Publicación:
Functional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia

Página de artículo simple
dc.contributor.authorSanchez-Martinez, Alexandra
dc.contributor.authorAcevedo-Saenz, Liliana
dc.contributor.authorAlzate-Angel, Juan Carlos
dc.contributor.authorAlvarez, Cristian M.
dc.contributor.authorGuzman, Fanny
dc.contributor.authorRoman, Tanya
dc.contributor.authorUrcuqui-Inchima, Silvio
dc.contributor.authorCardona-Maya, Walter D.
dc.contributor.authorVelilla, Paula Andrea
dc.contributor.researchgroupMasiraspa
dc.date.accessioned2022-08-09T22:01:40Z
dc.date.available2022-08-09T22:01:40Z
dc.date.issued2022-03-22
dc.descriptionDigitalspa
dc.description.abstractCD8+ T-cells play a crucial role in the control of HIV replication. HIV-specific CD8+ T-cell responses rapidly expand since the acute phase of the infection, and it has been observed that HIV controllers harbor CD8+ T-cells with potent anti-HIV capacity. The development of CD8+ T-cell-based vaccine against HIV-1 has focused on searching for immunodominant epitopes. However, the strong immune pressure of CD8+ T-cells causes the selection of viral variants with mutations in immunodominant epitopes. Since HIV-1 mutations are selected under the context of a specific HLA-I, the circulation of viral variants with these mutations is highly predictable based on the most prevalent HLA-I within a population. We previously demonstrated the adaptation of circulating strains of HIV-1 to the HLA-A*02 molecule by identifying mutations under positive selection located in GC9 and SL9 epitopes derived from the Gag protein. Also, we used an in silico prediction approach and evaluated whether the mutations found had a higher or lower affinity to the HLA-A*02. Although this strategy allowed predicting the interaction between mutated peptides and HLA-I, the functional response of CD8+ T-cells that these peptides induce is unknown. In the present work, peripheral blood mononuclear cells from 12 HIV-1+ HLA-A*02:01+ individuals were stimulated with the mutated and wild-type peptides derived from the GC9 and SL9 epitopes. The functional profile of CD8+ T-cells was evaluated using flow cytometry, and the frequency of subpopulations was determined according to their number of functions and the polyfunctionality index. The results suggest that the quality of the response (polyfunctionality) could be associated with the binding affinity of the peptide to the HLA molecule, and the functional profile of specific CD8+ T-cells to mutated epitopes in individuals under cART is maintained.eng
dc.description.researchareaCiencias Médicas y de la Saludspa
dc.format.extent14 pspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.3389/fimmu.2022.7939
dc.identifier.eissn1664-3224spa
dc.identifier.issn1664-3224spa
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/7370
dc.language.isoengspa
dc.publisher.placeSuizaspa
dc.relation.citationvolume13spa
dc.relation.citesSa´ nchez-Mart´ınez A, Acevedo-Sa´ enz L, Alzate-A´ ngel JC, A´ lvarez CM, Guzma´n F, Roman T, Urcuqui-Inchima S, Cardona-Maya WD and Velilla PA (2022) Functional Profile of CD8+ T-Cells in Response to HLAA02: 01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medell´ın, Colombia. Front. Immunol. 13:793982. doi: 10.3389/fimmu.2022.793982
dc.relation.indexedScopusspa
dc.relation.ispartofjournalFrontiers in Immunologyspa
dc.rights© 2022 The Authors, Frontiers Media S.A. All rights reservedspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución 4.0 Internacional (CC BY 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcehttps://www.frontiersin.org/articles/10.3389/fimmu.2022.793982/fullspa
dc.subject.proposalCD8+ T-cellseng
dc.subject.proposalT-cell epitopeseng
dc.subject.proposalHIV-1eng
dc.subject.proposalHLA-A*02eng
dc.subject.proposalPolyfunctionalityeng
dc.subject.proposalClass I HLA-peptide binding affinityeng
dc.subject.proposalGag-derived peptideseng
dc.titleFunctional Profile of CD8+ T-Cells in Response to HLA-A*02:01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombiaspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceTodas las Audienciasspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
Archivos
Paquete original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Functional Profile of CD8+ T-Cells in Response to HLA-A 02.01-Restricted Mutated Epitopes Derived from the Gag Protein of Circulating HIV-1 Strains from Medellín, Colombia.pdf
Tamaño:
224.49 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Paquete de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
59 B
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
AGBBA. Artículos de Investigación