Examinando por Materia "PCR anidado"

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  • Publicación
    Acceso abierto
    Comparación de la PCR anidada y PCR convencional en el diagnóstico de Plasmodium Vivax y P. Falciparum
    (2013) Galvis Serrano, Nestor Fabián; Carrillo Hernández, Marlen Yelitza; Quintero, Fabián A.
    Malaria is diseases caused by Plasmodium sp., and in Colombia approximately 160,000 cases are annually registered, where 75% are produced by P. vivax and 24% for P. falciparum. The molecular diagnosis is performed by Nested PCR, is a general amplification followed by a specific, using two pairs of different primers. This study compared the sensitivity and specificity of nested PCR with the conventional PCR to identify P. vivax and P. falciparum in DNA from blood of patients. As samples were selected 18 patients were identified as positive by blood smear for malaria. At the DNA isolation protocols were used 3: Heidari, Chelex®100 and commercial kit from Promega. In the nested PCR and conventional primers were used to identify P. falciparum and P. vivax. The results of the amplifications obtained with conventional PCR were similar to those obtained with nested PCR, demonstrating that have the same specificity and sensitivity, which allows to conclude that the realization of the conventional PCR is sufficient for the diagnosis of P. vivax and P. falciparum, reducing time and costs in the identification. The PCR is the best method for the diagnosis of Malaria, which is more sensitive and specific compared with rapid testing and microscopic examination of drop thick, allowing the presence of the parasite in the early stages of the disease when the parasite load is minimal, and also gives the possibility of identifying cases of mixed infections.
  • Publicación
    Acceso abierto
    Marcadores serológicos y moleculares de infección por el virus de la hepatitis B en estudiantes universitarios colombianos
    (2012-10) Bautista Amorocho, Henry; Castellanos Domínguez, Yeny Zulay; Farfán García, Ana Elvira
    Introduction: Reports from the World Health Organization (WHO) show that the prevalence of hepatitis B virus (HBV) infections varies by geographical region and risk group. Purpose: The purpose of this study was to determine the prevalence of HBV infections, as well as the vaccination status, among university students from Bucaramanga. Methodology: This was a cross sectional study conducted in 2010 which included 1298 students from five universities. Serological markers for HBV infection were detected using ELISA. Viral genomes were detected with nested polymerase chain reaction (PCR). Results: Active infections were established in 0.15% of the study population, and this finding was confirmed by PCR. Resolved infections were identified in 0.60% of the population. Isolated anti-HBc antibodies were found, 30.2% of vaccinated individuals. 67.9% of the study population was susceptible. No occult HBV was detected. Conclusions: The low prevalence of HBV infections reported in this study contrasts with the intermediate epidemiological pattern described in the region. We found poor vaccination coverage and absence of occult hepatitis B among these university students.