Examinando por Materia "Lipolytic Yeast"

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  • Publicación
    Acceso abierto
    Differential protein profiles of the lipolytic yeast candida palmioleophila under different growth conditions
    (2018-05-01) Rincón, Leidy Johanna; Agualimpia Valderrama, Bayron-Enrique; Zafra, German
    This work aimed to stablish the influence of different carbon and nitrogen sources, including vegetable oils, on the variability of the protein profiles of an oil and grease-degrading strain of Candida palmioleophila. Production of biomass and protein by C. palmioleophila strain SACL11 was evaluated under eight different culture conditions, which provided palm oil or sunflower oil as sole carbon source and ammonium sulfate as sole nitrogen source, during 48 h at 30 °C. Protein profiles from C. palmioleophila crude extracts were obtained by SDS-PAGE every 12 h and analyzed with bioinformatic programs. The results showed that treatments providing the highest concentrations of each of the carbon and nitrogen sources resulted in a higher biomass production, with sunflower oil being the carbon source that produced the highest values and an overall faster growth. Proteins of approximately 63 KDa and 28 KDa were detected only in protein extracts obtained from media containing palm or sunflower oil as carbon source, suggesting a key role of these proteins in the hydrolysis of oils. Furthermore, the molecular weights of these proteins were similar to several reported lipases and esterases from Candida rugosa and other related species, reinforcing their possible function. In conclusion, this work identified and reported for the first time differential protein profiles of the lipolytic yeast C. palmioleophila in response to different growth conditions, and found evidence of the involvement of lipase-like proteins during the metabolism of vegetable oils. This give insight about the enzymes involved in grease metabolism and reinforces the potential of this promising microorganism to be used as an excellent bioremediation agent in fat, oil and grease-polluted environments.
  • Publicación
    Acceso abierto
    Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila
    (2018-05-01) Rodríguez Mateus, Zully; Vera Pacheco, Katerine; Zafra, German
    In this study we analyzed the genetic variability of lipase gene sequences from eight oil and grease-degrading strains of Candida palmioleophila and to relate it to their degrading ability. The genetic variability of lipase genes was analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and low-stringency single specific primer- PCR (LSSP-PCR), in order to obtain specific DNA fingerprints from each strain, which were subsequently compared by bioinformatic programs. DNA fingerprints were contrasted to the ability of each strain to remove palm oil in liquid culture. The results showed that at least three genes encoding lipases are present in C. palmioleophila, two of them resembling the LIP2 and LIP6 genes of C. albicans. DNA fingerprints obtained by LSSP-PCR revealed differences in the sequences of C. palmioleophila lipase genes, which allowed to group the strains according to their degrading activity. C. palmioleophila strains SACL05, SACL08 and SACL11, which showed the highest removal of palm oil after 72 h (77 to 79 % removal), were grouped in a single clade in dendrograms. Similarly, strains SACL01, SACL03, SACL06 and SACL09, which showed intermediate removal activity of palm oil (54 to 76%) grouped in a different clade. This suggests the genetic variability in lipase genes is directly related to the differences found in the efficiency of degradation of oils. On the other hand, DNA fingerprints obtained by PCR-RFLP did not allow to differentiate the strains and did not generate changes in the bands patterns between the analyzed strains. In conclusion, this study reported for the first time the detection and characterization of lipase genes from the lipolytic yeast Candida palmioleophila, and their association to the degradation of oils.