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Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7

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dc.contributor.authorRopero Vega, José Luis
dc.contributor.authorArdila Rosas, N.
dc.contributor.authorHernández Peñaranda, Indira Paola
dc.contributor.authorFlórez Castillo, Johanna-Marcela
dc.contributor.researchgroupCLINIUDESspa
dc.contributor.researchgroupCIBASspa
dc.date.accessioned2021-10-06T14:46:51Z
dc.date.available2021-10-06T14:46:51Z
dc.date.issued2020-03-16
dc.descriptionDigitalspa
dc.description.abstractAntimicrobial peptides arise as a very promising alternative for the treatment of infections generated by pathogenic microorganisms. The Ib-M2 antimicrobial peptide turns out to be a very promising candidate for these types of applications. However, it is required to evaluate immobilization systems that give peptides greater stability and activity in order to be used in biological systems. Given the above, this paper reports the preparation of superparamagnetic iron oxide nanoparticles (SPIONs) coated with chitosan in order to immobilize the Ib-M2 antimicrobial peptide. Structural properties of SPIONs were studied by DLS, SEM, XRD, FT-IR, XPS and the magnetic properties were evaluated by vibrating-sample magnetometer technique. The antimicrobial activity of the Ib-M2/SPIONs@Chi bioconjugate obtained was evaluated against Escherichia coli O157:H7 by determining the minimum inhibitory concentration (MIC). Results show that the SPIONs obtained have particle size between 10 and 15 nm with a magnetite-type structure which was confirmed by FT-IR and XPS. Characterization of magnetic properties evidences a superparamagnetic behavior of nanoparticles obtained. MIC results showed that Ib-M2/SPIONs@Chi bioconjugate exhibit a comparable or higher activity against E. coli in comparison with the free Ib-M2 peptide. These results show that the bioconjugate obtained can be considered for use in biomedical applications.spa
dc.description.researchareaCiencias básicas biomédicas, epidemiología, salud pública enfermedades infecciosas, enfermedades no transmisibles epidemiología, salud y desarrollo, discapacidadspa
dc.description.researchareaCiencias Básicas y Aplicadas para la Sostenibilidadspa
dc.format.extent1 p.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1016/j.apsusc.2020.146045
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/5664
dc.language.isoengspa
dc.publisherApplied Surface Sciencespa
dc.publisher.placeHolandaspa
dc.relation.citationissue15 de junio 2020spa
dc.relation.citationvolume515spa
dc.relation.citesJ.L. Ropero-Vega, N. Ardila-Rosas, Indira Paola Hernández, J.M. Flórez-Castillo, Immobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7, Applied Surface Science, Volume 515, 2020, 146045, ISSN 0169-4332, https://doi.org/10.1016/j.apsusc.2020.146045.
dc.relation.indexedApplied Surface Sciencespa
dc.relation.ispartofjournalApplied Surface Sciencespa
dc.rightsThe Editor(s) and the Author(s), 2020spa
dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://www.sciencedirect.com/science/article/abs/pii/S0169433220308011?via%3Dihubspa
dc.subject.proposalChitosaneng
dc.subject.proposalSuperparamagnetic propertieseng
dc.subject.proposalBioconjugateeng
dc.subject.proposalMinimum inhibitory concentrationeng
dc.subject.proposalGrowth kineticseng
dc.titleImmobilization of Ib-M2 peptide on core@shell nanostructures based on SPION nanoparticles and their antibacterial activity against Escherichia coli O157:H7spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_14cbspa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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