Examinando por Materia "Producción de sustancias indólicas"

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    Evaluación a nivel in vitro del efecto de la variación nutricional sobre la actividad promotora de crecimiento vegetal en microorganismos asociados a plantas de sacha inchi (plukenetia volubilis linneo)
    (Bucaramanga : Universidad de Santander, 2019, 2019-01-22) Rojas Copete, Daniela S.; Agualimpia Valderrama, Bayron-Enrique; Acevedo Isidro, Carlos-Augusto; Orlandoni Merli, Giampaolo
    In the present work we sought to expand the criteria for the evaluation in vitro level of microorganisms with growth promoter activity (PGPM) isolated from sacha inchi organs, in order to select those that are going to be taken to the field and that have a greater beneficial effect for the crop in terms of its development. To this end, the performance response of indolic substances and solubilization of phosphates was evaluated in two strains defined in previous studies as PGPM, under different concentrations and carbon sources. Initially, the nutritional and operational requirements that allowed the stable expression of the activities in each of the microorganisms were defined, through the formulation and adjustment of the type of medium, carbon source, nitrogen, phosphorus, temperature and growth behavior. Then the response of the activities indolic substances production and solubilization of phosphates under the different carbon sources was determined.As a result, it was possible to demonstrate that for the production of indoles the highest response was obtained with tryptophan 99.97mg / L at a concentration of 5g / L while in the other carbon sources only response was obtained when the concentration was 1.25g / L in the carbon sources mannitol, xylose and glucose with values of 13.35mg / L, 10.22mg / L and 10.22mg / L of indole substances respectively, on the other hand, solubilization of phosphates was only obtained response for concentration of 10g / L with glucose as carbon source 36.30mg / L of soluble phosphate. Finally through an analysis of means; it was determined that the production of indole substances depends on the tryptophan and its concentration, while, that the solubilization of phosphates depends entirely on the glucose and its concentration in the medium for the expression of the activity.