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Alteraciones histopatológicas causadas por la roya puccinia nakanishikii (Pucciniales: pucciniaceae) en plantas de cymbopogon citratus (poaceae)

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dc.contributor.authorRincón-Barón, Edgar Javier
dc.contributor.authorGutierrez-Rodriguez, Ana Mariany
dc.contributor.authorGuerra-Sierra, Beatriz Elena
dc.contributor.authorEspinosa Matías, Silvia
dc.contributor.researchgroupMicrobiotaspa
dc.date.accessioned2022-05-06T14:43:29Z
dc.date.available2022-05-06T14:43:29Z
dc.date.issued2020-02-13
dc.descriptionDigitalspa
dc.description.abstractHistopathological alterations caused by Puccinia nakanishikii rust (Pucciniales: Pucciniaceae) in Cymbopogon citratus (Poaceae) plants. Introduction: Histopathological and histochemical aspects linked to the attack of fungal rusts to plants, as well as its relation with the different spore stages are topics rather scarce in the scientific literature. Objective: To describe and analyze the histopathological and histochemical aspects of Cymbopogon citratus and its relation with the different stages of the spores from the rust fungi Puccinia nakanishikii. Methods: During the months April and August 2013, leaves healthy and infected by Puccinia nakanishikii were collected in the Northwestern scarp of the Bucaramanga-Colombia plateau. The samples with injuries on diverse developmental stages were fixated and processed according to the standard protocols for embedding and sectioning in paraffin and resin. Sections obtained from paraffin (5-7 μm) were stained with Safranin-Alcian blue and Alcian blue-Hematoxylin. On the other hand, sections obtained from resin (0.5 μm) were stained with Toluidine blue. Further, freehand sections were obtained for an autofluorescence analysis. The observations and photographic record were done via photonic microscope and epifluorescence microscope. For the observations via scanning electron microscopy (SEM), the samples were fixated in Glutaraldehyde, dehydrated with 2,2 dimethoxypropane, then desiccated to critical point and finally coated with gold. Results:The leaves are generally hypostomatic, with long and short epidermic cell forming parallel rows and showing unicellular prickle trichomes and micro-trichomes. The abaxial surface is covered by epicuticular wax forming a dense layer. The adaxial epidermis is formed by groupings of bulliform cells and epidermal cells with rectangu-lar or squared contour. In the mesophyll, there is no differentiation between palisade and spongy parenchyma, its anatomy reflects the C4 metabolism. The formation of uredosori and teliosori both hypophyllous was observed. Urediniospores are the reinfecting agents phase, they have 4-5 equatorial germ pores and echinulate wall. Teliospores have smooth wall and a persistent pedicel. The urediniospores form a germ tube, generally on the abaxial leaf surface, these tubes develop towards the stomata reaching the mesophyll interior. No appressorium were observed. The epidermis limiting the uredosorus detaches due the development and pressure that exert both the urediniospores and capitate paraphyses. As the infection progresses, autofluorescense of the chloro-phyll is lost and the cells undergo necrotic processes. Afterwards, the phloem collapses and the xylem becomes slightly disorganized. At this moment, the infection is extended along the whole leaf blade, resulting in the leaf death and the plant defoliation. On advanced stages of the infection, the uredosori showed pycnidia, probably belonging to the hyperparasite Sphaerellopsis, these structures were closely associated to the rust infected tissues. Conclusions:Puccinia nakanishikii develops on the leaves of Cymbopogon citratus producing uredosori and teliosori. Urediniospores are the reinfective stage, teliospores were only observed at late stages of the infection. The epidermis and photosynthetic tissue are severely affected by cell necrosis. The vascular tissues are deeply affected on the advances stages of the infection.eng
dc.description.researchareaCiencias Exactas y Naturales, Biotecnología Agroambiente y Saludspa
dc.format.extent22 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.15517/RBT.V68I2.37340
dc.identifier.eissn2215-2075spa
dc.identifier.issn0034-7744spa
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/6719
dc.language.isospaspa
dc.publisher.placeCosta Ricaspa
dc.relation.citationendpage382spa
dc.relation.citationissue2spa
dc.relation.citationstartpage361spa
dc.relation.citationvolume68spa
dc.relation.citesRincón Barón, E., Gutiérrez Rodríguez, A., Guerra Sierra, B., & Matías, S. (2020). Histopathological alterations caused by Puccinia nakanishikii rust (Pucciniales: Pucciniaceae) in Cymbopogon citratus (Poaceae) plants. Revista De Biología Tropical, 68(2), 361-382. https://doi.org/10.15517/rbt.v68i2.37340
dc.relation.indexedScopusspa
dc.relation.ispartofjournalRevista de Biología Tropicalspa
dc.rights© Los Autores, 2020spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://revistas.ucr.ac.cr/index.php/rbt/article/view/37340/41432spa
dc.subject.proposalHistopathology alterationseng
dc.subject.proposalRust fungieng
dc.subject.proposalTeliosporeseng
dc.subject.proposalUltrastructureeng
dc.subject.proposalUrediniosporeseng
dc.titleAlteraciones histopatológicas causadas por la roya puccinia nakanishikii (Pucciniales: pucciniaceae) en plantas de cymbopogon citratus (poaceae)spa
dc.title.alternativeHistopathological alterations caused by Puccinia nakanishikii rust (Pucciniales: Pucciniaceae) in Cymbopogon citratus (Poaceae) plantsspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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