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Producción de Sideróforos en Bacterias Aisladas de la Rizosfera del Cultivo de Persea americana en Chinácota, Norte de Santander

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dc.contributor.advisorGalvis-Serrano, Néstor Fabián
dc.contributor.authorGirón-Navarro Keyla Adaia
dc.contributor.authorMuñoz-Sánchez, Isabel Cristina
dc.contributor.authorRivera-Avellaneda, Liliana Valentina
dc.contributor.juryParada-Jurado, Holver Smith
dc.contributor.juryPeñaranda-Lizarazo, Elena María
dc.contributor.researchgroupDOLLY
dc.date.accessioned2025-04-30T15:52:32Z
dc.date.available2025-04-30T15:52:32Z
dc.date.issued2024-12-05
dc.descriptionDigitalspa
dc.description.abstractEl objetivo de esta investigación es establecer la producción de sideróforos en bacterias aisladas de la rizosfera del cultivo de Persea americana. La población del estudio consistió en rizobacterias presentes en el suelo rizosférico de un cultivo de P. americana ubicado en Chinácota, Norte de Santander. La muestra estuvo conformada por rizobacterias productoras de sideróforos aisladas de ocho muestras de suelo recolectadas mediante un muestreo no probabilístico por cuotas. Metodología: Las bacterias se aislaron en medios selectivos NFB y Ashby, y su capacidad para producir sideróforos se evaluó cualitativamente mediante medio CAS-agar. Además, se identificaron genes relacionados con la síntesis de sideróforos mediante PCR, diseñando cebadores específicos para compuestos como enterobactina y pseudobactina. Las especies bacterianas se caracterizaron molecular y bioquímicamente. Resultados: De las 505 colonias aisladas, 54 presentaron halos naranjas en CAS-agar, indicando actividad siderofórica. De estas, solo 24 mostraron la presencia de genes específicos en pruebas de PCR, confirmando la producción de sideróforos como pyoverdina y rhizobactina. Las bacterias identificadas incluyen Pseudomonas fluorescens, Pseudomonas aeruginosa y Enterobacter cloacae. Conclusiones: Se identificaron genes relacionados con la síntesis de enterobactina, pyoverdina, rhizobactina y pseudobactina, destacando su potencial como PGPRs. Los resultados sugieren la necesidad de investigaciones adicionales en genómica estructural y funcional para aprovechar estos microorganismos en biofertilización, biocontrol y biorremediación.spa
dc.description.abstractThe objective of this research is to establish the production of siderophores in bacteria isolated from the rhizosphere of Persea americana crops. The study population consisted of rhizobacteria present in the rhizospheric soil of a P. americana crop located in Chinácota, Norte de Santander. The sample was composed of siderophore-producing rhizobacteria isolated from eight soil samples collected using a non-probabilistic quota sampling method. Methodology: The bacteria were isolated in selective media NFB and Ashby, and their ability to produce siderophores was qualitatively evaluated using CAS-agar medium. Additionally, genes related to siderophore synthesis were identified using PCR with specifically designed primers for compounds such as enterobactin and pseudobactin. The bacterial species were characterized molecularly and biochemically. Results: Of the 505 isolated colonies, 54 exhibited orange halos in CAS-agar, indicating siderophore activity. Of these, only 24 showed the presence of specific genes in PCR tests, confirming the production of siderophores such as pyoverdine and rhizobactin. The identified bacteria included Pseudomonas fluorescens, Pseudomonas aeruginosa, and Enterobacter cloacae. Conclusions: Genes related to the synthesis of enterobactin, pyoverdine, rhizobactin, and pseudobactin were identified, highlighting their potential as PGPRs. The findings suggest the need for further research in structural and functional genomics to utilize these microorganisms in biofertilization, biocontrol, and bioremediation.eng
dc.description.degreelevelPregrado
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínico
dc.description.researchareaBiotecnologia
dc.description.tableofcontentsResumen ........................................................................................................................................ 18 Summary ....................................................................................................................................... 24 Introducción .................................................................................................................................. 26 Descripción del Problema ............................................................................................................. 27 Formulación del Problema ............................................................................................................ 28 Antecedentes de la Investigación .................................................................................................. 28 Antecedentes Internacionales ........................................................................................................ 29 Antecedentes Nacionales .............................................................................................................. 31 Antecedentes Regionales .............................................................................................................. 32 Marco Teórico ............................................................................................................................... 32 Rizosfera ....................................................................................................................................... 32 Rizobacterias ................................................................................................................................. 32 Interacción Planta Microorganismo .............................................................................................. 34 Mecanismos de Acción de las Rizobacterias ................................................................................ 35 Mecanismos de Acción Directos .................................................................................................. 35 Sideróforos .................................................................................................................................... 38 Persea americana ........................................................................................................................... 41 Justificación .................................................................................................................................. 42 Objetivos ....................................................................................................................................... 43 Objetivo General ........................................................................................................................... 43 Objetivos Específicos.................................................................................................................... 43 Metodología .................................................................................................................................. 43 Tipo de Investigación .................................................................................................................... 43 Sitio y Periodo del Estudio ........................................................................................................... 43 Población....................................................................................................................................... 44 Muestra ......................................................................................................................................... 44 Métodos......................................................................................................................................... 44 Identificación de Bacterias a Partir de Muestras de Rizosfera de Plantas de P. Americana por Medio de Técnicas Microbiológicas, Bioquímicas y Moleculares ........... 44 Determinación de la Producción de Sideróforos en Rizobacterias a Través de Ensayos Cualitativos ................................................................................................................................... 48 Detección de los Genes Relacionados con la Producción de Sideróforos .................................... 50 Técnicas e Instrumentos de Recolección de Datos ....................................................................... 52 Técnica de Procesamiento y Análisis de Datos ............................................................................ 52 Sistema de Hipótesis ..................................................................................................................... 52 Hipótesis Nula ............................................................................................................................... 52 Hipótesis Alternativa .................................................................................................................... 53 Análisis y Discusión de Resultados .............................................................................................. 53 Análisis de Resultados .................................................................................................................. 53 Análisis del Suelo e Identificación de Bacterias a Partir de Muestras de Rizosfera de Plantas de P. Americana por Medio de Técnicas Microbiológicas ................. 53 Determinación de la Producción de Sideróforos en Rizobacterias a Través de Ensayos Cualitativos ................................................................................................................................... 56 Identificación de los Genes Relacionados con la Síntesis de Sideróforo en Rizobacterias Mediante el Diseño de Cebadores y la Estandarización de la PCR .............. 56 Identificación Bioquímica y Molecular de Bacterias Productoras de Sideróforos ....................... 60 Discusión....................................................................................................................................... 62 Conclusiones ................................................................................................................................. 65 Referencias Bibliográficas ............................................................................................................ 66 Apéndices ...................................................................................................................................... 82spa
dc.format.extent86 p.
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/msword
dc.identifier.instnameUniversidad de Santander
dc.identifier.localT 17.24 G476p
dc.identifier.reponameRepositorio Digital Universidad de Santander
dc.identifier.repourlhttps://repositorio.udes.edu.co
dc.identifier.urihttps://repositorio.udes.edu.co/handle/001/11697
dc.language.isospa
dc.publisherUniversidad de Santander
dc.publisher.branchCúcuta
dc.publisher.facultyFacultad de Ciencias Médicas y de la Salud
dc.publisher.placeCúcuta, Colombia
dc.publisher.programBacteriología y Laboratorio Clínico
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dc.rightsDerechos Reservados - Universidad de Santander, 2024. Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.proposalBiofertilizantesspa
dc.subject.proposalBiocontrolspa
dc.subject.proposalHierrospa
dc.subject.proposalRizobacteriasspa
dc.subject.proposalSideróforosspa
dc.subject.proposalPersea americanaspa
dc.subject.proposalBiofertilizerseng
dc.subject.proposalBiocontroleng
dc.subject.proposalRhizobacteriaeng
dc.subject.proposalSiderophoreseng
dc.subject.proposalPersea americanaeng
dc.titleProducción de Sideróforos en Bacterias Aisladas de la Rizosfera del Cultivo de Persea americana en Chinácota, Norte de Santanderspa
dc.title.translatedSiderophore Production in Bacteria Isolated from the Rhizosphere of Persea Americana Cultivation in Chinácota, Norte de Santander
dc.typeTrabajo de grado - Pregrado
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.type.coarversionhttp://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TP
dc.type.versioninfo:eu-repo/semantics/submittedVersion
dcterms.audienceTodas las Audiencias
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