dc.contributor.advisor	García-Sánchez, Liliana Torcoroma
dc.contributor.author	Paez-Lizcano, Silvia Catalina
dc.contributor.author	Uceda-Castellanos, Leidy Marcela
dc.contributor.jury	Hernández, Indira Paola
dc.contributor.jury	Rueda-Forero, Nohora Juliana
dc.contributor.researchgroup	Biotecnología Agroambiental y Salud - MICROBIOTA
dc.contributor.researchgroup	Manejo Clínico - CLINIUDES
dc.date.accessioned	2023-09-21T23:03:03Z
dc.date.available	2023-09-21T23:03:03Z
dc.date.issued	2023-07-19
dc.description	Digital	spa
dc.description.abstract	Antecedentes: El cáncer es considerado un desafío global de salud pública y una de las principales causas de muerte, a nivel global. Los tratamientos convencionales se basan en el uso de quimioterápicos como Doxorrubicina (Dox), que ocasionan gran variedad de efectos adversos, principalmente de tipo cardiotóxico. Así, es importante el estudio de terapias complementarias con capacidad cardioprotectora. Al respecto, polifenoles derivados de Theobroma cacao L han sido descritos como potentes antioxidantes, antiinflamatorios y cardioprotectores. Métodos: Extractos polifenólicos de semillas de clones de T. cacao L [CCN51 (A16-160) e ICS-95 (ICS-95)] fueron deshidratados mediante spray dryer y liofilización respectivamente; y encapsulados en goma arábiga al 16%. Ensayos de citotoxicidad de los encapsulados y de interacción farmacológica, en combinación con Dox, fueron realizados sobre células de miocardioblastos murinos H9c2. Técnicas de microscopía óptica y de fluorescencia fueron usadas para estimar los efectos sobre la integridad celular, el potencial de membrana mitocondrial y el estrés oxidativo. Resultados: Las terapias individuales con encapsulados de T. cacao L demostraron muy baja toxicidad sobre células H9c2 (CC50 A16-160=4.709 ± 2,0 µg/mL; CC50 ICS-95=1.916 ± 4,0 µg/mL), en comparación con Dox (CC50 10,3 ± 0,6 µg/mL). No obstante, su uso combinado con Dox presentó comportamiento sinergístico sobre H9c2, incrementando la toxicidad del quimioterapéutico [Dox+A16-160 (∑CIF= 0,4 ± 0,14) y Dox+ICS-95 (∑CIF= 0,5 ± 0,12)], a pesar de disminuir hasta cuatro veces su CC50. Igualmente, la mezcla de encapsulados de cacao con Dox incrementó la perturbación del potencial de membrana mitocondrial y el aumento de estrés oxidativo, sobre las células cardiacas. Conclusión: Los extractos polifenólicos de T. cacao L demostraron ser seguros sobre las células estudiadas, sin embargo, estudios posteriores de interacción farmacológica con Dox deben realizarse usando menores concentraciones, considerando su potencial antioxidante y cardioprotector.	spa
dc.description.abstract	Background: Cancer is considered a global public health challenge and one of the leading causes of death, worldwide. Conventional treatments are based on the use of chemotherapy drugs such as Doxorubicin (Dox), which cause a wide variety of adverse effects, mainly cardiotoxic. Thus, it is important to study complementary therapies with cardioprotective capacity. In this regard, polyphenols derived from Theobroma cacao L have been described as potent antioxidants, anti-inflammatory and cardioprotective. Methods: Polyphenolic extracts from seeds of T. cacao L clones [CCN51 (A16-160) and ICS-95 (ICS-95)] were dehydrated by spray dryer and lyophilization, respectively; and encapsulated in 16% gum arabic. Cytotoxicity of the studied encapsulates as well as drug interaction assays, in combination with Dox, were performed on H9c2 murine myocardioblast cells. Light and fluorescence microscopy techniques were used to estimate effects on cell integrity, mitochondrial membrane potential and oxidative stress. Results: Individual therapies with T. cacao L encapsulates showed very low citotoxicity on H9c2 cells (CC50 A16-160=4,709 ± 2.0 µg/mL; CC50 ICS-95=1,916 ± 4.0 µg/mL), compared to Dox (CC50 10.3 ± 0.6 µg/mL). However, its combined use with Dox presented synergistic behavior on H9c2, increasing the toxicity of the chemotherapeutic [Dox+A16-160 (∑CIF= 0.4 ± 0.14) and Dox+ISC-95 (∑CIF= 0.5 ± 0.12)], despite decreasing up to four times its CC50 value. Likewise, the mixture of cocoa encapsulates with Dox increased the perturbation of mitochondrial membrane potential and increased oxidative stress, on cardiac cells. Conclusion: The polyphenolic extracts of T. cacao L proved to be safe on the cells studied; however, further studies of pharmacological interaction with Dox should be carried out using lower concentrations, considering its antioxidant and cardioprotective potential.	eng
dc.description.degreelevel	Pregrado
dc.description.degreename	Microbiólogo Industrial
dc.description.researcharea	Tecnologías y Biotecnologías Aplicadas en el Agroambiente, Salud e Industria
dc.format.extent	32 p
dc.format.mimetype	application/pdf
dc.format.mimetype	image/png
dc.identifier.instname	Universidad de Santander
dc.identifier.local	T 33.23 P129p
dc.identifier.reponame	Repositorio Digital Universidad de Santander
dc.identifier.repourl	https://repositorio.udes.edu.co
dc.identifier.uri	https://repositorio.udes.edu.co/handle/001/9264
dc.language.iso	spa
dc.publisher	Universidad de Santander
dc.publisher.branch	Bucaramanga
dc.publisher.faculty	Facultad de Ciencias Naturales
dc.publisher.place	Bucaramanga, Colombia
dc.publisher.program	Microbiología Industrial
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dc.rights	Derechos Reservados - Universidad de Santander, 2023. Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.	spa
dc.rights.accessrights	info:eu-repo/semantics/openAccess
dc.rights.coar	http://purl.org/coar/access_right/c_abf2
dc.rights.license	Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.decs	Enfermedades Infecciosas
dc.subject.proposal	Theobroma cacao L	spa
dc.subject.proposal	Doxorrubicina	spa
dc.subject.proposal	Cardioprotección	spa
dc.subject.proposal	Cardiomioblastos	spa
dc.subject.proposal	Encapsulados polifenólicos	spa
dc.subject.proposal	Theobroma cacao L	eng
dc.subject.proposal	Doxorrubicin	eng
dc.subject.proposal	Cardioprotection	eng
dc.subject.proposal	Cardiomyoblasts	eng
dc.subject.proposal	Polyphenolic encapsulates	eng
dc.title	Potencial Cardioprotector de Encapsulados Polifenólicos de Theobroma cacao L. Sobre un Modelo In Vitro de Cardiotoxicidad Inducido por Doxorrubicina	spa
dc.title.translated	Cardioprotective Potential of Polyphenolic Encapsulates of Theobroma cacao L. on an In Vitro Model of Cardiotoxicity Induced by Doxorubicin
dc.type	Trabajo de grado - Pregrado
dc.type.coar	http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion	http://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.content	Text
dc.type.driver	info:eu-repo/semantics/bachelorThesis
dc.type.redcol	http://purl.org/redcol/resource_type/TP
dc.type.version	info:eu-repo/semantics/submittedVersion
dcterms.audience	Todas las Audiencias	spa
dspace.entity.type	Publication

Publicación: Potencial Cardioprotector de Encapsulados Polifenólicos de Theobroma cacao L. Sobre un Modelo In Vitro de Cardiotoxicidad Inducido por Doxorrubicina

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Potencial Cardioprotector de Encapsulados Polifenólicos de Theobroma cacao L. Sobre un Modelo In Vitro de Cardiotoxicidad Inducido por Doxorrubicina