021).
  • Publicación
    Acceso abierto
    Identification, cloning and lactonase activity of recombinant protein of N-acyl homoserine lactonase (AiiA) from Bacillus thuringiensis 147-115-16 strain
    (2014-07) Florez, Alvaro M.; González, Adriana; Pedroza, Carmen Julia; Correa, Elizabeth; Rueda Forero, Nohora Juliana; Orduz, Sergio
    The quorum-quenching N-acyl homoserine lactonases are a family of bacterial metalloenzymes that participate in degradation of N-acyl homoserine lactones (AHLs), disrupting the quorum sensing system of gram negative bacterial species. From a collection of Bacillus thuringiensis strains isolated in Colombia from plants and exhibiting toxic activity against lepidopteran insects, 310 bacterial isolates were tested to determine lactonase activity by using biosensor systems in presence of synthetic N-hexanoyl-L-homoserine lactone (C6-HSL) and N-octanoyl-L-homoserine lactone (C8-HSL). From them, 251 strains showed degrading activity to both C6-HSL and C8-HSL, 57% exhibited degrading activity to C6-HSL and 43% to C8-HSL. One B. thuringiensis strain, denoted as 147-115-16, that exhibit high degrading activity to C6-HSL and C8-HSL, was able to attenuate soft rot symptoms in infected potato slices with Pectobacterium carotovorum. This strain contains an homologous of the aiiA gene that was cloned, sequenced and expressed in Esherichia coli DE3. The recombinant protein AiiA147-11516 display activity to C6-HSL, C8-HSL, N-(β-ketocaproyl) (3-O-C6-HSL) and N-3-oxo-dodecanoyl (3-O-C12-HSL). The recombinant strain in the presence of P. caratovorum cultures was able to attenuate the infection, suggesting that it interferes either on the accumulation or response to the AHLs signals. Acording to this data and based on previous report from recombinant AiiA147-11516, this enzyme exhibit activity to wide range of catalytic substrates suggesting its industrial application in the disease control programs by plants transformation.