Examinando por Materia "Essential oils"

Mostrando 1 - 9 de 9
  • Publicación
    Acceso abierto
    Actividad antimicrobiana de aceites esenciales de Lippia alba y cymbopogon citratus sobre streptococcus mutans y citotoxicidad en células cho
    (2016) Ortega Cuadros, Mailen; Tofiño Rivera, Adriana Patricia; Mena Álvarez, O.; Martínez Pabón, M.C.; Galvis Pareja, D.; Merini, Luciano J.
    Background: Dental caries is a complex in- fectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary f low, buffering capa- city and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not com- pletely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capabi- lity of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromato- graphy with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mu- tans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium. Results: The major components in both essential oils were Geraniol and Citral. Lippia alba essential oil applied in concen- tration of 0.01 mg / 100 mL removed 95.8% of S. mutans biofilm and C. citratus e s s ent i a l o i l s h o w e d a removal activity of 95.4% in the concentrations 0.1, 0.01mg /100 mL and 93.1% in concentration 0.001 mg / 100 mL. None of the essential oils showed toxicity to CHO cells in a 24-hour treatment, with signif icant differences in relation to the control with methanol (P = 0.00) which inhibits most cells. Conclusions: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biof ilms and null cytotoxicity, evidencing a po- tential use in treating and preventing dental caries.
  • Publicación
    Acceso abierto
    Caracterización del efecto de mezclas de terpenos derivados de aceites esenciales de Lippia alba y agentes quimioterapéuticos sobre células de cáncer hematológico
    (Bucaramanga : Universidad de Santander, 2020, 2020-03-13) Vargas Munévar, Laura Camila; Moreno Moreno, Erika Marcela
    El cáncer constituye la segunda causa de muerte a nivel mundial, siendo las leucemias el tipo de cáncer hematológico más representativo en niños y adultos, con una cifra aproximada de 309.006 defunciones y 437.033 nuevos diagnósticos. Actualmente, el tratamiento de estas neoplasias se caracteriza por ser altamente tóxico, con desenlace en múltiples y graves efectos colaterales. Por tanto, el desarrollo de terapias farmacológicas basadas en moléculas bioactivas de plantas es prometedor teniendo en cuenta sus propiedades antiproliferativas y baja toxicidad. En este contexto, este trabajo caracterizó el efecto de mezclas de terpenos, derivados de aceites esenciales de Lippia alba, limoneno y citral, con los medicamentos oncológicos citarabina y doxorrubicina. Los resultados obtenidos de las interacciones farmacológicas fueron expresados por medio de isobologramas de radio fijo y la detección de cambios morfológicos y de potencial de membrana mitocondrial fue llevado a cabo por medio de microscopía óptica y de fluorescencia, respectivamente. En este trabajo, se encontró que en células de LMA la combinación con mejor perfil fue limoneno+citarabina (ΣCIF=0,27), demostrando además efecto antagónico (protector) sobre el linaje no tumoral (ΣCIF=1,15). Por otra parte, en células de LMC, la mejor interacción fue limoneno+citral (ΣCIF=0,46); sin embargo, es importante destacar que la mezcla citral+doxorrubicina, representó la segunda combinación de mayor eficacia (ΣCIF=0,54) y, además, evidenció un efecto antagonista sobre las células Vero (ΣCIF =1,18). En todas las combinaciones testadas se evidenciaron cambios morfológicos posiblemente de tipo apoptótico. Estos resultados permiten sugerir que las moléculas bioactivas podrían ser utilizadas como coadyuvantes de la terapia convencional, potencializando su efecto antiproliferativo de manera selectiva contra células de la LMA y LMC.
  • Publicación
    Acceso abierto
    Differential anti-proliferative effect on K562 leukemia cells of Lippia alba (Verbenaceae) essential oils produced under diverse growing, collection and extraction conditions
    (2017-02) García Sánchez, Liliana Torcoroma; Leal, Andrés Felipe; Moreno Moreno, Erika Marcela; Stashenko, Elena E.; Arteaga, H. José
    Lippia alba (Verbenaceae Family) is an aromatic shrub native to Latin America, with recognized medicinal properties and a well-known high phenotypical plasticity in response to environmental factors. In order to identify the parameters that potentiate its in vitro anti-proliferative effect on human leukemia cells (K562), a matrix was designed for the production of 76 essential oils (EOs) with variations in plant growing season, harvested part from the plant, and preservation and distillation material conditions. The results show that EOs obtained from the same L. alba chemotype (Citral or Carvone), but acquired under different environmental or extraction conditions, presented significant differences in their primary components, oil yield, and cytotoxic activity on K562 cells. Citral EOs produced under diverse conditions displayed a cytotoxic effect on tumour cells ranging from 54 to 95% (IS 1.8–8.6) and IC50 from 13 to 38.8 μg/mL. The differences in antiproliferative activity were significantly correlated with variations in the β-caryophyllene oxide concentration. These results show the value of using standardized production conditions for the commercial utilization of L. alba EOs.
  • Publicación
    Acceso abierto
    Effect of agricultural inputs and essential oils on the soil of vegetables in Colombia's Caribbean region
    (2018) Tofiño Rivera, Adriana Patricia; Melo Ríos, Aslenis Emidia; Merini, Luciano J.; Ortega Cuadros, Mailen; Mena Rodríguez, Eduardo
    A contribuição das hortaliças para a segurança alimentícia e para o desenvolvimento econômico da Colômbia, bem como para a problemática ambiental mundial, justifica o interesse de elaborar estratégias produtivas sustentáveis para a agrocadeia. Desenvolveu-se um estudo com o objetivo de avaliar o efeito da aplicação de óleos essenciais e agroinsumos em cultivos de ají (Capsicum annuum), feijão e berinjela em Codazzi, Cesar. A metodologia incluiu a análise de compatibilidade de pesticidas utilizados nessas hortaliças e óleos de Lippia alba e Cymbopogon citratus, no que se refere ao efeito biocida in vitro em cepas nativas de Macrophomina phaseolina, Phytophthora capsici e Colletotrichum gloeosporioides. Em campo, aplicou-se tiabendazol de maneira individual e combinado com os óleos. Foram medidos indicadores físico-químicos e microbiológicos do solo, a incidência de pragas e doenças, e o desempenho dos cultivos. In vitro, os óleos controlaram até 97 % dos fitopatógenos e mostraram compatibilidade com carbendazim e tiabendazol. Em campo, foi observado um controle de doenças de até 67 % com C. citratus + tiabendazol a respeito do grupo controle (p = 0,00), desempenhos próximos à média regional, e melhores condições microbiológicas e físico-químicas do solo. Em conclusão, existem diferenças no efeito edáfico entre tratamentos, já que o agroquímico e a combinação de óleos foram mais favoráveis que o efeito individual de cada produto nas variáveis avaliadas. Isso leva a continuar realizando. avaliações com óleos em campo, para dilucidar a duração dos efeitos descritos.
  • Publicación
    Acceso abierto
    Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
    (2016-10-17) Tofiño Rivera, Adriana Patricia; Ortega Cuadros, Mailen; Galvis Pareja, D.; Jiménez Rios, Hedilka; Merini, Luciano J.; Martínez Pabón, M.C.
    Background Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Chemical compounds studied in this article List of up to 10 names of chemical compounds studied in the article. Geranial. Neral. Myrcene. Geraniol. (E)-Caryophyllene. trans-Verbenol. Geranyl acetate. cis-Verbenol. Germacrene D. 37-Dimethyl-26-octadiene-1-ol
  • Publicación
    Restringido
    Evaluación de la actividad Antimicrobiana in vitro de un desinfectante a base del aceite esencial de Origanum Vulgare y Zingiber Officinale sobre Escherichia coli en Lactuca Sativa.
    (Cúcuta: Universidad de Santander, 2017, 2017-12-04) Castaño Muñoz, Zully Daniela.; Cote, Jenny.; Diaz Castañeda, Claudia-Elizabeth; Contreras Rangel, Jael.
    El propósito del estudio fue la evaluar la actividad antimicrobiana in vitro de un desinfectante a base del aceite esencial de Origanum vulgare y Zingiber officinale sobre Escherichia coli en Lactuca sativa, teniendo en cuenta la manipulación y de las condiciones de las variables del aceite esencial y de Escherichia coli para evaluar el efecto antimicrobiano del desinfectante. Los resultados obtenidos de los procedimientos aplicados determinaron que la CMI delJengibre Zingiber officinale presenta efectividad a una concentración de 90% y del Origanum vulgare a una concentración de 75%. La CMB del Jengibre Zingiber officinale se evidencia a una concentración de 100%. y del Origanum vulgare a una concentración de 75%. El tiempo de inhibición del Jengibre presento inhibición a las 3 horas con una concentración de 80% del oregano a la hora a 100%. Concluyendo que la eficiencia antibacteriana del desinfectante tanto de jengibre como del orégano en su evaluación final fue del 100% efectiva a 35°.
  • Publicación
    Acceso abierto
    Evaluation of antibacterial activity in essential oils and by-products used in the artisan cosmetic industry
    (2018) Arámbula, Claudia-Ivonne; Diaz Castañeda, Claudia-Elizabeth
    The experimental study allowed us to learn about antibacterial activity in essential oils in Eucalyptus, Rosemary, Myrtle, Orange plants and their by-products used in the industry. Phase 1. Extraction of essential oils through steam distillation. Phase 2. Determination of antibacterial activity through diffusion of the agar disc. Phase 3. Determination of the minimum inhibitory concentration through macrodilution in broth. Phase 4. Product effectiveness tests. The results showed antibacterial activity in eucalyptus and rosemary oils against Escherichia coli at concentrations of 100%, 75% and 50%, whereas for S. aureus only orange and eucalyptus oils were effective at concentrations of 75% and 100%, however, eucalyptus oil was the only one with inhibition against Pseudomonas aeruginosa at 100% concentration. It is concluded that the antibacterial activity found in the EO of orange, eucalyptus, myrtle and their by-products such as myrtle gel opens the door for the development of subsequent studies in the search for its application in the cosmetic industry as a viable alternative to the treatment of skin and gastric system related conditions, given the ability to inhibit the growth of S. aureus and E. coli.
  • Publicación
    Acceso abierto
    Immunomodulation and antioxidant activities aspossible trypanocidal and cardioprotectivemechanisms of major terpenes from lippia albaessential oils in an experimental model of chronicchagas disease
    (2021-11-22) Espinel-Mesa, Denerieth Ximena; González Rugeles, Clara Isabel; Mantilla Hernández, Julio César; Stashenko, Elena E.; Villegas-Lanau, Carlos Andrés; Quimbaya Ramírez, John-Jaime; Torcoroma-García, Liliana; Masira
    In the late phase of Trypanosoma cruzi infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect only the elimination of the parasite, but are ineffective for late stage treatment and for preventing heart damage and disease progression. In vivo trypanocidal and cardioprotective activity has been reported for Lippia alba essential oils (EOs), ascribed to their two major terpenes, limonene and caryophyllene oxide. To investigate the role of antioxidant and immunomodulatory mechanisms behind these properties, chronic-T. cruzi-infected rats were treated with oral synergistic mixtures of the aforementioned EOs. For this purpose, the EOs were optimized through limonene-enrichment fractioning and by the addition of exogenous caryophyllene oxide (LIMOX) and used alone or in combined therapy with subtherapeutic doses of BNZ (LIMOXBNZ). Clinical, toxicity, inflammatory, oxidative, and parasitological (qPCR) parameters were assessed in cardiac tissue. These therapies demonstrated meaningful antioxidant and immunomodulatory activity on markers involved in CCC pathogenesis (IFN-γ, TNF-α, IL-4, IL-10, and iNOS), which could explain their significant trypanocidal properties and their noteworthy role in preventing, and even reversing, the progression of cardiac damage in chronic Chagas disease.
  • Publicación
    Acceso abierto
    Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (Citral, limonene and caryophyllene oxide)
    (2018-07-27) Moreno Moreno, Erika Marcela; Leal Pinto, Sandra Milena; Stashenko, Elena E.; García Sánchez, Liliana Torcoroma
    Background Chagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease’s progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal plants as Lippia alba, offer a promising reserve of bioactive molecules. The principal goal of this work is to characterize the inhibitory properties and cellular effects of the Citral and Carvone L. alba chemotype essential oils (EOs) and their main bioactive terpenes (and the synergies among them) on T. cruzi forms. Methods Twelve L. alba EOs, produced under diverse environmental conditions, were extracted by microwave assisted hydrodistillation, and chemically characterized using gas chromatography coupled mass spectrometry. Trypanocidal activity and cytotoxicity were determined for each oil, and their major compounds, on epimastigotes (Epi), trypomastigotes (Tryp), amastigotes (Amas), and Vero cells. Pharmacologic interactions were defined by a matrix of combinations among the most trypanocidal terpenes (limonene, carvone; citral and caryophyllene oxide). The treated cell phenotype was assessed by fluorescent and optic microscopy, flow cytometry, and DNA electrophoresis assays. Results The L. alba EOs displayed significant differences in their chemical composition and trypanocidal performance (p = 0.0001). Citral chemotype oils were more trypanocidal than Carvone EOs, with Inhibitory Concentration 50 (IC50) of 14 ± 1.5 μg/mL, 22 ± 1.4 μg/mL and 74 ± 4.4 μg/mL, on Epi, Tryp and Amas, respectively. Limonene exhibited synergistic interaction with citral, caryophyllene oxide and Benznidazole (decreasing by 17 times its IC50) and was the most effective and selective treatment. The cellular analysis suggested that these oils or their bioactive terpenes (citral, caryophyllene oxide and limonene) could be inducing T. cruzi cell death by an apoptotic-like mechanism. Conclusions EOs extracted from L. alba Citral chemotype demonstrated significant trypanocidal activity on the three forms of T. cruzi studied, and their composition and trypanocidal performance were influenced by production parameters. Citral, caryophyllene oxide, and limonene showed a possible induction of an apoptotic-like phenotype. The best selective anti-T. cruzi activity was achieved by limonene, the effects of which were also synergic with citral, caryophyllene oxide and benznidazole.