Publicación:
Sensitivity and efficiency of RNA sample pooling for real-time quantitative polymerase chain reaction testing for SARS-CoV-2

dc.contributor.authorBautista, Leonelo E.
dc.contributor.authorVillar, Luis A.
dc.contributor.authorCleves, Mario A.
dc.contributor.authorGelvez, Margarita
dc.contributor.authorLozano-Parra, Anyela
dc.contributor.authorBueno-Ariza, Nathalia
dc.contributor.authorOrostegui-Arenas, Myriam
dc.contributor.authorMartinez-Vega, Ruth Arali
dc.contributor.authorDíaz-Galvis, Martha
dc.contributor.researchgroupSalud Comuniudesspa
dc.date.accessioned2022-08-17T22:34:15Z
dc.date.available2022-08-17T22:34:15Z
dc.date.issued2022-06-05
dc.descriptionDigitalspa
dc.description.abstractBackground: In spite of the worth of pool testing in public health, data on the sensitivity and efficiency of real-time quantitative polymerase chain reaction (RT-qPCR) pool testing for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in middle and low-income countries are limited. Methods: We mixed single specimens of extracted RNA positive for the SARS-CoV-2 envelope (E) gene by RT-qPCR with negative specimens, in pools of 4 (n=89), 8 (n=92), 16 (n=102), and 32 (n=105) specimens each. We estimated the average change in cycle threshold (Ct) for each pool size and added it to the Ct values of the first 1,350 tests in our lab, to obtain dilution-corrected Ct values. We estimated pool sensitivity as the proportion of samples with dilution-corrected Ct >40, and used it in simulations of the efficiency (tests used/true case detected) of binary split pool testing. Results: We tested 388 pools. Average Ct changes were 2.21, 2.51, 3.27, and 3.94 cycles, for pools of 4, 8, 16, and 32 specimens, respectively. Corresponding pool tests sensitivities were 91.1%, 89.6%, 85.8% and 82.5%. Pool testing was substantially more efficient than individual testing. For prevalence of 0.5% to 2.0%, the efficiency of pools of ≥8 specimens was 30% to 280% higher, and the number of people tested was 4.4 to 13.9 times higher than those of individual testing. Conclusions: Binary split pool testing substantially increases the number of people tested and the number of true cases detected per test used. This strategy is key to curtail the transmission of SARS-CoV-2, by increasing efficiency in the identification and isolation of symptomatic and asymptomatic infected individuals.eng
dc.description.researchareaCiencias Médicas y de la Saludspa
dc.format.extent9 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.21037/jphe-21-97
dc.identifier.eissn2520-0054spa
dc.identifier.issn2520-0054spa
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/7430
dc.language.isoengspa
dc.publisher.placeChinaspa
dc.relation.citesBautista LE, Villar LA, Cleves MA, Gelvez M, Lozano-Parra A, Bueno-Ariza N, Oróstegui M, Martínez-Vega RA, Díaz-Galvis M. Sensitivity and efficiency of RNA sample pooling for real-time quantitative polymerase chain reaction testing for SARS-CoV-2. J Public Health Emerg 2022;6:12.
dc.relation.indexedScopusspa
dc.relation.ispartofjournalJournal of Public Health and Emergencyspa
dc.rightsCopyright © 2009 - 2022 AME Publishing Company. All rights reserved.spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.sourcehttps://jphe.amegroups.com/article/view/8090/htmlspa
dc.subject.proposalPolymerase chain reaction (PCR)eng
dc.subject.proposalPool testingeng
dc.subject.proposalCOVID-19eng
dc.subject.proposalMass screeningeng
dc.subject.proposalSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)eng
dc.subject.proposalColombiaeng
dc.subject.proposalTesting efficiencyeng
dc.titleSensitivity and efficiency of RNA sample pooling for real-time quantitative polymerase chain reaction testing for SARS-CoV-2spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
Archivos
Paquete original
Mostrando 1 - 1 de 1
Imagen en miniatura
Nombre:
Sensitivity and efficiency of RNA sample pooling for real-time quantitative polymerase chain reaction testing for SARS-CoV-2.pdf
Tamaño:
215.88 KB
Formato:
Adobe Portable Document Format
Descripción:
Paquete de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
59 B
Formato:
Item-specific license agreed upon to submission
Descripción: