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New PEPTIR-2.0 Peptide Designed for Use as Recognition Element in Electrochemical Biosensors with Improved Specificity towards E. coli O157:H7

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dc.contributor.authorRopero-Vega, Jose Luis
dc.contributor.authorRedondo-Ortega, Joshua Felipe
dc.contributor.authorRodriguez-Caicedo, Juliana Paola
dc.contributor.authorRondon-Villarreal, Paola
dc.contributor.authorFlorez-Castillo, Johanna Marcela
dc.contributor.researchgroupCibasspa
dc.date.accessioned2022-08-02T13:46:13Z
dc.date.available2022-08-02T13:46:13Z
dc.date.issued2022-04-22
dc.descriptionDigitalspa
dc.description.abstractThe detection of pathogens through alternative methodologies based on electrochemical biosensors is being studied. These devices exhibit remarkable properties, such as simplicity, specificity, and high sensitivity in monitoring pathogens. However, it is necessary to continue conducting studies that adequately improve these characteristics, especially the recognition molecule. This work aims to design and evaluate a new peptide, named PEPTIR-2.0, as a recognition molecule in electrochemical biosensors to detect E. coli O157:H7 in water. PEPTIR-2.0 was obtained from modifications of the PEPTIR-1.0 peptide sequence, which was previously reported and exhibited excellent properties for detecting and quantifying this pathogenic microorganism. PEPTIR-1.0 is a peptide analogous to the TIR (Translocated Intimin Receptor) protein capable of interacting with the Intimin outer membrane. The basis of this study was to obtain, by using bioinformatics tools, a molecule analogous to PEPTIR-1.0 that maintains its three-dimensional structure but increases the hydrophobic interactions between it and Intimin, since these intermolecular forces are the predominant ones. The designed PEPTIR-2.0 peptide was immobilized on screen-printed electrodes modified with gold nanoparticles. The detection capacity of E. coli O157:H7 in water was evaluated using electrochemical impedance spectroscopy in the presence of other microorganisms, such as P. aeruginosa, S. aureus, and non-pathogenic E. coli. The results showed that PEPTIR-2.0 confers remarkable specificity to the biosensor towards detecting E. coli, even higher than PEPTIR-1.0.eng
dc.description.researchareaCiencias Exactas y Naturalesspa
dc.format.extent10 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/ 10.3390/molecules27092704
dc.identifier.eissn1420-3049spa
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/7325
dc.language.isoengspa
dc.publisher.placeSuizaspa
dc.relation.citationendpage10spa
dc.relation.citationissue9spa
dc.relation.citationstartpage1spa
dc.relation.citationvolume27spa
dc.relation.citesCitation: Ropero-Vega, J.L.; Redondo-Ortega, J.F.; Rodríguez-Caicedo, J.P.; Rondón-Villarreal, P.; Flórez-Castillo, J.M. New PEPTIR-2.0 Peptide Designed for Use as Recognition Element in Electrochemical Biosensors with Improved Specificity towards E. coli O157:H7. Molecules 2022, 27, 2704. https://doi.org/ 10.3390/molecules27092704
dc.relation.indexedScopusspa
dc.relation.ispartofjournalMoleculesspa
dc.rightsCopyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución 4.0 Internacional (CC BY 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcehttps://www.mdpi.com/1420-3049/27/9/2704spa
dc.subject.proposalPEPTIR-1.0eng
dc.subject.proposalBioinformatics toolseng
dc.subject.proposalPathogeneng
dc.subject.proposalWatereng
dc.subject.proposalElectrochemical impedance spectroscopyeng
dc.subject.proposalHigh specificityeng
dc.titleNew PEPTIR-2.0 Peptide Designed for Use as Recognition Element in Electrochemical Biosensors with Improved Specificity towards E. coli O157:H7spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_f1cfspa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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