Examinando por Autor "Tofiño Rivera, Adriana Patricia"
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- PublicaciónAcceso abiertoActividad antimicrobiana de aceites esenciales de Lippia alba y cymbopogon citratus sobre streptococcus mutans y citotoxicidad en células cho(2016) Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Mena Álvarez, O.; Martínez Pabón, M.C.; Galvis Pareja, D.; Merini, Luciano J.Background: Dental caries is a complex in- fectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary f low, buffering capa- city and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not com- pletely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capabi- lity of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromato- graphy with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mu- tans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium. Results: The major components in both essential oils were Geraniol and Citral. Lippia alba essential oil applied in concen- tration of 0.01 mg / 100 mL removed 95.8% of S. mutans biofilm and C. citratus e s s ent i a l o i l s h o w e d a removal activity of 95.4% in the concentrations 0.1, 0.01mg /100 mL and 93.1% in concentration 0.001 mg / 100 mL. None of the essential oils showed toxicity to CHO cells in a 24-hour treatment, with signif icant differences in relation to the control with methanol (P = 0.00) which inhibits most cells. Conclusions: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biof ilms and null cytotoxicity, evidencing a po- tential use in treating and preventing dental caries.
- PublicaciónAcceso abiertoDeterminación del potencial productivo y nutricional de un frijol biofortificado mesoamericano en el cesar, Colombia(2012-01) Tofiño Rivera, Adriana Patricia; Tofiño, Rodrigo; Jiménez Rios, HedilkaSe caracterizó agronómica y nutricionalmente frijoles biofortificados; se evaluó el SMN18 sensorialmente y el efecto de la ingesta en niños de 7-11 años en Valledupar. El objetivo fue identificar el impacto de la inclusión del frÌjol biofortificado en el menú de un comedor escolar, encontrandose que los biofortificados presentaron rendimiento, sanidad, contenido nutricional y diferencias sensoriales superiores con respecto al testigo. Además, su ingesta mejoró el nivel de hemoglobina, transferrina e Ìndice de masa corporal.
- PublicaciónAcceso abiertoEfectividad de Beauveria bassiana (Baubassil®) sobre la garrapata común del ganado bovino Rhipicephalus microplus en el Departamento de la Guajira, Colombia(2018-05-07) Tofiño Rivera, Adriana Patricia; Pedraza Claros, Bertilda; Ortega Cuadros, Mailen; Perdomo Ayola, Sandra C.; Moya Romero, Diana CarolinaThe effectiveness of Baubassil® (Beauveria bassiana) on the tick Rhipicephalus (Boophilus) microplus was evaluated. In the in vitro phase, the optimal germination temperature of the fungus was determined by microcultures at 25, 30 and 35 °C and the mortality of ticks. In the field, in a randomized design, 30 cebuine cows; 15 cows were treated with Baubassil® once a week at a 1 × 106 conidia/ml, and a control group was left untreated. It was observed that at temperatures of 25, 30 and 35 °C, germination of the entomopathogen was 97.5, 94.7 and 6.3%, respectively. In animals treated with Baubassil® obtained a corrected effectiveness of 96.8% on ticks in the fifth week of application. The use of Baubassil® would be a valid alternative for controlling the cattle tick under the agroecological conditions of the Dry Caribbean.
- PublicaciónAcceso abiertoEffect of agricultural inputs and essential oils on the soil of vegetables in Colombia's Caribbean region(2018) Tofiño Rivera, Adriana Patricia; Melo Ríos, Aslenis Emidia; Merini, Luciano J.; Ortega Cuadros, Mailen; Mena Rodríguez, EduardoA contribuição das hortaliças para a segurança alimentícia e para o desenvolvimento econômico da Colômbia, bem como para a problemática ambiental mundial, justifica o interesse de elaborar estratégias produtivas sustentáveis para a agrocadeia. Desenvolveu-se um estudo com o objetivo de avaliar o efeito da aplicação de óleos essenciais e agroinsumos em cultivos de ají (Capsicum annuum), feijão e berinjela em Codazzi, Cesar. A metodologia incluiu a análise de compatibilidade de pesticidas utilizados nessas hortaliças e óleos de Lippia alba e Cymbopogon citratus, no que se refere ao efeito biocida in vitro em cepas nativas de Macrophomina phaseolina, Phytophthora capsici e Colletotrichum gloeosporioides. Em campo, aplicou-se tiabendazol de maneira individual e combinado com os óleos. Foram medidos indicadores físico-químicos e microbiológicos do solo, a incidência de pragas e doenças, e o desempenho dos cultivos. In vitro, os óleos controlaram até 97 % dos fitopatógenos e mostraram compatibilidade com carbendazim e tiabendazol. Em campo, foi observado um controle de doenças de até 67 % com C. citratus + tiabendazol a respeito do grupo controle (p = 0,00), desempenhos próximos à média regional, e melhores condições microbiológicas e físico-químicas do solo. Em conclusão, existem diferenças no efeito edáfico entre tratamentos, já que o agroquímico e a combinação de óleos foram mais favoráveis que o efeito individual de cada produto nas variáveis avaliadas. Isso leva a continuar realizando. avaliações com óleos em campo, para dilucidar a duração dos efeitos descritos.
- PublicaciónAcceso abiertoEffect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells(2016-10-17) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Galvis Pareja, D.; Jiménez Rios, Hedilka; Merini, Luciano J.; Martínez Pabón, M.C.Background Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Chemical compounds studied in this article List of up to 10 names of chemical compounds studied in the article. Geranial. Neral. Myrcene. Geraniol. (E)-Caryophyllene. trans-Verbenol. Geranyl acetate. cis-Verbenol. Germacrene D. 37-Dimethyl-26-octadiene-1-ol
- PublicaciónAcceso abiertoEvaluacion agronómica y sensorial del frijol(Phaseotus vulgaris l) mejorados nutricionalmente en el norte del departamento del Cesar, Colombia(2011-07) Tofiño Rivera, Adriana Patricia; Tofiño, Rodrigo; Cabal, Diana Carolina; Melo Ríos, Aslenis Emidia; Camarillo, William; Pachón, HelenaObjetivo: evaluar las características agronómicas y sensoriales de varios genotipos de fríjol (Phaseolus vulgaris L.) biofortificado, con respecto a un tradicional, para seleccionar el de mejores posibilidades de adopción. Materiales y métodos: el estudio se desarrolló en el Cesar (Colombia) y comprendió tres fases: 1) evaluación de la aceptación de semillas de 35 líneas de fríjol biofortificado por 331 productores y consumidores, 2) evaluación agronómica y nutricional, de las nueve líneas mejor calificadas y 3) evaluación sensorial por 273 personas comparativamente con un testigo regional. A los cuatro genotipos con mejores resultados agronómicos y nutricionales se les aplicaron pruebas de aceptabilidad, al mejor valorado, además pruebas de preferencia y discriminación triangular. Resultados: los de mejor aceptación por tipo de semilla fueron dos genotipos de cada color: negro, crema, rojo y rosado moteado. Los cuatro con mejor rendimiento, sanidad, contenidos de hierro y zinc incluyeron un genotipo de cada color; frente a un patrón local no presentaron diferencias de aceptabilidad por olor, color y sabor (p>0,05), pero si por textura y consistencia (p<0,05) a favor de los biofortificados, sobresaliendo el negro. Con este último y el patrón local se preparó una receta, los evaluadores discriminaron entre los dos y hubo diferencias en la aceptabilidad de algunas características sensoriales, a favor del biofortificado. Conclusión: los genotipos de fríjol biofortificado presentaron mejor rendimiento, sanidad, contenidos de hierro y zinc que el patrón local, los cuatro mejor valorados presentaron algunas diferencias sensoriales frente al testigo, a favor de los biofortificados, sobresaliendo el genotipo negro.
- PublicaciónAcceso abiertoEvaluation of agrochemicals and bioinputs for sustainable bean management on the Caribbean coast of Colombia(2015) Melo Ríos, Aslenis Emidia; Ariza, Pedro; Lissbrant, Sofía; Tofiño Rivera, Adriana PatriciaThe sustainable expansion of bean cultivations requires technologies that do not limit their phyto-recovering properties. Therefore, the objective of this study was to propose agronomic management of conservation for bean cultivation considering the microbiological characteristics of two mega-environments of the Colombian Caribbean coast and the compatibility between agrochemicals and bioinputs. The methodology included rhizospheric microbe population counts, identification of phytopathogenic fungi in plant tissues and soils, compatibility studies of pesticides with biocontrollers, and determination of residual contents of pesticides in bean seeds. The microbial populations corresponded to those previously registered for the lower tropics, but with quantitative differences in the genera. Phytophthora, Colletotrichum and Fusarium were registered in the humid Caribbean, while Colletotrichum and Curvularia affected crops in the dry Caribbean. The Beauveria bioinput was not compatible with the evaluated agrochemicals, while Trichoderma was compatible with chlorpyrifos, thiabendazole and oxycarboxin. Metarhizium was compatible with glyphosate and oxycarboxin at 10% of the recommended dose. Lindane residues were found in the beans harvested at three of the studied locations. The combined use of agrochemicals and bioinputs on bean crops is feasible as long as the time of application of the latter is made according to the half-life of the chemical and the organic matter content of the soil is increased.
- PublicaciónAcceso abiertoHighly nutritional cookies based on a novel beancassava-wheat flour mix formulation(2014) Cabal, Diana Carolina; Melo Ríos, Aslenis Emidia; Lissbrant, Sofía; Gallego, Sonia; La O Hechavarría, María de la Luz; Tofiño Rivera, Adriana PatriciaNutritional deficiencies are common among children in Colombia, and innovative strategies and supplements are needed in order to effectively address this problem. For example, in Colombia, when measured as ferritin, iron deposits are deficient in 58.2% of children between two and eight years of age. If a formulation is made with highly nutritional ingredients, cookies will have the potential to be used as supplements in children's diets because of their simple manufacturing process, long shelf life, and high acceptability. This study aimed to develop biofortified cookies, based on a bean-cassava-wheat flour mix, for children. The methodology grouped several studies in order to define the best treatment for the production of bean flour and the flour mix to produce cookies, prioritizing the nutritional content and the microbiological and sensorial quality. A production procedure for bean-based flour, suitable for the production of cookies with adequate nutritional, sensorial and microbiological characteristics was obtained. Additionally, the rheological characteristics of the proposed flour mixes permitted other possible uses for the bread-making industry, substituting cereal flours with flours with higher micronutrient contents. However, further studies are needed to determine the nutritional effects of the regular ingestion of biofortified cookies on children.
- PublicaciónRestringidoIdentificación de la actividad biológica del aceite esencial l. Alba en los diferentes sectores de la industria, mediante revisión sistemática durante el periodo de 1990 a 2018(Valledupar : Universidad de Santander, 2019, 2019-06-17) Daza Padilla, Angélica Julieth; Tofiño Rivera, Adriana Patricia; Pedraza Claros, BertildaThe Lippia alba, is an aromatic plant, commonly known as prontoalivio in Colombia, of great interest in the national and international market for its various uses especially as an essential oil. In order to identify the perspectives of application of essential oils (AE) of Lippia alba (Mill.) In the productive sectors of the Cesar department, an exploratory review of its registered biological activity was carried out from 1990 to October 2018. The methodology included the search of literature in the EBSCO, Embase, Pubmed, Scopus, Scielo and Lilacs databases. of which was obtained as a result that among the most outstanding researchers are: Bernardo Baldisserotto as the most prolific author with 11 publications, the federal university of Santa Maria stood out as the most relevant institution in number of publications (21), followed by the industrial university of Santander (9), the university of Cartagena (7) and the federal university of Ceará (6), countries such as Brazil (60.5%), Colombia (25.2%) and India (6.2%); They stood out for their research linked to the biomedical sector (46.2%) and industrial (37.3%) mainly; in which they used microorganisms, fish, arthropods, small mammals, and cell lines fundamentally, evaluated by the contact method and with positive results in oils extracted by hydrodistillation (55.3%) and steam distillation (18, 4%) as the most frequent techniques. The biological activity of the EA was associated with Carvona (30.2%) and linalool (23.6%), in relation to its antibacterial, antifungal, cytotoxic, antioxidant and sedative effects, among others. E. coli was identified as a microorganism highly sensitive to EA of L. alba. The biotechnological articulation of AE of L. alba suggests research focused on the fishing, agrifood and public health industries.
- PublicaciónAcceso abiertoVigilancia tecnológica de plantas aromáticas : De la investigación a la consolidación de la agrocadena colombiana(2017-05) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Melo Ríos, Aslenis Emidia; Mier Giraldo, Helen JhoanaEl sector de plantas aromáticas, medicinales y condimentarias reúne una amplia variedad de especies nativas e introducidas con potencial agroindustrial. Para contribuir al diagnóstico tecnológico del sector en Colombia y brindar elementos de planificación estratégica hacia mejores niveles de desarrollo e internacionalización, se priorizaron ciertas especies aromáticas en este artículo y se planteó una vigilancia tecnología del 2004 al 2014. Se hizo una búsqueda de artículos científicos y patentes en ocho bases de datos en el ámbito nacional, iberoamericano y mundial según temáticas priorizadas. Los datos se procesaron mediante Microsoft Excel, EndNote, Vantage Point (2014) y Thomson Data Analyzer. A nivel nacional, la temática más registrada fue el material de siembra y el mejoramiento genético, mientras que en Iberoamérica y el mundo, fue el efecto biocida. Los países líderes en investigaciones sobre las aromáticas vigiladas fueron Brasil, Colombia y Cuba. A nivel internacional, los líderes en producción científica en PubMed y Scopus fueron India, EE. UU., Brasil y Colombia. La tendencia mundial de patentes es creciente, y su principal enfoque es “Necesidades corrientes de la vida”. En conclusión, la vigilancia tecnológica ofreció una visión global de avances y tendencias en investigación y desarrollo tecnológico en áreas temáticas vinculadas con las plantas aromáticas. Además, se identificaron posibles aliados investigativos y fluctuación de la producción científica a través del tiempo.