BBBA Trabajos de Grado

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Examinando BBBA Trabajos de Grado por Materia "Adaptive Changes"

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    Evaluación de la Patogenicidad de Cryptococcus Neoformans Serotipo Vni en Galleria Mellonella In Vitro en el 2018.
    (Cúcuta: Universidad de Sántander, 2019, 2019-07-04) Avellaneda Figueroa, Luis Alfredo; Contreras Niño, Elizabeth; Angarita Sanchez, Asbleide Karina.; Contreras Rangel, Jael.
    Cryptococcosis is an opportunistic mycosis of great interest, especially because of its associated presentation in patients with HIV. Studies carried out by the Group of works of the UDES, have allowed the conformation of a bank of strains constituted by clinical and environmental samples of Cryptococcus, it is important to know that there are references on the use of the Galleria mellonella model for the study of the pathogenesis of Cryptococcus neoformans highlighting the advantages of this simple model, unlike the mammals models where their manipulation is more complicated and they are expensive. Therefore, the general objective of the project was the evaluation of the pathogenicity of Cryptococcus neoformans serotype VNI in Galleria mellonella. To this end, two control strains of Cryptococcus (H99 and JEC21) were used, which correspond to the variety grubbi and the variety neoformans, respectively, which, like the strains studied, were incubated and subsequently inoculated in the invertebrate model G. mellonella, Pathogenicity modulation was evaluated by survival curves by the Kaplan mejer statistical method, where the days of death of the larvae caused by each of the clinical and environmental isolates were observed. Additionally, the capsular size of the Cryptococcus studied was evaluated, because the capsule is the main factor of virulence. The capsule was measured in fresh preparations 15 with Chinese ink before and after infection, in the same way the adaptive changes of Cryptococcus neoformans were evaluated, submitting them to an oxidative stress with NaOH, HCl and Menadione. This work made important contributions on the pathogenicity characteristics of both clinical and environmental Cryptococcus strains of the region, thus contributing to the development of studies of Cryptococcosis.