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Effect of the genetic group of cows of the Gyr and Holstein breeds on the in vitro production technique of bovine embryos
(2020-08-31) Narvaez, Hector Javier; Gil-Perdomo, José Ángel
The aim of this study was to evaluate the genetic group effect of oocyte donors of the Gyr and Holstein breeds on the performance of the in vitro embryo production technique. Twelve multiparous cows were used, non-lactating, six of the Gyr breed (Bos indicus), and six of the Holstein (Bos taurus) breed. Prior to the implementation of the follicular aspiration technique, the animals were subjected to estrus synchronization through the administration of 3 mg estradiol benzoate (RIC-BE , Tecnopec Ltda., Brazil) plus the insertion of an ear implant with norgestomet (Crestar , Intervet, Brazil). Aspiration was performed seven days after the the start of the synchronization protocol. The results showed that the cows of the Gyr breed had a higher number of visualized follicles and recovered oocytes, as well as a higher cleavage and blastocyst rate on day 7. These results allow considering that, the application of the in vitro embryo production technique in the Gyr breed offers a better performance in specic parameters in comparison to the Holstein breed.