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An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells

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dc.contributor.authorJureczek, Justyna
dc.contributor.authorBergmann, Ralf
dc.contributor.authorBerndt, Nicole
dc.contributor.authorKoristka, Stefanie
dc.contributor.authorKegler, Alexandra
dc.contributor.authorPuentes-Cala, Edinson
dc.contributor.authorSoto, Javier-Andrés
dc.contributor.authorArndt, Claudia
dc.contributor.authorBachmann, Michael
dc.contributor.authorFeldmann, Anja
dc.date.accessioned2021-09-14T20:32:24Z
dc.date.available2021-09-14T20:32:24Z
dc.date.issued2019-07-29
dc.descriptionDigitalspa
dc.description.abstractRecently, we established the controllable modular UniCAR platform technology to advance the efficacy and safety of CAR T cell therapy. The UniCAR system is composed of (i) target modules (TMs) and (ii) UniCAR armed T cells. TMs are bispecific molecules that are able to bind to the tumor cell surface and simultaneously to UniCAR T cells. For interaction with UniCAR T cells, TMs contain a peptide epitope sequence which is recognised by UniCAR T cells. So far, a series of TMs against a variety of tumor targets including against the prostate stem cell antigen (PSCA) were constructed and functionally characterised. In order to facilitate their purification all these TMs are expressed as recombinant proteins equipped with an oligo-His-tag. The aim of the here presented manuscript was to learn whether or not the oligo-His-tag of the TM influences the UniCAR system. For this purpose, we constructed TMs against PSCA equipped with or lacking an oligo-His-tag. Both TMs were compared side by side including for functionality and biodistribution. According to our data, an oligo-His-tag of a UniCAR TM has only little if any effect on its binding affinity, in vitro and in vivo killing capability and in vivo biodistribution.eng
dc.description.edition1 ed.spa
dc.format.extent1 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41598-019-47044-4
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/5614
dc.language.isoengspa
dc.publisher.placeInglaterraspa
dc.relation.citationendpage15spa
dc.relation.citationissue(2019) 9:10547spa
dc.relation.citationstartpage1spa
dc.relation.citationvolumePublished On line: 29 July 2019spa
dc.relation.citesJureczek, J., Bergmann, R., Berndt, N. et al. An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells. Sci Rep 9, 10547 (2019). https://doi.org/10.1038/s41598-019-47044-4
dc.relation.ispartofjournalScientific Reportsspa
dc.rightsThe Author(s) 2019spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourcehttps://www.nature.com/articles/s41598-019-47044-4spa
dc.titleAn oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cellsspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTCASOspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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