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Caracterización de la Microbiocenosis Asociada al Deterioro en Edificaciones Antiguas y Posibles Bienes de Interés Cultural en el área Metropolitana de Bucaramanga: una Aproximación Metagenómica

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dc.contributor.advisorRueda-Forero, Nohora Juliana
dc.contributor.advisorHerrera-Pineda, Diego Fernando
dc.contributor.authorGarcés-Manosalva, Rubén Darío
dc.contributor.juryValdivieso-Quintero, Wilfredo
dc.contributor.juryMantilla-Paredes, Andrea Juliana
dc.date.accessioned2023-09-26T15:32:26Z
dc.date.available2023-09-26T15:32:26Z
dc.date.issued2023-07-07
dc.descriptionDigitalspa
dc.description.abstractEl presente Trabajo de Grado tuvo como objetivo principal caracterizar la microbiocenosis asociada al biodeterioro en edificaciones antiguas y posibles bienes de interés cultural en el área metropolitana de Bucaramanga. Para llevar a cabo este análisis microbiológico, se utilizó el Laboratorio de Biología Molecular y Biotecnología de la Universidad de Santander UDES. El muestreo se realizó en la Casa de la Cultura Piedra del Sol, ubicada en Floridablanca, Santander. Se empleó la plataforma MG-RAST para lograr una identificación precisa de los microorganismos hasta nivel de género. Esta identificación se basó en el uso de metadatos detallados que proporcionaban información esencial sobre la muestra analizada, los objetivos del estudio, el lugar exacto de recolección, el tipo de librería utilizada y los genes y regiones secuenciadas. Los análisis se llevaron a cabo mediante el uso de algoritmos basados en similaridad, lo que permitió realizar asignaciones taxonómicas precisas. La concentración de ADN obtenida en el análisis fue de 500 ng/µL. En términos generales, se puede concluir que el biodeterioro que afecta los materiales que forman parte del ecosistema de un bien cultural se define como el proceso en el cual las actividades vitales de los organismos resultan en cambios indeseables en las propiedades de dicho material. Gran parte de la microbiocenosis descrita en este estudio son conocidos por su capacidad de deteriorar diversos materiales entre ellos el concreto. Es importante destacar que en esta investigación se obtuvieron datos veraces y precisos, lo que indica que el proceso de secuenciación se llevó a cabo de manera satisfactoria.spa
dc.description.abstractThe main objective of this graduate work was to characterize the microbiocenosis associated with biodeterioration in old buildings and possible assets of cultural interest in the metropolitan area of Bucaramanga. To carry out this microbiological analysis, the Laboratory of Molecular Biology and Biotechnology of the Universidad de Santander UDES. Sampling was performed at the Casa de la Cultura Piedra del Sol, located in Floridablanca, Santander. The MG-RAST platform was used to achieve an accurate identification of microorganisms down to the genus level. This identification was based on the use of detailed metadata that provided essential information about the sample analyzed, the objectives of the study, the exact place of collection, the type of library used, and the genes and regions sequenced. Analyses were carried out using similarity-based algorithms, which allowed precise taxonomic assignments to be made. The DNA concentration obtained in the analysis was 500 ng/µL. In general terms, it can be concluded that biodeterioration affecting materials that are part of the ecosystem of a cultural property is defined as the process in which the vital activities of organisms result in undesirable changes in the properties of such material. Most of the microbiocenoses described in this study are known for their ability to deteriorate various materials, including concrete. It is important to emphasize that in this research accurate and precise data were obtained, which indicates that the sequencing process was carried out satisfactorily.eng
dc.description.degreelevelPregrado
dc.description.degreenameMicrobiólogo Industrial
dc.description.tableofcontentsResumen 15 Abstract 17 Introducción 19 Marco Teórico 22 Materiales y Propiedades del Concreto en la Construcción 23 El Concreto 23 Aspectos Físicos del Concreto 23 Aspectos Químicos del Concreto 24 Factores Asociados al Deterioro y Biodeterioro del Concreto 25 Factores Abióticos 25 Factores Bióticos 26 Microbiocenosis del Concreto 26 Secuenciación de Nueva Generación 32 Secuenciación de próxima generación en el Biodeterioro 33 Illumina 33 MiSeq o HiSeq 37 Primers Empleados en la Secuenciación 39 Coeficiente de Calidad 39 Análisis Bioinformático de Secuencias 40 MG-RAST Herramienta Bioinformática 41 Estado del Arte 44 Marco Legal 50 Planteamiento del Problema 52 Justificación 56 Pregunta de investigación 58 Hipótesis 59 Objetivos 60 Objetivo General 60 Objetivo Específicos 60 Metodología 61 Lugar de Muestreo 61 Extracción y Purificación de ADN Metagenómico 62 Control de Calidad del ADN 62 Librería – Metagenómica 63 Secuenciación por Plataforma Illumina 64 Análisis de la Librería Illumina 64 Resultados 65 Extracción y Cuantificación de ADN Metagenómico 65 Análisis Bioinformático de Lecturas 65 Porcentaje de Calidad 65 Gen 16S 66 Clasificación por Dominios 66 Clasificación por Filo 66 Clasificación por Género 67 Mapa de calor 69 Diversidad Alfa α 72 Gen 18S 72 Clasificación por Dominios 72 Clasificación por Filo 72 Clasificación por Género 74 Mapa de Calor 75 Diversidad Alfa α 76 Gen ITS 76 Clasificación por Dominios 76 Clasificación por Filo 77 Mapa de Calor 79 Diversidad Alfa α 80 Discusión 81 Extracción de ADN 82 Análisis Bioinformático de Secuencias 83 Gen 16S 84 Clasificación por Dominio 84 Clasificación por Filo 86 Clasificación por Género 89 Diversidad Alfa 91 Gen 18S 92 Clasificación por Dominio 92 Clasificación por Filo 93 Clasificación por Género 96 Diversidad Alfa 98 Gen ITS 98 Clasificación por Dominios 98 Clasificación por Filo 99 Clasificación por Género 100 Diversidad Alfa 102 Conclusiones 103 Recomendaciones 105 Referencias Bibliográficas 106spa
dc.format.extent127 p
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad de Santander
dc.identifier.localT 33.23 G171c
dc.identifier.reponameRepositorio Digital Universidad de Santander
dc.identifier.repourlhttps://repositorio.udes.edu.co
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/9292
dc.language.isospa
dc.publisherUniversidad de Santander
dc.publisher.branchBucaramanga
dc.publisher.facultyFacultad de Ciencias Naturales
dc.publisher.placeBucaramanga, Colombia
dc.publisher.programMicrobiología Industrial
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dc.rightsDerechos Reservados - Universidad de Santander, 2023. Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.armarcBiodeterioro
dc.subject.armarcPatrimonio cultural
dc.subject.armarcMicroorganismos biodeteriógenos
dc.subject.armarcComunidad microbiana
dc.subject.armarcMetagenómica
dc.subject.armarcCasa de la Cultura Piedra del Sol
dc.subject.proposalBiodeterioro del concretospa
dc.subject.proposalMicrobiocenosis del concretospa
dc.subject.proposalEdificaciones antiguasspa
dc.subject.proposalPosibles bienes de interes culturalspa
dc.subject.proposalBiodeterioration of concreteeng
dc.subject.proposalMicrobiocenosis of concreteeng
dc.subject.proposalAncient buildingseng
dc.subject.proposalPossible properties of cultural interesteng
dc.titleCaracterización de la Microbiocenosis Asociada al Deterioro en Edificaciones Antiguas y Posibles Bienes de Interés Cultural en el área Metropolitana de Bucaramanga: una Aproximación Metagenómicaspa
dc.title.translatedCharacterization of the Microbiocenosis Associated With Deterioration in Ancient Buildings and Possible Assets of Cultural Interest in the Metropolitan area of Bucaramanga: a Metagenomic Approach.
dc.typeTrabajo de grado - Pregrado
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.type.coarversionhttp://purl.org/coar/version/c_71e4c1898caa6e32
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dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TP
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