Examinando por Materia "Virulence factors"

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  • Publicación
    Acceso abierto
    Frecuencia de los genes de virulencia iucC(aer) y hlyCA en cepas de Escherichia coli aisladas en adultos con infección del tracto urinario adquirida en comunidad de la zona metropolitana de Bucaramanga
    (Bucaramanga, Universidad de Santander, 2019, 2019-06-21) Pedrozo Rodríguez , Yanir Maruth; Martínez Vega, Ruth Aralí
    Urinary tract infections (UTI) are considered as one of the most frequent problems in primary health care and they are mostly of community origin. It is among the most common infectious diseases, with a high morbidity rate. Severity occurs in a variety of ways from asymptomatic bacteriuria, cystitis, pyelonephritis, septic shock to multiorgan failure. The main etiology of the UTIs is the bacterium Escherichia coli. One of the main sources of severity in the ITUs is the wide spectrum of virulence factors present in E. coli. Therefore, this research project sought to determine the frequency of two virulence genes of E. coli, the first is aer (aerobactin) that codes for a protein that is highly associated with iron uptake and the second gene is hly which encodes α hemolysin. The production of hemolysins is associated with extraintestinal infections, which gives E. coli a selective advantage because it favors the uptake of iron, released by lysed red blood cells, through siderophores. We analyzed 87 urine samples from patients from Bucaramanga, Santander, with UTI due to E. coli. The amplification of the DNA was determined through the PCR test at the end point, which was standardized for the detection of the two mentioned genes. It was observed that the virulence gene iucC (aer) presented a high frequency (68%) in the E. coli strains analyzed, while the hlyCA gene was infrequent (14%). The frequency of these genes in strains of uropathogenic E. coli isolated from Bucaramanga patients is within what has been reported in other countries.
  • Publicación
    Acceso abierto
    Mecanismos de virulencia de Escherichia coli enteropatógena
    (2016-07-26) Farfán García, Ana Elvira; Ariza Rojas, Sandra Catherine; Vargas Cárdenas, Fabiola Andrea; Vargas Remolina, Lizeth Viviana
    Acute diarrheal disease (ADD) is a global public health problem, especially in developing countries and is one of the causes of mortality in children under five. ADD etiologic agents include viruses, bacteria and parasites in that order. Escherichia coli bacteria it is classified as a major diarrheagenic agent and transmitted by consuming contaminated water or undercooked foods. This review compiled updates on information virulence factors and pathogenic mechanisms involved in adhesion and colonization of seven pathotypes of E. coli called enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), shigatoxigenic E. coli (STEC), enteroaggregative E. coli (EAEC) and diffusely-adherent E. coli (DAEC). A final pathotype, adherent-invasive E. coli (AIEC) associated with Crohn’s disease was also reviewed. The diarrheagenic pathotypes of E. coli affect different population groups and knowledge of the molecular mechanisms involved in the interaction with the human is important to guide research towards the development of vaccines and new tools for diagnosis and control.